Physical characteristics of several superior soybean varieties
Morphological characterization shows a wide variety of soybean trichome colors, with the following distribution: brown (47.62%), brownish white (19.05%), white (14.29%), light brown (9.52%), dark brown (4.76%) and dark green (4.76%). Leaf morphology shows an oval configuration (52.38%), a slightly rounded shape (25%), a pointed shape (7.62%), an elongated oval structure (5%), a triangular shape (5%) and an angular shape (5%). Pod pigmentation is brown (38.09%), pale brown (23.81%), Gepak Kuning (19.05%), dark brown (14.29%) and yellowish brown (4.76%), as presented in Table 1. Seed size is large (57.14%) and medium (42.86%).
Semahu and Purwanto (2016) showed that Grobogan has a substantial seed size (19.00 g), while Anjasmoro has a moderate seed proportion (11.00 g).
Sinta and Sugito (2020) showed that Dena-1, Dega-1 and Grobogan have substantial seed sizes with a 100-seed weight of 23.86 g, 22.76 g and 20.66 g, respectively. Furthermore, findings by
Sirait and Karyawati (2019) confirmed that the Dena-1 and Grobogan cultivars have substantial seed sizes, at 19.80 g and 19.79 g, respectively. Seed size, measured by 100-seed weight, is positively correlated with seed yield.
Saikia et al. (2025) found that seed size or 100-seed weight correlated significantly with mung bean seed yield (0.603%).
Leaf shape
The Derap-1 cultivar exhibits a slightly rounded leaf shape (Fig 1a), while the Gepak Kuning cultivar exhibits an oblong leaf shape (Fig 1b). The oblong leaf morphology characterizes several varieties, including Deja-1, Anjasmoro, Dena-1, Dega-1, Deja-1 and Demas-1 (Table 1). A slightly rounded leaf shape is found in Detap-1 (Fig 1c). In addition to the Detap-1 cultivar, this slightly circular leaf pattern also appears in the Devon-1 and Devon-2 varieties. The pointed leaf configuration is characteristic of the Grobogan variety (Fig 1d). Research by
Fattah et al. (2024) confirmed that the slightly rounded leaf shape characterizes the Devon-2 and Devon-1 cultivars, while the pointed leaf structure is characteristic of the Grobogan variety. Leaf morphology shows a positive correlation with chlorophyll concentration, which plays an important role in photosynthetic performance and productivity potential
(Sakoda et al., 2016; Yu et al., 2020).
The color of the pods
Pod coloration varies among cultivars, displaying yellow pigmentation (Derap-1), deep brown hues (Deja-1), brown tones (Grobogan) and pale brown shades (Anjasmoro) (Fig 2). Soybean cultivars exhibiting brown-colored pods demonstrate correlation with enhanced grain quality, as elevated protein concentrations and lignin deposits within the testa and pod structures provide enhanced mechanical durability, resulting in superior physiological integrity and pathogen resistance in seeds
(Krzyzanowski et al., 2023).
Length, number and shape of trichomes on soybean pod and leaves
Maximum foliar trichome dimensions were observed in Grobogan (30.93 mm), Demas-1 (27.86 mm), Derap-1 (25.73 mm) and Deja-2 (25.28 mm), whereas minimal measurements were recorded in Anjasmoro (21.60 mm). The greatest foliar trichome density occurred in Devon-2 (71.00 units), Detap-1 (57.27 units) and Dega-1 (56.67 units), while the lowest density was documented in Deja-1 (27.13 units) (Table 2) and (Fig 3).
Maximum pod trichome dimensions were recorded in Detap-1 (46.07 mm), whereas minimal lengths were observed in Demas-1 (26.52 mm), Grobogan (26.96 mm), Dena-2 (27.25 mm) and Dega-2 (27.40 mm). Peak pod trichome abundance was documented in Derap-1 (300.00 units) and Dega-1 (247.60 units), while the most limited trichome counts occurred in Detap-1 (126.20 units), Anjasmoro (132.67 units) and Gepak Kuning (132.33 units) (Table 2).
Microscopic examination revealed substantial inter-varietal differences regarding trichome density and dimensions across both foliar and pod tissues (Fig 3 and 4). The cultivars Devon-2, Dena-2 and Derap-1 demonstrated unique trichome attributes in terms of both dimensional characteristics and abundance, suggesting enhanced inherent defense mechanisms against arthropod colonization. Research by
Murgianto et al. (2023) documented that Devon-2 (foliar tissue) and Derap-1 (pod surface) soybean cultivars displayed maximum trichome concentrations, which showed strong correlation with substantial decreases in whitefly oviposition and larval populations (R² = 0.84). Extended trichome structures can amplify the mechanical barrier effects against herbivorous insects (
Adie and Krisnawati, 2017). Trichome density and dimensions substantially impede arthropod behavior through dual mechanisms involving mechanical obstruction and secretion of bioactive secondary metabolites
(Abdelhamid et al., 2024; Fattah et al., 2024).
Elevated trichome density demonstrates positive correlation with enhanced arthropod resistance, as these structures create physical impediments to pest colonization (
Adie and Krisnawati, 2017). Additionally, elongated pod trichomes reportedly restrict pest infiltration, offering distinctive mechanical protection despite reduced overall abundance. In contrast, Detap-1 and Anjasmoro cultivars, characterized by diminished trichome populations, exhibit greater vulnerability to arthropod infestation
(Abdelhamid et al., 2024).
