Molecular characterization of an indigenous minor carp Bangana dero (Cyprinidae: Labeoninae) of Manipur and its relationship with Labeonin fishes of North East India based on mitochondrial 16SrRNA gene sequences

DOI: 10.18805/ijar.B-3554    | Article Id: B-3554 | Page : 594-598
Citation :- Molecular characterization of an indigenous minor carp Bangana dero (Cyprinidae: Labeoninae) of Manipur and its relationship with Labeonin fishes of North East India based on mitochondrial 16SrRNA gene sequences.Indian Journal Of Animal Research.2019.(53):594-598
Ch. Basudha, N. Sobita, A. Rawat and N. Prakash n_sobita@yahoo.co.in
Address : ICAR Research Complex for NEH Region Manipur Centre Lamphelpat, Imphal-795 004, Manipur, India.
Submitted Date : 30-12-2017
Accepted Date : 1-06-2018

Abstract

Bangana dero  is an economically important indigenous cyprinid fish of Manipur, India having maximum standard length of 40cm. For the taxonomic placement of this species among the Labeonin fishes, molecular characterization is conducted by using mitochondrial 16SrRNA gene sequences. Partial sequences of mito. 16SrRNA gene of fifteen cyprinid species of the sub-family Labeoninae were downloaded from NCBI Genbank. The analysed nucleotide sequence lengths were 538 bp. In a total of 811 characters, 194 were conserved sites (monomorphic) and 347 were variable sites (polymorphic) and out of 347 variable sites, 73 were parsimony informative sites and 274  singleton sites. The nucleotide frequencies are 31.4% (A), 22.57% (T), 24.00% (C) and 21.96% (G). The transition/transversion bias (R) is 2.51. The overall mean distance is 0.083. The dendogram constructed by Neighbour-Joining tree have three clusters which can indicate the taxonomic positions of Bangana dero .

Keywords

Labeoninae Mitochondrial 16SrRNA gene Molecular phylogeny Taxonomic relationship

References

  1. Arunkumar, L. (2008). Bangana chindwinicus, a new species of cyprinid fish (Cypriniformes: Cyprinidae) from Manipur, India. Aquacult 9(1): 13-22.
  2. Brown, W. M., Matthew George, J.R. and Wilson, A.C. (1979). Rapid evolution of animal mitochondrial DNA. Proceedings of National Academy of Science. 76: 1969-1971.
  3. Cavender, T.M., Coburn M.M.(1992). Phylogenetic relationships of North American Cyprinidae. In: Systematics Historical Ecology and North American Freshwater Fishes Mayden RL (ed). Stanford University Press, Palo Alto, CA, pp 293–327
  4. Coad, B.W. (1995). Freshwater fishes of Iran. Acta Sci. Nat. Acad. Sci. Brno. 29(1): 1-64.
  5. Chen, X., Yue, P. and Lin, R. (1984). Major group within the family Cyprinidae and their phylogenetic relationships. Acta Zootaxon Sin. 9: 424-440.
  6. Gilles, A., Lecoitre, G., Faure, E., Chappaz, R. and Brun, G. (1998). Mitochondrial phylogeny of the European cyprinids:implications for their systematic reticulate evolution and colonization time. Molecular Phylogenetics Evolution. 10: 132–143.
  7. Gilles, A., Lecointre, G., Miquelis, A., Loerstche,r M., Chappaz R. and Brun, G. (2001). Partial combination applied to phylogeny of European cyprinids using the mitochondrial control region. Mol. Phylogenet. Evol. 19 : 22-33.
  8. Gosline, W. A. (1978). Unbranched dorsal-fin rays and subfamily classification of the fish family Cyprinidae. Occas. Pap. Mus. Zool Univ. Mich. 684: 1-21.
  9. Howes, G. (1991). Systermatics and biogeography: an overview. In: Cyprinids Fishes: Systematic Biology and Exploitation. Win I., Nelson J., (Eds). Chapman and Hall, New York:pp. 1-54.
  10. Hudson, R., Boos, D. D. and Kaplan, N. L. (1992). A statistical test for detecting population subdivision. Mol Biol Evol. 9:138-151. 
  11. Li, Y., Ren, Z., Shedlockm, A.M., Wu, J. Sang, L. and Tersing, T. (2013). High altitude adaptation of the Schizothoracine fishes (cyprinidae) revealed by the mitochondrial genome analysis. Gene 517:169-178.
  12. Moritz, C., Dowling, T.E. and Brown, W. M. (1987). Evolution of animal mitochondrial DNA: relevance for population biology andsystematic. Annu Rev Ecol Syst. 18: 269-292.
  13. Nelson, J. S.(2006). Fishes of the World. 4th ed. Hoboken: John Wiley & Sons. (New Jersey, USA) xix+601 p.
  14. Palumbi, S. R. (1996). Nucleic acids II: The polymerase chain reaction. In: Molecular systematics. [Hillis DM, Moritz C, Mable BK(eds)]. Sinauer Associates, Inc, : 205–247
  15. Petr, T. (1999). Coldwater fish and fisheries in Afganistan. p.138-148 In: Fish and Fisheries at Higher Aititudes: Asia T. Petr (ed.). FAO Fish. Tech. Pap.No. 385. FAO, Rome, 304p.
  16. Rahman, A. K. A.(1989). Freshwater fishes of Bangladesh. Zoological Society of Bangladesh. Department of Zoology, University of Dhaka. 364p.
  17. Rosenberg, M. S., Subramanian, S. and Kumar, S. (2003). Patterns of transitional mutation biases within and among mammalian genomes. Mol Bio Evo . 20(6):988-993. 
  18. Shreshtha, J. (1999). Coldwater Fish and Fisheries in Nepal. FAO Fish. Tech. Pap. 385: 13-40.
  19. Sobita, N. and Basudha, Ch. (2017). Molecular phylogeny of Barbin fishes of North-East India based on mitochondrial 16SrRNA gene sequences. Indian Journal of Animal Research. 51(6):1062-1065.
  20. Talwar, P.K. and Jhingran A.G., (1991). Inland Fishes of India and Adjacent Countries. Vol 1. A.A. Balkema, Rotterdam. 541 p.
  21. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood evolutionary distance and maximum parsimony methods. Mol Bio Evo. 28(10):2731-2739.
  22. Topal, M. D. and Fresco, J. R. (1976). Complementary base pairing and the origin of substitution mutations. Nature 263:285-293. 
  23. Vishwanth, W. (2002). Fishes of North East India. A Field Guide to Species Identification. Department of Life Sciences, Manipur University, Imphal, India. 129 p.
  24. Vishwanath, W., Nebeshwar, K., Lokeshwaor, Y., Shangningam, B.D.and Rameshori, Y. (2014). Freshwater fish taxonomy and a manual for identification of fishes of Northeast India. National Workshop on freshwater fish taxonomy, Department of Life sciences, Manipur University, September 22-26: 14-28.
  25. Zhu, S.Q.(1995). Synopsis of Freshwater Fishes of China. Jiangsu Science and Technology Publishing House i-v +1-549. 

Global Footprints