<div>This study presents an interface, &lsquo;Climate Change Trend Analysis (CCTA)&rsquo;, developed in MATLAB&reg; environment to analyze the trends using non-parametric statistical methods, Mann-Kendall (MK) test and modified Mann-Kendall (MMK) test with Sen&rsquo;s slope estimator. The interface was used to determine trend in annual and seasonal (kharif) rainfall depths in Pune district acquired from 13 observatories. The developed interface automates the trend analysis process, which can further use for detecting variability and trends in the meteorological as well as other hydrological and agricultural parameters. The observed rainfall trends during monsoon would play a significant role for rainfed agriculture in Pune district.&nbsp;</div>


<div>Development of an Interface in MATLAB for Trend Analysis of Hydro-Meteorological Data</div>


<div>Clostridium chauvoei, an anaerobic bacterium reported worldwide, is responsible for Black Quarter, a dreadful disease of ruminants. This bacterium produces many toxins responsible for the pathogenesis of the disease. Except for the well-studied virulence factors such as cctA, flagellin andsialidase genes, the exact role of other toxins of C. chauvoei remains unknown. This necessitates studies on the activities of the C. chauvoei toxins and virulence. In the present study, Twin-Arginine Translocation D (TatD) family deoxyribonuclease of the bacterium was selected. The tatD gene C. chauvoei was amplified by PCR and cloned into p-Rham-N-His-SUMO-Kan expression vector, followed by transformation into the E.cloni 10G competent cells. Clones obtained were confirmed by colony PCR. These tatDclones were sequenced and analysed phylogenetically, which revealed the close relationship of C. chauvoei strain to C. isatidis, C. saccharobutylicum, C. botulinum and C. taeniosporum based on tatD sequence analysis. Upon induction of the clones with L-rhamnose,the protein expression was obtained at 42.3 kDa and the same was further confirmed by Western blotting.</div>


Development of an Interface in MATLAB for Trend Analysis of Hydro-Meteorological Data

Cloning and Expression of Twin-Arginine Translocation D Family Deoxyribonuclease of Clostridium Chauvoei