Nickel Fertilization as Plant’s Foes or Friends?- Evaluation by Yield Attributes of Mash [Vigna mungo (L.) Hepper] Genotypes

DOI: 10.18805/LRF-677    | Article Id: LRF-677 | Page : 872-877
Citation :- Nickel Fertilization as Plant’s Foes or Friends?- Evaluation by Yield Attributes of Mash [Vigna mungo (L.) Hepper] Genotypes.Legume Research.2022.(45):872-877
Ghulam Yasin, Saira Sameen, Ikram ul Haq, Shahzadi Saima, Adeela Altaf, Aleem A. Khan yasingmn_bzu@yahoo.com
Address : Department of Botany, Bahauddin Zakariya University, Multan. Pakistan.
Submitted Date : 31-12-2021
Accepted Date : 20-03-2022


Background: Nickel can act as micronutrient essential for nitrogen fixation in legume crops or it can cause toxicity when present in high concentration. Rhizospheric supplement of Nickel be at a dose not beyond its beneficial level. 
Methods: An experiment was conducted for mash bean genotypes to evaluate the toxic level of Nickel concentrations. Seeds of four genotypes were sown in earthen pots filled with homogenized loamy soil. Nickel was added as its chloride salt solutions at the age of twenty days @ 15.0, 30.0, 45.0, 60.0, 75.0 and 90.0 mg kg-1 soil. Yield plant-1 and its contributing factors were recorded at physiological maturity of crop. 
Result: At low concentration, Nickel appeared to be non toxic and high doses reduced yield attributes. The lowest significantly effective dose which affected the parameters was 45 mg kg-1 except for number of legumes plant-1 for which same was true at 60 mg kg-1. While, the most effective dose was 45 mg kg-1 for each attributes. The observations were excluded from the ongoing trend when 15 mg kg-1 Nickel reflected positive role. Of the genotypes, MASH 80 was the least productive while MASH 88 was the most productive. In terms of grain numbers, MASH 97 was the least sensitive.


