Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 53 issue 11 (november 2019) : 1450-1454

Immunohıstochemıcal staınıng of cd3, cd79αcy and s-100 on bursa fabrıcıus, thymus and spleen of turkeys (Meleagrıs gallapavo)

Tolunay Kozlu, Ebru Karadağ Sari, Yeşim Akaydin Bozkurt, Nevin Kurtdede
1Department of Histology and Embryology, Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay, Turkey.
Cite article:- Kozlu Tolunay, Sari Karadağ Ebru, Bozkurt Akaydin Yeşim, Kurtdede Nevin (2018). Immunohıstochemıcal staınıng of cd3, cd79αcy and s-100 on bursa fabrıcıus, thymus and spleen of turkeys (Meleagrıs gallapavo). Indian Journal of Animal Research. 53(11): 1450-1454. doi: 10.18805/ijar.B-863.
Distribution of CD3, CD79ácy and S-100, immunohistochemically, in the bursa of Fabricius, thymus and spleen has not been reported in turkeys. Therefore, we determined the localisation of anti-CD3 protein antibodies for mature T-lymphocytes, anti-CD79ácy antibodies for B-lymphocytes and anti-S-100 protein antibodies for follicular dentritic cells in the bursa of Fabricius, thymus and spleen, which are the lymphoid organs, in a study sample of turkeys. Triple staining method was applied to demonstrate the general structure. Moreover, in all the organs positive reactions were observed with the CD3, CD79ácy and S-100 antibodies. It was also found that similar areas had a positive reaction with CD79ácy and S-100 in all of the tested organs. It was remarkable that CD79ácy reacted positively on Hassall’s corpuscles (strongly) and the reticular cells (weakly) in the medulla of the thymus instead of the B-lymphocyte positive areas. 
  1. Atoji Y, Kato A, Masegi T, Nakaoka T, Suzuki Y. (1991) S-100 immunoreactive cells in the spleen and bursa of Fabricius of broiler chickens. J Comp Pathol. 104(3): 281-8.
  2. Bertram EM, Wilkinson RG, Lee Belinda A, Jilbert Allison R, Kotlarski I. (1996) Identification of duck T lymphocytes using an anti-    human T cell ( CD3) antiserum. Vet Immunol and Immunopathol. 51: 353-363. 
  3. Bucy RP, Coltey M, Chen CI, Char D, Le Douarin NM, Cooper MD. (1989) Cytoplasmic CD3+ surface CD8+ lymphocytes develop as a thymus-independent lineage in chick-quail chimeras. Eur J Immunol. 19(8):1449-55.
  4. Castagna C, Viglietti-Panzica C, Carlo Panzica G. (2003) Protein S-100 immunoreactivity in glial cells and neurons of the Japanese quail brain. J Chem Neuroanat. 25(3):195-212.
  5. Ciriaco E, Píñera PP, Díaz-Esnal B, Laurà R. (2003) Age-related changes in the avian primary lymphoid organs (thymus and bursa of Fabricius). Microsc Res Tech. 62(6): 482-7.
  6. Crossmon G. (1937) A modification of Mallory’s connective tissue stain with a discussion of the principles involved. Anat Rec. 69: 33-38.
  7. David CW, Norrman J, Hammon HM, Davis WC, Blum JW. (2003) Cell Proliferation, Apoptosis, and B- and T-Lymphocytes In: Peyer’s Patches of the Ileum, in thymus and in lymph nodes of preterm calves, and in full-term calves at birth and on day 5 of life. J Dairy. 86(10): 3321-9.
  8. Davidson NJ, Boyd RL. (1992) Delineation of chicken thymocytes by CD3-TCR complex, CD4 and CD8 antigen expression reveals phylogenically conserved and novel thymocyte subsets. Int Immunol. 4(10): 1175-82.
  9. Gallego M, Olah I, Del Cacho E, Glick B. (1993) Anti-S-100 antibody recognizes ellipsoid-associated cells and other dendritic cells in the chicken spleen. Dev Comp Immunol. 1993; 17(1): 77-83.
