<div>Background: The work reported in this paper targets the ethnobotanical role of one of the traditional medicinal plant Solanum xanthocarpum, whose medicinal value was reported in Ayurveda. Traditionally Solanum xanthocarpum was used as the antidote for treatment of snake bites by different tribal community of India, to explore and scientifically validate these concept this work was planned. 
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Methods: We have prepared the hydro ethanolic root extract (HERE) of Solanum xanthocarpum and investigated its pharmacological activity both in vitro and in vivo, targeting the enzymes present in Naja snake venom. We have used the wistar albino rat as the animal model in our study.&nbsp; 
 
Result: It was inferred by our study that Solanum xanthocarpum is safe for wistar albino rats upto 2000 mg/kg body weight. Phospholipase A2 (PLA2) and anti-fibrinogenolytic activity are significantly neutralized by Solanum xanthocarpum. Dose dependent inhibition of human red blood cell (HRBC) lysis was also observed. In the future, bioactive compounds from HERE could be isolated and standardized to act as an antidote to Naja venom.</div>

Antivenom Activity of Solanum xanthocarpum Hydroethanolic Root Extract Targeting the Enzymes Present in Naja Snake Venom

<div>Background: Many plants are used to cure snakebites in Indian traditional medicine and some of them have been examined for their ability to neutralize snake venom. The main aim of the present study is the screening of phytochemicals, in vitro assessment of venom toxicity and neutralization assays and its antimicrobial assay. 
 
Methods: The phytochemical screening and thin-layer chromatography was used to determine and separate the chemical constituents present in the Calotropis gigantea. The direct hemolysis using sheep RBCs was studied for Echis carinatus venoms. The proteolytic, neutralizing and antimicrobial activity of the extract was also studied.&nbsp; 
 
Result: The phytochemical investigation of root extract of Calotropis gigantean and the TLC was performed. The Protein concentration of the lyophilized snake venoms was found to be 0.692 mg/ml. Echis carinatus venoms showed 89% hemolysis. Echis carinatus venom produced a reduced zone of hydrolysis with the extract. The absence of clot formation showed the neutralizing ability of plant extracts in gelatin liquefaction assay. Thus the scientific confirmation of neutralization potential of Calotropis gigantea plant extract against Saw scaled viper (Echis carinatus) venom was performed.</div>

Antivenom Activity of Solanum xanthocarpum Hydroethanolic Root Extract Targeting the Enzymes Present in Naja Snake Venom

Bioactive Potential of Aqueous Extract of Calotropis gigantea against Echis carinatus Venom