Insect populations and the level of damage caused to soybean leaves and pods
One type of pest that damages soybean leaves is
O.servile (Fig 5a) with the highest population density found in Devon-2, Dena-2, Grobogan, Devon-1, and Dega-1, while the symptoms of soybean leaf damage due to O. servile attacks (Fig 5b) were lowest found in Grobogan and Dega-1. The type of pest that damages soybean pods the most is
R.linearis (Fig 5c) with the lowest population found in Derap-1, Dena-1, Dena-2, Grobogan, and Devon-1. Meanwhile, the lowest level of pod damage caused by
R. linearis was found in Devon-2, Dena-2, Derap-1 and Detap-1. Furthermore, the lowest population of
P. inclusa was found in Devon-2, Dena-2 and Detap-1 (Table 3). The highest populations of
R. linearis,
P. inclusa and
O. servile were found in Anjasmoro, Deja-2 and Demas-1. Elevated population densities showed positive correlation with intensified tissue damage severity due to pest preference for more tender plant structures or specific secondary metabolite profiles
(Coolen et al., 2024). Tender plant tissues typically exhibit greater palatability, enhancing their attractiveness to herbivorous arthropods
(Souza et al., 2013). Moreover,
Al-Khayri et al. (2023) determined that softer tissues combined with reduced secondary metabolite concentrations can increase pest attraction.
Cultivar resistance mechanisms are intimately linked to plant morphological attributes including trichome abundance, cuticular thickness and concentrations of defensive compounds such as flavonoids or glucosinolates
(Xing et al., 2017). Additionally, these researchers demonstrated that foliar trichome density serves as a mechanical deterrent against pest infiltration and attachment, while simultaneously reducing herbivorous insect feeding behavior. Arthropod resistance results from combined trichome characteristics and genetic expression pathways controlling terpene, flavonoid and phenyl propanoid synthesis, along with pathogen defense mechanisms (
Dixon and Gschwend, 2024).
The assessment of pod deterioration caused by
R. linearis (Fig 5c) infestation revealed substantial varietal differences in susceptibility patterns across the tested soybean cultivars. Among the superior performing varieties, minimal damage levels were consistently observed in Devon-1 (3.15%), Dena-2 (3.17%), Detap-1 (3.45%), Derap-1 (3.47%) and Grobogan (3.67%), indicating these cultivars possess inherent resistance mechanisms that effectively limit hemipteran colonization.
In stark contrast, several cultivars exhibited significantly elevated vulnerability to
R. linearis attacks, with Deja-2 (12.16%) and Anjasmoro (12.78%) showing particularly severe damage levels (Fig 5d), while Gepak Kuning also demonstrated high susceptibility. The nearly four-fold difference in damage intensity between resistant and susceptible varieties underscores the critical importance of varietal selection in integrated pest management programs. Supporting evidence from previous research reinforces these observations, as
Fattah et al. (2021) documented similar patterns of differential
R. linearis colonization across soybean varieties, though with generally higher damage levels than observed in the current study. Their findings showed reduced infestation rates in Grobogan (8.50%), Argomulyo (9.40%) and Detam-1 (9.0%), suggesting that environmental conditions and management practices may influence the absolute magnitude of pest damage while maintaining relative resistance rankings among cultivars. The morphological basis for these resistance differences has been elucidated by
Sarjan and Sab’i (2014), who established strong correlations between pericarp thickness and
R. linearis infestation intensity, indicating that physical pod characteristics serve as primary defense mechanisms against hemipteran penetration.
The temporal dynamics of pest-plant interactions add another layer of complexity to
R. linearis management strategies. Research by
Defensor et al. (2020) demonstrated that soybean developmental stages significantly influence hemipteran population dynamics, with critical vulnerability periods occurring during anthesis, reproductive development and physiological maturity phases. This phenological synchronization between pest activity and plant development suggests that resistant varieties may possess temporal advantages in addition to their morphological defense mechanisms, potentially offering enhanced protection during the most susceptible growth stages when pod formation and seed development are most critical for final yield determination. In addition to physical factors, varieties also influence pest resistance. According to
Sathish et al. (2024), biochemical content, such as flavonoids and phenols, is negatively correlated with the level of seed damage caused by Callosobruchus chenensis attacks (r = -0.752 and r = 0.729) and high phenol content makes plants resistant to pod borer attacks.
Maximum grain productivity was recorded in the Dena-2 cultivar (3.78 t ha
-1), whereas minimal yields occurred in Anjasmoro (1.93 t ha
-1) and Deja-2 (2.02 t ha
-1), while Grobogan demonstrated intermediate productivity levels of approximately 2.96 t ha
-1 (Table 3). The Dena-2 cultivar exhibits multiple beneficial characteristics, including superior grain productivity, tolerance to hemipteran pod pests and resistance to lepidopteran defoliator
S. litura.
Economic value of each variety
The commercial potential of individual soybean cultivars demonstrates significant variation based on grain dimensions. Multiple cultivars possess substantial seed size combined with elevated productivity, encompassing Derap-1, Devon-2, Detap-1, Dega-1 and Grobogan. Cultivars characterized by larger grain dimensions command superior market value compared to those with smaller seed characteristics. Large-seeded cultivars receive preference from tempeh manufacturers over their smaller counterparts. Research by
Ginting et al. (2009) established that grain dimensions determine tempeh product quality through positive correlation with final product mass and volume. Large-seeded soybean cultivars demonstrate predominant utilization in soymilk production relative to small-seeded varieties
(Ginting et al., 2009).