<div>Background:&nbsp;The adoption of agricultural technology contributes a vital role in increasing the use and profitability of agricultural resources.Strategic commodities, such as onions, should receive special attention in technology development to increase production to support the Indonesian economy. This study aimed to analyze the effectiveness of using AMOS in discussing technology adoption in onion cultivation. 
 
Methods:&nbsp;The research method was carried out using the Structural Equation Modeling (SEM) approach, with the solution using the Analysis of Moment Structures (AMOS).The research was conducted in the Provinces of South Sulawesi and East Java, focusing on Enrekang Regency, South Sulawesi Province and Nganjuk Regency, East Java Province, each covering two sub-districts, resulting in a total of four sub-districts. 
 
Result: The results showed that onion farmers in South Sulawesi and East Java were relatively more adaptive to adopting agricultural technology, as indicated by the hypothesis testing results.The CR values for H1 was 0.043 and H2 -0.041.</div>

Enhancing Onion Cultivation in Indonesia: AMOS Analysis of Technology Adoption in South Sulawesi and East Java

Background: Brucellosis is an important zoonotic disease in several parts of the world including India. The current study was undertaken for molecular characterization and PCR-based detection of Brucella species of cattle from Maharashtra, India. 
 
Methods: A total of 220 clinical samples (vaginal swabs and placental tissues) were collected from cattle of various organized and unorganized farms from different regions of Maharashtra. These samples were processed for isolation of Brucella organisms and further characterized by PCR and sequencing. 
 
Result: A total of 10 Brucella isolates were recovered from 220 samples (5.54%). BCSP 31&nbsp; and 16S rRNA PCR showed an amplicon of 223 and 1412 bp in 10 isolates respectively. Species identification was performed using IS711 PCR, conventional methodology, Bruce-ladder PCR and AMOS PCR. During the study period, a total of ten isolates from vaginal swab and placental tissues of cattle were identified as Brucella abortus. The phylogenetic analysis detected the close relationship of the organism with that of isolate from Italy. Correct and timely diagnosis of brucellosis is important for control of&nbsp; disease. The molecular methods used in the current study aided to speed up the diagnosis of the disease. 
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Molecular Characterization and PCR-based Detection of Brucella abortus Clinical Isolates from Cattle in Maharashtra, India

Background: Brucellosis is one of the threads to the global world and also one of the major zoonotic problem for both humans and animals worldwide. Brucella is the gram negative bacteria which produce Brucellosis and having clinical characteristics like mastitis, repeat breeding, retention of placenta, abortion in the last trimester and also there will be reduction in milk in dairy cattle. Male also shows some clinical characteristics like epididymitis, orchitis and sterility. The disease can affect Humans and can also exhibit few symptoms like undulant fever, arthritis and orchitis etc. 
 
Methods: A total of 405 serum samples were collected comprising dairy Cattle (female-372 and male-33) from various blocks of Mathura district. Samples were process from detection of Brucellosis by RBPT, STAT, ELISA, MRT and 5 tissue samples were subjected to molecular analysis by PCR for the disease. 
 
Result: Based on the analysis, out of total 405 animal samples, Dairy cattle animals showed 49 (13.17%) positivity with RBPT. STAT showed 43 (11.55%) in dairy cattle. Similarly, I-ELISA revealed 55 (14.78%). Further, C-ELISA revealed 42 (93.34%) in dairy cattle. PCR detected amplicons of 223 bp in tissue samples (n=3), employed whereas only one were detected with B. melitensis and B. ovis, where as 2 were detected for B. abortus and B. suis by AMOS PCR at 498 bp, 285 bp, 976 bp and 731 bp respectively.

Enhancing Onion Cultivation in Indonesia: AMOS Analysis of Technology Adoption in South Sulawesi and East Java

Molecular Characterization and PCR-based Detection of Brucella abortus Clinical Isolates from Cattle in Maharashtra, India

Comparative Efficacy of Serological Tests and Molecular Analysis of Brucellosis in Dairy Cattle of Mathura District