<div style="text-align: justify;">Bangana dero &nbsp;is an economically important indigenous cyprinid fish of Manipur, India having maximum standard length of 40cm. For the taxonomic placement of this species among the Labeonin fishes, molecular characterization is conducted by using mitochondrial 16SrRNA gene sequences. Partial sequences of mito. 16SrRNA gene of fifteen cyprinid species of the sub-family Labeoninae were downloaded from NCBI Genbank. The analysed nucleotide sequence lengths were 538 bp. In a total of 811 characters, 194 were conserved sites (monomorphic) and 347 were variable sites (polymorphic) and out of 347 variable sites, 73 were parsimony informative sites and 274 &nbsp;singleton sites. The nucleotide frequencies are 31.4% (A), 22.57% (T), 24.00% (C) and 21.96% (G). The transition/transversion bias (R) is 2.51. The overall mean distance is 0.083. The dendogram constructed by Neighbour-Joining tree have three clusters which can indicate the taxonomic positions of Bangana dero .</div>

<div style="text-align: justify;">Molecular characterization of an indigenous minor carp Bangana dero (Cyprinidae: Labeoninae) of Manipur and its relationship with Labeonin fishes of North East India based on mitochondrial 16SrRNA gene sequences</div>

Background: Bangana dero is a cyprinid fish species that is classified under the subfamily Labeoninae. It occurs in the streams of China, India and Nepal near the foot of the Himalayan range. B. dero&rsquo;s whole mitochondrial genome has been sequenced and annotated. In this work, the tRNAs, protein-coding genes have been characterized and analyzed the phylogenetic relation of this fish with other cyprinid species. 
 
Methods: Nanopore sequencing was utilized to determine the entire mitochondrial genome of B. dero. Utilizing NEBNext Ultra DNA Library Prep Kit, end-repair was performed on the isolated DNA using the. Barcoding adapter ligation (BCA) and cleaned. Sequencing was done. All the long read bases were trimmed based on lowest base quality score, Q&lt;9. Barcode and adapters sequences were also trimmed using a porechop programme and the statistical graph illustrations were generated and genome assembly was done using Canu1.5. 
 
Result: The mitogenome is 16613 bp and contains 13 protein-coding genes, 2 rRNA genes,&nbsp; 22 tRNA genes, as well as a non-coding region. The nucleotide makeup is A=32.5%, T=25.21%, C=26.51% and G=15.77%, G + C = 42.28% and A+T=57.71%. The clover-leaf secondary structure was found in all tRNAs except tRNA-Ser2, which has no dihydrouridine arm. For 12 out of 13 protein-coding genes, the initiation codon was ATG, but COI used GTG. Methionine and tryptophan are unique among amino acids as they are each encoded by a single codon. Origin of light chain replication (OL) could fold into a hairpin secondary structure and carried a sequence 5&#39;-GGCGG-3&#39; in its stem. A long intergenic spacer of 46 bp was observed between tRNA-Leu1 and ND1. In the phylogenetic study, B. dero clustered closely with the Bangana species (AP013327) and Labeo boggut (NC_029450), a grouping supported by a bootstrap value of 100. This is the first study on the entire mitochondrial DNA analysis of B. dero.

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Molecular characterization of an indigenous minor carp Bangana dero (Cyprinidae: Labeoninae) of Manipur and its relationship with Labeonin fishes of North East India based on mitochondrial 16SrRNA gene sequences

The Complete Mitochondrial Genome of Bangana dero (Cyprinidae: labeoninae) and its Relationship with Other Labeonin Fishes