Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 55 issue 6 (june 2021) : 642-646

Whole Genome Sequencing and Analysis of Mycobacteroides chelonae M77 Isolated from Cow Milk from the Hill State of Meghalaya, India

Esther Vise, Sandeep Ghatak, Akshay Garg, Amarjeet Karam, Chendu Bharat Prasad, A. Arun Prince Milton, Ingudam Shakuntala, Kekungu Puro, Raj kumari Sanjukta, Arnab Sen, Samir Das
1Division of Animal Health, ICAR Research Complex for NEH Region, Umiam-793 103, Meghalaya, India. 
Cite article:- Vise Esther, Ghatak Sandeep, Garg Akshay, Karam Amarjeet, Prasad Bharat Chendu, Milton Prince Arun A., Shakuntala Ingudam, Puro Kekungu, Sanjukta kumari Raj, Sen Arnab, Das Samir (2021). Whole Genome Sequencing and Analysis of Mycobacteroides chelonae M77 Isolated from Cow Milk from the Hill State of Meghalaya, India. Indian Journal of Animal Research. 55(6): 642-646. doi: 10.18805/IJAR.B-4007.
Background: Mycobacteroides chelonae M77 isolated in a targeted Mycobacterial isolation study from bovine raw milk was whole genome sequenced (CP041150.1), followed by comparative genomics study for better understanding of the organism with special focus on its virulence and antibiotic resistance pattern. This is probably the first whole genome sequence (WGS) of M.cheloane submitted in NCBI database from India. 
Methods: The WGS has been carried out in Illumina Next Seq 500 platform and then assembled resulting in a genome of 5.12 MB. The assembled genome was annotated in RASTtk and viewed by SEED for its detailed analysis. Pathogen Finder 1.1 and Res Finder 3.1 soft wares were applied for knowing the isolate pathogenecity and antibiotic profiling respectively. Mauve analysis was carried out to know the genomic re-arrangements.
Result: Pathogen Finder 1.1 showed that M77 was non-pathogenic and Res Finder 3.1 server reveals it is susceptible to most of the antibiotics. Mauve analysis for alignment of isolate revealed no significant re-arrangement of genomic segment. The whole analysis showed that the genome of M77 is tight genome with not much variation in relation to previous complete genomes reported and is non-pathogenic and sensitive to most of the antibiotics.
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