Genotypes Grain weight Mash Nickel Yield


  1. Aendo, P., Netvichian, R., Khaodhiar, S., Thongyuan, S., Songserm, T., Tulayakulm, P. (2020). Pb, Cd and Cu play a major role in health risk from contamination in duck meat and offal for food production in Thailand. Biology of Trace Elements Research. 1-10.
  2. Ahmad, M.S.A., Hussain, M., Saddiq, R., Alvi, A.K. (2007). Mungbean: A nickel indicator, accumulator or excluder? Bulletin of Environmental Contamination and Toxicology. 78: 319- 324. 
  3. Ali, H. and Khan, E. (2019). Trophic transfer, bioaccumulation and biomagnification of non-essential hazardous heavy metals and metalloids in food chains/webs-concepts and implications for wildlife and human health. Human and Ecological Risk Assessment: An International Journal. 25: 1353-1376. 
  4. Andrews, M., Sprent, J.I., Raven, J.A., Eady, P.E. (1999). Relationship between shoot to root ratio, growth and leaf soluble protein concentration of Pisum sativum, Phaseolus vulgaris and Triticum aestivum under different nutrient deficiencies. Plant Cell and Environment. 22: 949-958.
  5. Arun, K.S., Cervantes, C., Loza-Tavera, H., Avudainayagam, S.  (2005). Chromium toxicity in plants. Environmental International. 31: 739-753.
  6. Asad, S.A., Farooq, M., Afzal, (2019). Integrated phytobial heavy metals remediation strategies for sustainable clean environment-A review. Chemosphere. 217: 925-941.
  7. Balaguer, J. et al. (1998). Tomato growth and yield affected by nickel presented in the nutrient solution. Acta Horticulturae. 458: 269- 272.
  8. Biswas, S., Banerjee, R., Bhattacharyya, D., Patra, G., Das, A.K., Das, S.K. (2019). Technological investigation into duck meat and its products-a potential alternative to chicken. World Poultary Science Journal. 75: 609-620.
  9. Brun, W.A. and Betts, K.J. (1984). Source/sink relations of abscising and non abscising soybean flowers. Plant Physiology. 75: 187-191. 
  10. Budijono, M.H., Purwanto, E., Eddiwan, K., Siregar, B.Y. (2017). The phytoremediation of Pb and Zn in the Siak River by Ceratophyllum demersum. International Journal of Science Research. 6: 1522–1525.
  11. Bueno, M.S., Alonso, A., Villalobos, N. (1994). Nitrate reduction in cotyledons of Cicer arietinum L., regulatory role of cytokinins. Plant Sciences. 95: 117-124.
  12. Collier, R. and Tegeder, M. (2012). Soybean ureide transporters play a critical role in nodule development, function and nitrogen export: Nitrogen transport in soybean nodules. Plant Journal. 72: 355-367.
  13. Dalir, N. and Khoshgoftarmanesh, A.H. (2015). Root uptake and translocation of nickel in wheat as affected by histidine. Journal of Plant Physiology. 184: 8-14.
  14. Das, K.K., Das, S.N., Dhundasi, S.A. (2008). Nickel, its adverse health effects and oxidative stress. Indian Journal of Medical Research. 128: 412.
  15. Duncan, D.B. (1955). Multiple Range and Multiple F-Test. Biometrics. 11: 1-42.
  16. Eskew, D.L., Welch, R.M., Cary, E.E. (1983). Nickel: an essential micronutrient for legumes and possibly all higher plants. Science. 222: 621-623. 
  17. Fabiano, C., Tezotto, T., Favarin, J.L., Polacco, J.C., Mazzafera, P. (2015). Essentiality of nickel in plants: A role in plant stresses. Frontier in Plant Sciences. 6: 754.
  18. Fargasova, A. (2001). Phytotoxic effects of Cd, Zn, Pb, Cu and Fe on Sinapis alba L. seedling and their accumulation in roots and shoots. Biologia Plantarum. 44: 471-473.
  19. Georgiadou, E.C. et al. (2018). Influence of heavy metals (Ni, Cu and Zn) on nitro-oxidative stress responses, proteome regulation and allergen production in basil (Ocimum basilicum L.) plants. Frontier in Plant Sciences. 9: 862.
  20. González-Guerrero, M., Matthiadis, A., Saez, A., Long, T.A. (2014). Fixating on metals: New insights into the role of metals in nodulation and symbiotic nitrogen fixation. Frontier in Plant Sciences. 13: 5-45.
  21. Hayati, R.D., Egli, B., Crafts-Brandner, S.J. (1995). Carbon and nitrogen supply during seed ®lling and leaf senescence in soybean. Crop Sciences. 35: 1063-1069.
  22. Hosseini, H. and Khoshgoftarmanesh, A.H. (2013). The effect of foliar application of nickel in the mineral form and urea- Ni complex on fresh weight and nitrogen metabolism of lettuce. Science of Horticulture. 164: 178-182.
  23. Jose, I.G.P., Miguel, P.E., Beatriz, F., Astrid, K., Ulo, N. (2017). Emissions of carotenoid cleavage products upon heat shock and mechanical wounding from a foliose lichen. Environmental and Experimental Botany. 133: 87-97.