  10. Gallego M, Del Cacho E, Felices C, Varas A, Bascuas JA. (1996) Distribution of bursal secretory dendritic cells in the chicken. Anat Rec. 246(3): 372-6.
  11. Glick B. (1977) The bursa of Fabricius and immunoglobulin synthesis. Int Rev Cytol. 1977; 48: 345-402.
  12. Girolamo P, Alì S, Pelagalli GV. (2003) Presence of S-100-like protein in non-mammalian vertebrate kidney. An immunocytochemical study. Vet Res Commun. 27 Suppl 1: 591-3.
  13. Hodges RD. (1974) The circulatory system. In: The Histology of the Fowl, Academic Press, London, 151–241.
  14. Hoffmann-Fezer G, Rodt H, Götze D, Thierfelder S. (1977) Anatomical distribution of T and B lymphocytes identified by immunohisto-    chemistry in the chicken spleen. Int Arch Allergy Appl Immunol. 55(1-6):86-95.
  15. Igyártó BZ, Nagy N, Magyar A, Oláh, I. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody. Poult Sci. 87(2): 351-5.
  16. Jeurissen SHM. (1993) The role of various compartments in the chicken spleen during an antigen-specific humoral response. Immunol. 80: 29-33.
  17. John JL. (1994) The avian spleen: a neglected organ. Q Rev Biol. 69(3):327-51. Review.
  18. Karadag Sarý E, Kurtdede N. (2007) Light and Electron Microscopic Studies of the Bursa of Fabricius in Turkeys. Kafkas Üniv Vet Fak Derg. 13 (2): 177-184.
  19. Kasai K, Nakayama A, Ohbayashi M, Nakagawa A, Ito M, Saga S, Asai J. (1995) Immunohistochemical characteristics of chicken spleen ellipsoids using newly established monoclonal antibodies. Cell Tissue Res. 281:135-41. 
  20. Kendall M.D. (1980) Avian thymus glands: a review. Dev Comp Immunol. 4: 191–209.
  21. Kozlu T, Karadag Sarý E, Akaydýn Bozkurt Y, Altunay H. (2011) A comparative study on the histological comparative study on the histological structure of the spleen in the ostrich (struthio camelus), the kestrel (falco týnnunculus) and the osprey (pandion haliaetus). Acta Biol Hung. 62(2): 113–121.
  22. Liman N, Alan E, Beyaz F. (2009) Immunohistochemical demonstration of S-100 protein in the chicken uropygial gland during the post-hatching period. Zool Sci. 26(9): 600-7.
  23. Nickel R, Schummer A, Seiferl E. (1977) Anatomy of the Domestic Birds. 4th Ed. S.S. Verlag Paul Parey, Berlin, Hamburg, pp: 56, 60,107. 
  24. Olah I, Glick B. (1982) Splenic white pulp and associated vascular channels in chicken spleen. Am J Anat. 165:445–480.
  25. Olah I, Glick B. (1992) Follicle-associated epithelium and medullary epithelial tissue of the bursa of Fabricius are two different compartments. Anat Rec. 233: 577-587.
  26. Oláh I, Vervelde L. (2008) Avian Immunology: structure of the avian lymphoid system. Chapter 2, 13-15. 
  27. Olah I, Nagy N, Magyar A, Palya V. (2003) Immunology and Molecular Biology, Esophageal Tonsil: A Novel Gut-Associated Lymphoid Organ. Poul Sci. 82: 767–770.
  28. Onyeanusi JC, Ema AN. (1993) The anatomy of the cloacal bursa (Bursa of Fabricius) in the helmeted guinea fowl (Numida meleagris galeata). Anat Histol Embryol. 22: 212-221.
  29. Powers LV. (2000) The Avian Spleen: Anatomy, Physiology, and Diagnostics Compendium on Continuing Education for the Practising Veterinarian-North American Edition. 22:838-843. 
  30. Sayegh CE, Demaries SL, Pike KA, Friedman JE, Ratcliffe MJH. (2000) The chicken B-cell receptor complex and its role in avian B-    cell development. Immunol Rev. 175: 187–200. 

Editorial Board

View all (0)