<div><strong>Background:</strong> <em>Mycobacteroides chelonae</em> M77 isolated in a targeted Mycobacterial isolation study from bovine raw milk was whole genome sequenced (CP041150.1), followed by comparative genomics study for better understanding of the organism with special focus on its virulence and antibiotic resistance pattern. This is probably the first whole genome sequence (WGS) of <em>M.cheloane</em> submitted in NCBI database from India.&nbsp;<br />
<br />
<strong>Methods:</strong> The WGS has been carried out in Illumina Next Seq 500 platform and then assembled resulting in a genome of 5.12 MB. The assembled genome was annotated in RASTtk and viewed by SEED for its detailed analysis. Pathogen Finder 1.1 and Res Finder 3.1 soft wares were applied for knowing the isolate pathogenecity and antibiotic profiling respectively. Mauve analysis was carried out to know the genomic re-arrangements.<br />
<br />
<strong>Result: </strong>Pathogen Finder 1.1 showed that M77 was non-pathogenic and Res Finder 3.1 server reveals it is susceptible to most of the antibiotics. Mauve analysis for alignment of isolate revealed no significant re-arrangement of genomic segment. The whole analysis showed that the genome of M77 is tight genome with not much variation in relation to previous complete genomes reported and is non-pathogenic and sensitive to most of the antibiotics.</div>


<div>Whole Genome Sequencing and Analysis of <em>Mycobacteroides chelonae</em> M77 Isolated from Cow Milk from the Hill State of Meghalaya, India</div>


<div><strong>Background:</strong> Collar rot of lentil caused by <em>Sclerotium rolfsii </em>became a major problem for early sown lentil in North-eastern plain zones of India, as it associated with high yield losses every year. The disease cause huge loss in yield due to which, area under this crop is consistently decreasing. In global perspective, losses of 10-20 million dollars associated with <em>S. rolfsii </em>with yield depletion ranging from 1-60% in fields. The resistance and susceptibility of a potential host plant depends on the virulence of isolates of <em>S.rolfsii</em>. So the objectives of this study is to observe the potentiality of different isolates those were collected from major lentil growing districts of West Bengal and tested their virulence on three popular varieties of lentil. However the pathogenecity of <em>S.rolfsii</em> in this region never been tested before. This information could be a strong background for effective management of pathogen for future research.<br />
<br />
<strong>Methods: </strong>Collar rot infected samples were collected from different geographical locations of West Bengal during (Nov-Dec, 2018). And then the collected samples were isolated in Department of Plant Pathology, BCKV, Nadia, W.B. and were confirmed by their morphological characters.&nbsp;<br />
<br />
<strong>Result: </strong>The isolates from different geographical locations showed differences in their virulence. Among the all isolates SRC2 considered as most virulent as it causes highest diseases incidence as well as mortality percentage in all selected varieties of lentil. Two varieties, &nbsp;HUL-57 and BM-6 not so much popular among the farmers but can be consider as resistant to collar rot in case of early sowing condition.</div>


<div>Pathogenicity Study of <em>Sclerotium rolfsii</em> Isolates on Popular Lentil Varieties in Net House Condition</div>


<div>Thirty two isolates of Fusarium oxysporum f.sp. ciceris (Foc) were collected from six districts of Andhra Pradesh (A.P) and three districts of &nbsp;Telangana. Based on wilting % in pathogenicity test, the isolates were grouped into three categories. All the isolates differed in cultural characters like mycelium colour, margin, texture, pigmentation and morphological characters like macro conidia, micro conidia length, width and chlamydospores diameter. There was no correlation between cultural and morphological characters of Foc isolates except three isolates (Foc-6, Foc-12 and Foc-17). These three isolates have white mycelium, cream colour pigmentation, slow radial growth and highly pathogenic (100% wilt incidence) in nature. Sporulation ability and radial growth of all isolates were also measured. &nbsp;All the isolates exhibited genetic variation in RAPD banding pattern and three major clusters (I, II and III) were found in the dendrogram. Maximum genetic similarity of 80.3% was observed between Foc-27 and Foc-28 isolates.</div>


Whole Genome Sequencing and Analysis of Mycobacteroides chelonae M77 Isolated from Cow Milk from the Hill State of Meghalaya, India

Pathogenicity Study of Sclerotium rolfsii Isolates on Popular Lentil Varieties in Net House Condition

Cultural, Morphological and Molecular Characterization of Fusarium Oxysporum f.sp. Ciceris Inciting Wilt of Chickpea