<div style="text-align: justify;">An efficient in vitro protocol was developed for callus induction, high frequency plant regeneration through callus cultures derived from cotyledonary leaf and epicotyl explants, rooting of shoots derived from callus and establishment onto the natural conditions in two cultivars of pigeon pea; ICPL 87119 and ICPL 8863. Cotyledonary leaf and epicotyl explants were tested for callus induction across 48 different combinations and concentrations of auxins and cytokinins in MS medium, wherein, higher doses of auxins (15 mg/1 NAA) in combination with lower doses of cytokinins (0.5 mg/l kinetin) induced regenerable callus from leaf explants while lower doses of auxins (0.2 mg/1 NAA) in combination with higher doses of cytokinins (8 mg/1 kinetin) induced regenerable callus from epicotyl explants in both the genotypes. Plantlet regeneration from leaf and epicotyl derived callus was optimized at 0.05 mg/l TDZ in both genotypes. Rooting was optimized on &frac12; MS + 0.5 mg/1 IBA media in both genotypes. Well-rooted plants were acclimatized and established successfully into natural conditions in potting mixture-containing soil: FYM in 1:1 ratio resulting in 48.01 per cent survivability. Regenerated plants were uniform morphologically with normal leaf shape and growth. This protocol finds its significance in rapid multiplication of transgenic plants.</div>

<em>In vitro</em> callus induction and regeneration of multiple shoots through callus derived from leaf and epicotyl explants in pigeon pea (<em>Cajanus cajan</em> L.)

<div style="text-align: justify;">The study was carried out to assess the effect of seed priming to enhance salt tolerance in Algerian Maghreb Cowpea. Seeds of two cowpea landraces (A18 and TZ2) were soaked for 4 h at 25&ordm;C in the dark in distilled water (hydropriming) or 80 mM of NaCl, 80 mM of CaSO4 or 80 mM of CaCl2 (halopriming) separately. Untreated seeds were taken as control (unprimed seeds). Seeds were germinated under three salinity levels (0 (distilled water), 85 mM and 170 mM of NaCl). In general, germination traits of cowpea landraces decreased with increased salinity levels, however, seed priming significantly increased germination percentage, speed of germination, final germination percentage, radical length, hypocotyl length, epicotyl lengh and induced better vigour index under saline and non-saline conditions as compared to the unprimed seed. Hydropriming, or halopriming with NaCl 80 mM or CaSO4 80 mM proved to be effective methods.&nbsp;</div>

<div style="text-align: justify;">Influence of Seed Priming on Early Stages Growth of Cowpea [<em>Vigna unguiculata</em> (L.)Walp.] Grown under Salt Stress Conditions</div>

In vitro callus induction and regeneration of multiple shoots through callus derived from leaf and epicotyl explants in pigeon pea (Cajanus cajan L.)

Influence of Seed Priming on Early Stages Growth of Cowpea [Vigna unguiculata (L.)Walp.] Grown under Salt Stress Conditions