  24. Khoshgoftarmanesh, A.H., Hosseini, F., Afyuni, M. (2011). Nickel supplementation effect on the growth, urease activity and urea and nitrate concentrations in lettuce supplied with different nitrogen sources. Science of Horticulture. 130: 381-385. 
  25. Kumar, S., Kumar, A., Jamwal, S., Abrol, V., Singh, A.P., Singh, B., Kumar, J. (2021). Divergence studies of blackgram (Vigna mungo L) for selection of drought tolerant genotypes under rainfed conditions of North Western Himalayas in J and K, India. Legume Research. 44: 158-163. 
  26. Kutman, B.Y., Kutman, U.B., Cakmak, I. (2013). Nickel-enriched seed and externally supplied nickel improve growth and alleviate foliar urea damage in soybean. Plant and Soil. 363: 61-75. 
  27. Kutman, B.Y., Kutman, U.B., Cakmak, I. (2014). Effects of seed nickel reserves or externally supplied nickel on the growth, nitrogen metabolites and nitrogen use efficiency of urea- or nitrate-fed soybean. Plant and Soil. 376: 261-276.
  28. Macedo, F.G., Bresolin, J.D., Santos, E.F., Furlan, F., Lopes da Silva, W.T., Polacco, J.C., et al. (2016). Nickel availability in soil as influenced by liming and its role in soybean nitrogen metabolism. Frontier in Plant Sciences. 7: 1-12.
  29. Malavolta, E. Moraes, M. (2007). Nickel - from toxic to essential nutrient. Better Crops. 91: 26-27. 
  30. Maleki, M., Ghorbanpour, M., Kariman, K. (2017). Physiological and antioxidative responses of medicinal plants exposed to heavy metals stress. Plant Genetic. 11: 247-254.
  31. Mohanlal, V.A. Saravanan,K. Sabesan, T. (2021). Water-stress screening in blackgram [Vigna mungo (L.) Hepper] genotypes using Polyethylene Glycol 6000 at seedling growth stage. Indian Journal of Agricultural Research. 55: 440-445.
  32. Pietrini, F. et al. (2015). Evaluation of nickel tolerance in Amaranthus paniculatus L. plants by measuring photosynthesis, oxidative status, antioxidative response and metal-binding molecule content. Environmental Science and Pollution. 22: 482-494.
  33. Pilipovic, A., Zalesny, R.S., Roncevic, S. (2019). Growth, physiology and phytoextraction potential of poplar and willow established in soils amended with heavy-metal contaminated, dredged river sediments. Journal of Environmental Management. 239: 352-365. 
  34. Priya, L., Arumugam Pillai, M., Shoba, D. (2021). Genetic divergence, variability and correlation studies in black gram [Vigna mungo (L.) Hepper]. Legume Research. 44: 36-40.
  35. Selvam, A. and Wong, J.W.C. (2008). Phytochelationand synthesis and Cadmium uptake by Brassica napus. Environmental Technology. 29: 765-773.
  36. Seshadri, B., Olan, N.S., Naidu, R. (2015). Rhizosphere-induced heavy metal (loid) transformation in relation to bioavailability and remediation. Journal of Soil Science and Plant Nutrition. 15: 524-548.
  37. Sharma, P.R. and Dubey, S. (2005). Lead toxicity in plants. Brazilian Journal of Plant Physiology. 17: 35-52.
  38. Sreekanth, T.V.M., Nagajyothi, P.C., Lee, K.D., Prasad, T.N.V.K.V. (2013). Occurrence, physiological responses and toxicity of nickel in plants. International Journal of Environmental Science andTechnology.10: 1129-1140.
  39. Tan, X. W., Ikeda, H., Oda, M. (2000). Effects of nickel concentration in the nutrient solution on the nitrogen assimilation and growth of tomato seedlings in hydroponic culture supplied with urea or nitrate as the sole nitrogen source. Science of Horticulture. 84: 265-273. 
  40. Tripathy, B.C., Bhatia, B., Mohanty, P. (1981). Inactivation of chloroplast photosynthetic electron transport activity by Ni2+. Biochimica et Biophysica Acta. 638: 217-224
  41. Tuna, A.L., Burun, B., Yokas, I., Coban, E. (2002). The effects of heavy metals on pollen germination and pollen tube length in the tobacco plant. Turkish Journal of Biology. 26: 109-113.
  42. Uruç Parlak, K. (2016). Effect of nickel on growth and biochemical characteristics of wheat (Triticum aestivum L.) seedlings. NJAS -Wagening Journal of Life Science. 76: 1-5.
  43. Xun, E., Zhang, Y., Zhao, J., Guo, J. (2017).Translocation of heavy metals from soils into floral organs and rewards of Cucurbita pepo: Implications for plant reproductive fitness. Ecotoxicology and Environmental Safety. 145: 235-243.
  44. Zrenner, R., Stitt, M., Sonnewald, U., Boldt, R. (2006). Pyrimidine and purine biosynthesis and degradation in plants. Annual Review of Plant Biology. 57: 805-836.

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