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Legume Research, volume 45 issue 9 (september 2022) : 1137-1142

Growth and Macronutrients Status of Mung Bean [Vigna radiata (L.) Wilczek] Grown under Lead (Pb) Stress and Exposed to Foliar Spray of Indole Acetic Acid (IAA)

Shahzadi Saima1, Ghulam Yasin1,*, Ikram ul Haq2, Sana Saleem1, Adeela Altaf3, Aqsa Ahmad1, Sumbal Shahzadi1
1Department of Botany, Bahauddin Zakariya University, Multan, Pakistan.
2Institute of Biotechnology and Genetic Engineering, Sindh University, Jamshoro, Pakistan.
3Department of Environmental Sciences, Bahauddin Zakariya University, Multan, Pakistan.

  • Submitted02-12-2021|

  • Accepted26-05-2022|

  • First Online 27-06-2022|

  • doi 10.18805/LRF-670

Cite article:- Saima Shahzadi, Yasin Ghulam, Haq ul Ikram, Saleem Sana, Altaf Adeela, Ahmad Aqsa, Shahzadi Sumbal (2022). Growth and Macronutrients Status of Mung Bean [Vigna radiata (L.) Wilczek] Grown under Lead (Pb) Stress and Exposed to Foliar Spray of Indole Acetic Acid (IAA) . Legume Research. 45(9): 1137-1142. doi: 10.18805/LRF-670.

ABSTRACT

Background: Growth and nutritional status of plant are affected adversely by heavy metals while improved by plant growth regulators. The experiment was aimed to explore the ameliorative potential of indole acetic acid (IAA) for toxicity of Pb. 

Methods: Two varieties i.e., M-8 and MN-92 of mung bean were grown in pots arranged under complete randomization. Fifteen days after germination, Pb was added @ 10 and 20 mg/kg soil as solutions of PbNO3. Indole acetic acid @100.0 mM was sprayed twice at 15 and 30 days of age. Stem length, root length, leaf area and nutrient ions were recorded at physiological maturity. Three replicates from each treatment were evaluated. 

Result: The alleviation role of IAA for stem length was recorded as 21.46 and 26.14% for low and high lead stress. The root length was compensated to 34.78 and 31.40% for the respective low and high lead toxicity. Leaf area was affected as 18.11 and 17.32% by IAA. Similarly, Nitrogen contents were nullified to the extents of 15.34 and 8.13%. Phosphorus contents were changed as 11.62 and 4.65% while potassium contents were as 10.14 and 19.36%. The rectifying potential of IAA for leaf biomasses were as 7.64 and 7.43%.

INTRODUCTION

Rapidly growing trend of urbanization accompanied with more industrialization, has increased the heavy metals in the environment (Ashraf et al., 2019). The effects of heavy metals toxicity are increasing worldwide particularly due to anthropogenic causes. Among the heavy metals pollution soil contamination is one of the most important as it enters into the food chain through plants. During the course of evolution, some plants have developed mechanisms to overcome such stresses. However, metals like Zn, Al, Cu, Pb, As, Cd exert severe effects on plants (Angulo-Bejarano et al., 2021).
       
Heavy metals pollution in the environment is a threatening problem for life of all livings (Heckathorn et al., 2004). Heavy metals of environment finally the food chains and cause stunted growth of plants (Moustaka et al., 2016). Some remedial actions are mandatory to check the emission of heavy metals and their incorporation into various ecosystems (Hasan et al., 2019). These measures can be of physical, chemical or of mechanical types (Wuana and Okieimen, 2011).
       
Mining activities, municipal sewage sludge are the main sources of Pb (Kumar et al., 1995). Lead being the nonessential element for plants accumulates in cell causing interruptions in physiological phenomenon. In plants, Pb toxicity disrupts many metabolic activities like organelles stability, electron transport chain, membrane integrity, photosystems complex, mineral metabolism, enzymatic activity and oxygen-evolving complex (Aslam et al., 2021). Exogenous Pb causes increases leaf H2O2, lipid peroxidation, growth reduction, pigments reduction and photosynthesis decline due to oxidative stress (Giannakoula et al., 2021).
       
Phytohormones, also called as Plant Growth Regulators, are biological molecules which are synthesized within plants and can act at the site of production or are transported to distant cells to promote growth and developmental process in normal and stressed plants (Iqbal et al., 2014). In many countries like EU, USA and Japan PGRs are commercially marketed (Jahan et al., 2019). Exogenous application of several PGRs including auxin promotes growth also (Khan et al., 2004). Exogenous PGRs can mitigate the adverse effects of stress (Bielach et al., 2017). Of these PGRs, auxin is one of the most common. Indole-3-acetic acid (IAA), is the first identified auxin (Fahad et al., 2015). IAA is produced by many plants, yeast (Bunsangiam et al., 2021) and Escherichia coli (Wu et al., 2021). Auxins act as an active signaling molecule (Weijers and Wagner, 2016). Indole-3-acetic acid (IAA) mitigates the adverse effects of stress (Abdel Latef et al., 2021).
       
Mung bean [Vigna radiata (L.) R.Wilczek[ originated in 1,500 BC. Its origin was in subcontinent and thereafter spread to Asian, African, Australian and American countries. Its cultivation extended later on to Himalayas at elevations of 1,850 m (Lambrides and Godwin, 2006). In Pakistan, Mung bean is is cultivated for its use as fodder and green manure (Rehman et al., 2017), as vegetables (AVRDC, 2012) and for improvement of soil fertility due to nitrogen fixing capacity (Ilyas et al., 2018).

