Background: The human skin is a natural barrier that protects against external stress, but its physiological structure can be compromised when it gets damaged. In the field of medical science, wounds are a significant issue that requires immediate attention. Lavandula species are used in the food, perfume and cosmetics industries due to their antimicrobial, anti-inflammatory and wound-healing properties.&nbsp; 
 
Methods: The present study was done to assess the biocompatibility, antimicrobial and wound-healing potential of L. dentata leave methanolic extract (LE) that is cultivated in the Taif region of Saudi Arabia. The biocompatibility of LE was analyzed against the normal human skin fibroblast (HSF) cell line using SRB assay. A quarter of LE IC50 was used for wound healing assay. Finally, Escherichia Coli, Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus pyogenes were used to determine LE antibacterial potential through the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) measurement.&nbsp; 
 
Result: LE appears safe on HSF cell line with IC50 = 97 mg/mL. LE decreased wound width, wound area and the mean cell migratory rate of wound border cells during scratch closure, while increased the wound closure% in a time-dependent manner compared to negative control. LE shows antimicrobial potential against all four strains at MIC and MBC&gt;3.0 mg/mL, except for Streptococcus pyogenes MIC= 3.0 mg/mL. In conclusion, LE offers therapeutic benefits against wound healing with an antimicrobial effect, however, higher concentrations of LE should be used to assess its effect.

Biocompatibility, Antimicrobial and Wound Healing Activities of Lavandula dentata Leaves Extract

<div>Background: Under current scenario of High cost of Production of Sugarcane crop, it is need of time to decide specific seed rate for individual varieties. Sugarcane seed contributes to 10-20% of cost of production and farmers invests about Rs. 20000/- per acre on planting material. The costs of seed can be curtailed by optimizing seed rate. Similarly, seed rate varies with different varieties. In this view, a field experiment was carried out to optimize the seed rate of promising sugarcane clones. 
 
Methods: A field experiment was carried using randomized complete block design with factorial arrangements having three replications at Sugarcane Research Institute, Faisalabad during 2016-17 and 2017-18.&nbsp; 
 
Result: The results revealed that maximum cane diameter was recorded for S2008-AUS-133 at seed rate of 25000 and 50000 triple budded setts/ha. However; maximum number of canes/ha were recorded for S2008-AUS-133 at seed rate of 75000 setts/ha followed by HSF-240 at 75000 setts/ha. Maximum cane yield was noted for HSF-240 at seed rate of 75000 followed by S2008-AUS-133 at seed rate of 50000 and lowest can yield was recorded in S2003-US-127 at seed rate of 25000 triple budded setts/ha. Genotypic and phenotypic variances were higher for plant height (381.63, 133.91 cm) and cane yield (406.50, 330.65 kg/plot).Genotypic and phenotypic coefficient of variances were moderate for number of tillers (11.6%, 15.44%) and higher for cane yield (20.24%, 22.44%). It means that variability in 4 genotypes for cane yield was higher. Thus variety S2008-AUS-133 showed better performance at seed rate 75000 while during selection, number of tillers and height should be considered for further breeding program.&nbsp;</div>

<div>Optimizing Seed Rates and Effects of Direct and Indirect Selection Indices in Sugarcane Crop</div>

<div>Background: ROS ( Reactive Oxygen Species) accumulated&nbsp; during heat stress induced oxidative stress is involved in triggering the activation of transcription factor, heat shock factor-1 (HSF-1) which further results in the up regulation of HSP. A cooperation between PGC1&alpha; and HSF1 in the induction of HSPs in response to thermal stress was reported in mice. Thus, the present study was conducted to investigate the effect of HS (Heat Stress) on HSP-70 and PGC-1&alpha; expression in broilers.&nbsp;&nbsp; 
 
Method: The study was conducted in two phases, one during autumn and the other during summer with a total of 300 birds. During the first phase, 60 chicks were divided into six replicates with 10 birds in each and during the second phase, 240 chicks were divided into four groups with six replicates containing 10 birds in each. The experimental rations given to different groups were as control (Basal ration), HS I (Basal ration), HS II (Basal ration+0.3 ppm Se, HS III (Basal ration+0.6 ppm Se) and HS IV (Basal ration+0.9 ppm Se). Hepatic tissues were collected for analysis at 21 d and 42 d. 
 
Result: The MDA levels and expression of HSP-70 protein in hepatic tissues were increased due to HS both at 21 and 42 d, while the expression of PGC-1&alpha; protein increased at 42 d. Se supplementation at 0.3 ppm was effective in decreasing the concentration of MDA and HSP-70 protein at 21d, whereas 0.6 ppm and 0.9 ppm were effective at 42d. Se supplementation decreased the expression of PGC-1&alpha; protein at 42 d during HS.</div>

Biocompatibility, Antimicrobial and Wound Healing Activities of Lavandula dentata Leaves Extract

Optimizing Seed Rates and Effects of Direct and Indirect Selection Indices in Sugarcane Crop

Effect of Organic Selenium during HS in Broiler Chicken: The Interplay of HSP-70 and PGC-1α