<div style="text-align:justify">Distribution of CD3, CD79&aacute;cy and S-100, immunohistochemically, in the bursa of Fabricius, thymus and spleen has not been reported in turkeys. Therefore, we determined the localisation of anti-CD3 protein antibodies for mature T-lymphocytes, anti-CD79&aacute;cy antibodies for B-lymphocytes and anti-S-100 protein antibodies for follicular dentritic cells in the bursa of Fabricius, thymus and spleen, which are the lymphoid organs, in a study sample of turkeys. Triple staining method was applied to demonstrate the general structure. Moreover, in all the organs positive reactions were observed with the CD3, CD79&aacute;cy and S-100 antibodies. It was also found that similar areas had a positive reaction with CD79&aacute;cy and S-100 in all of the tested organs. It was remarkable that CD79&aacute;cy reacted positively on Hassall&rsquo;s corpuscles (strongly) and the reticular cells (weakly) in the medulla of the thymus instead of the B-lymphocyte positive areas.&nbsp;</div>

<div style="text-align:justify">Immunohıstochemıcal staınıng of cd3, cd79&alpha;cy and s-100 on bursa fabrıcıus, thymus and spleen of turkeys (<em>Meleagrıs gallapavo</em>)</div>

<div><strong>Background:</strong> The present investigation was carried out on poultry carcasses brought to the Department of Veterinary Pathology, LUVAS, Hisar from Herpesvirus of turkeys (HVT) vaccinated chicken flocks and suspected for viral neoplastic conditions on the basis of necropsy. Conventional and molecular techniques for the confirmation of the Marek&rsquo;s disease (MD) were studied. For differential diagnosis from other oncogenic viral infections immunohistochemistry as an important tool was investigated.&nbsp;<br />
<br />
<strong>Methods:</strong> The conventional diagnosis of MD) was done on basis of clinical signs, gross lesions and histopathology of proliferate lesions; as lymphomatous lesions consist of pleomorphic lymphocytes. For confirmatory diagnosis molecular techniques were employed to detect MD virus specific Meq gene by Taqman probe real time PCR and nested PCR. For differential diagnosis of mixed oncogenic viral infections, immunohistochemistry (IHC) using B and T cell differentiation markers (CD79 alpha and CD3) was employed.<br />
<br />
<strong>Result:</strong> Specific Meq gene by Taqman probe real time PCR was positive in 92.5% suspected cases; while nested PCR technique gave positivity in 55% cases. The immunohistochemical reactivity for CD3 gave positive reactivity in the cytoplasm and cell membrane and negative reaction for CD79 alpha in MD positive cases, while in cases with mixed infections of MDV and other viral neoplastic conditions, cells showed positive reactivity to both CD3 and CD79 alpha in neoplastic proliferates.&nbsp;</div>


<p>Molecular and Immunopathological Investigations of Marek&rsquo;s Disease in HVT Vaccinated Chicken Flocks</p>


<div style="text-align: justify;">The distrubition of lymph nodes located in the abdominal and thoracic cavities of ten wild boars, and their structure were determined anatomically, histologically and immunohistochemically, to be the first detailed investigation on the wild boar. Though general localization and distribution were similar, the number of lymph nodes showed small differences from those of domestic pig. Histological investigations did not reveal a significant hilus. Besides, T lymphocytes with anti-CD3, CD4, CD8, B lymphocytes with anti-CD79a, macrophages with anti-macrophage monoclonal antibodies, and follicular dendritic cells using anti-S100 polyclonal antibody and their distrubution in the lymph nodes were detected. Many CD3 positive T lymphocytes were observed in the germinal center of the lymph follicles, in the cortical area and in the medulla. CD8 positive T lymphocytes were few, and CD4 positive T lymphocytes were not seen. CD79 positive cells were scanty.</div>

Immunohıstochemıcal staınıng of cd3, cd79αcy and s-100 on bursa fabrıcıus, thymus and spleen of turkeys (Meleagrıs gallapavo)

Molecular and Immunopathological Investigations of Marek’s Disease in HVT Vaccinated Chicken Flocks

The architecture of the lymph nodes in the abdominal and thoracic cavities of wild boar