Influence of Seed Priming on Early Stages Growth of Cowpea [Vigna unguiculata (L.)Walp.] Grown under Salt Stress Conditions

DOI: 10.18805/LR-504    | Article Id: LR-504 | Page : 665-671
Citation :- Influence of Seed Priming on Early Stages Growth of Cowpea [Vigna unguiculata (L.)Walp.] Grown under Salt Stress Conditions.Legume Research-An International Journal.2020.(43):665-671
F. Nabi, A. Chaker-Haddadj, M. Chebaani, A. Ghalem, S. Mebdoua, S.M. Ounane fahimanabi@yahoo.fr
Address : Laboratoire productions végétales, Département de production végétale. École Nationale Supérieure Agronomique (ENSA), Avenue Hassan Badi, 16004, El Harrach, Alger Algérie.
Submitted Date : 31-05-2019
Accepted Date : 28-11-2019

Abstract

The study was carried out to assess the effect of seed priming to enhance salt tolerance in Algerian Maghreb Cowpea. Seeds of two cowpea landraces (A18 and TZ2) were soaked for 4 h at 25ºC in the dark in distilled water (hydropriming) or 80 mM of NaCl, 80 mM of CaSO4 or 80 mM of CaCl2 (halopriming) separately. Untreated seeds were taken as control (unprimed seeds). Seeds were germinated under three salinity levels (0 (distilled water), 85 mM and 170 mM of NaCl). In general, germination traits of cowpea landraces decreased with increased salinity levels, however, seed priming significantly increased germination percentage, speed of germination, final germination percentage, radical length, hypocotyl length, epicotyl lengh and induced better vigour index under saline and non-saline conditions as compared to the unprimed seed. Hydropriming, or halopriming with NaCl 80 mM or CaSO4 80 mM proved to be effective methods. 

Keywords

Cowpea Halopriming Hydropriming Salinity tolerance Vigour index

References

  1. Afzal, I., Rauf, S., Basra, S.M.A., Murtaga, G. (2008). Halopriming improves vigor, metabolism of reserves and ionic contents in wheat seedlings under salt stress. Plant Soil Environ. 54 : 382-388. 
  2. Chaker-Haddadj, A., Nabi, F., Ait Kaci-Sadji, H., Ounane, S. M. (2014). Effects of salt stress on growth, nodulation and nitrogen fixation of faba bean (Vicia faba L. Minor) cultivated in Algeria. International Journal of BioTechnology and Research (IJBTR). 4: 1-10.
  3. Gholami, M., Mokhtarian, F., Bahram, B. (2015). Seed halopriming improves the germination performance of black seed (Nigella sativa) under salinity stress conditions. J. Crop Sci. Biotech. 18 (1):21 – 26.
  4. Hall, A. E. (2012). Phenotyping cowpeas for adaptation to drought. Front. Physiol. 3: 155. 
  5. Ibrahim, EA. (2016). Seed priming to alleviatesalinity stress in germinatingseeds. Journal of Plant Physiology. 192: 38-46.
  6. Jafar, M.Z., Farooq, M., Cheema, M.A., Afzal, I., Basra, S.M.A., Wahid, M.A., Aziz, T., Shahid, M. (2012). Improving the performance of wheat by seed priming under saline conditions. J. Agron. Crop Sci. 198: 38-45.
  7. Jorjandi, M., Sharifi-Sirchi, G.R. (2012). The Effect of priming on germination and seedling growth of alfalfa (Medicago sativa L.) under salinity stress. J. Stre. Physiol. Bioch. 8(3): 234-239.
  8. Kaya, M.D., Okcu, G., Atak, M., Cikili Y., Kolsarici, O. (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Europ. J. Agronomy. 24: 291-295.
  9. Kubala, S., Wojtyla, L., Quinet, M., Lechowska, K., Lutts, S., Garnczarska, M. (2015). Enhanced expression of the proline synthesis gene P5CSA in relation to seed osmopriming improvement of Brassica napus germination under salinity stress. Plant Sci. 183: 1–12.
  10. Mahmoudi, H., Ben Massoud, R., Baatour, O., Tarchoune, I., Ben Saleh, I., Nasri, N., Abidi, W., et al. (2012). Influence of different seed priming methods for improving salt stress tolerance in lettuce plants. J. Plant Nutr. 35: 1910-1922.
  11. Nkoana, K.D., Abe Shegro Gerrano Gwata E.T. (2019). Evaluation of diverse cowpea [Vigna unguiculata (L.) Walp.] germplasm accessions for drought tolerance. Legume Research. 42(2) : 168-172.
  12. Memon, N., Gandahi, M.B., Pahoja, V.M., Sharif, N. (2013). Response of seeds priming with boron on germination and seedling sprouts of broccoli. Int. J. Agric. Sci.Res. 3 (2): 183-194.
  13. Miladinov, Z., Maksimovic, I., Baleseviæ-Tubic, S., Djukic, V., Canak, P., Miladinovic, J., Djordjevic, V. (2019). Priming seed mitigates the effects of saline stress in soybean seedlings. Legume Research.
  14. Moghanibashi, M., Karimmojeni, H., Nikneshan, P. (2013). Seed treatment toovercome drought and salt stress during germination of sunflwer (Helianthus annuus L.). J. Agrobiol. 30: 89–96.
  15. Nabi, F., Chaker-Haddadj, A., Tellah, S., Ghalem, A., Ounane, G., Ghalmi, N., Djebbar, R., Ounane, S.M. (2017). Evaluation of Algerian cowpea genotypes for salt tolerance at germination stage. Advances in Environmental Biology. 11(5): 79-88.
  16. Pirasteh Anosheh, H., Sadeghi, H., Emam, Y. (2011). Chemical priming with urea and KNO3 enhances maize hybrids (Zea mays L.) seed viability under abiotic stress. J. Crop Sci. Biotechnol. 14: 289–295.
  17. Rahman, A., Nahar, K., Hasanuzzaman, M., Fujita, M. (2016). Calcium supplementation improves Na+/k+ Ratio, antioxydant defense and glyoxalase systems in salt-stressed rice seedlings. Frontiers in Plant Science. 7: 1-16.
  18. Sadeghipour O. (2017). Amelioration of salinity tolerance in cowpea plants by seed treatment with methyl jasmonate. Legume Research. 40(6): 1100–1106.
  19. Saha, P., Chatterjee, P., Biswas, A.K. (2010). NaCl pretreatment alleviates salt stress by enhancement of antioxidant defense system and osmolyte accumulation in mungbean (Vigna radiata L. Wilczek). Indian J. Exp. Biol. 48: 593-600.
  20. Shahba, M.A., Qian, Y.L., Lair K.D. (2008). Improving seed germination of salt grass under saline conditions. Crop Sci. 48 (2): 756-762.
  21. Sharma, A.D., Rathore, S.V.S., Srinivasan, K., Tyagi, R.K. (2014). Comparison of various seed priming methods for seed germination, seedling vigour and fruit yield in okra (Abelmoschus esculentusL. Moench). Sci. Hortic. 165: 75-81. 

Global Footprints