MATERIALS AND METHODS

The experiment was for the purpose of finding mitigating capacity of indole acetic acid (IAA) for two mung bean [Vigna radiata (L.) Wickzek] varieties grown in Pb contaminated soil. The experiment was conducted in Bahauddin Zakaryia University, Multan Pakistan during 2016-17.
       
Sandy loam soil never irrigated with effluents was chosen after an initial survey. Pots of plastic material measuring 30 cm diameter were filled with this soil. Two mung bean varieties i.e. M-8 and MN-92 were selected and obtained from pulses department of a research Institute. IAA and Lead nitrate salt of Sigma Aldrich, Japan were acquired from dealer.
       
Soil, after filling of pots, was moisturized with water and left till getting of field capacity moisture contents. Seepage from pots was prevented by lining of pots with polyethylene sheath. Healthy and of uniform size seeds after sorting were sterilized with 1% H2O2. Five seeds in each pots were sown. After germination thinning was carried out to maintain three seedlings per pot to ensure uniform nutrients supply. Normal irrigation and pesticides application were practiced for plant protection. Pots arrangement was by complete randomization of all treatments. According to reviewed literature, Lead stress @10 and 20 mg Pb/kg soil was induced by adding lead nitrate. Pots left without addition of metal salt were as control. IAA (100.0 mM) solution was prepared in water pre-estimated by foliar spray as trial. Surfactant of Tween-20 (0.1%) was mixed with solution. Plants were sprayed after fifteen and thirty days of germination. One group of plants without addition of metal was sprayed with just distilled water.
       
The treatments applied were as: Normal soil (Without addition of metal salts) +Distilled water spray; Normal soil (Without addition of metal salts) + 100.0 mM IAA spray; Lead (10.0 mg/kg soil) + Distilled water spray; Lead (20.0mg/kg soil) + Distilled water spray; Lead (10.0 mg/kg soil) + 100.0 mM IAA spray; Lead  (20.0mg/kg soil) + 100.0 mM IAA spray.
 
Data for three plants as replicates were recorded from every group of treatment. Stem length, root length, leaf number and leaf area were recorded for each. After these recordings respective plants were harvested carefully for NPK determination. NPK were quantified from digested material of plants with the help of spectrophotometer.
       
Using software of COSTAT, results were analyzed at significance level of 5%, Duncan multiple range test was applied for comparison of means (Duncan, 1955). MSTAT-C Computer Statistical Programm was used for significant results to test means by LSD tests.

RESULTS AND DISCUSSION

Stem length (cm)
 
Stem length significantly differed among treatments. Varieties and treatments both revealed highly significant differences. While, interactive response among treatment and varieties were non significantly different (Table 1). IAA increased stem length as 18.61%. Under stress of low and high lead stem length was declined as 38.61 and 46.49% respectively. IAA alleviated the toxic effect of lead, therefore, stem length reduction was limited to 17.15 and 20.34% respectively (Table 2).
 

Table 1: Mean sum of squares for stem length, root length, leaf area, nitrogen contents, phosphorus contents and potassium contents of 40 days age mung bean [Vigna radiata (L.) Wickzek] plants grown in lead (10, 20 mg/kg soil) and exposed to foliar spray of IAA (100 mM)] at 15 and 30 days of age.


 

Table 2: Mean values (± SE) for lead toxicity effect on stem length (cm) of 40 days age mung bean [Vigna radiata (L.) Wickzek] plants grown in lead (10, 20 mg/kg soil) and exposed to foliar spray of IAA (100 mM)] at 15 and 30 days of age.


 
Root length (cm)
 
Highly significant differences were observed in root length by treatments, varieties and also by their interactions (Table 1). IAA increased root length upto 14.46%. Low and high lead decreased root length by 45.96 and 59.45% respectively, IAA mitigated toxic effect of lead. Hence, reduction by metal was 11.18 and 28.05% respectively (Table 3).
 

Table 3: Mean values (±SE) for lead toxicity effect on root length of 40 days age mung bean [Vigna radiata (L.) Wickzek] plants grown in lead (10, 20 mg/kg soil) and exposed to foliar spray of IAA (100 mM) at 15 and 30 days of age.


 
Leaf area (cm2)
 
Results revealed that variety, treatments and their interactions highly significantly different (Table 1). Leaf area was increased by IAA treatment (53.54%). Low and high lead treatments reduced leaf area as 38.61 and 62.20% respectively. IAA alleviated lead stress effects and limited reductions to18.50 and 44.88% respectively (Table 4).
 

Table 4: Means values (±SE) for lead toxicity effect on leaf area of 40 days age mung bean [Vigna radiata (L.) Wickzek] plants grown in lead (10, 20 mg/kg soil) and exposed to foliar spray of IAA (100 mM) at 15 and 30 days of age.


 
Nitrogen (N) contents (mg/g)
 
The data related nitrogen contents showed that highly significant differences were found among different treatments. Varieties and collective response in varieties and treatments showed non-significant differences (Table 1). Increased nitrogen contents (0.09%) were observed by IAA treatment. Low and high lead treatments decreased the nitrogen values 23.25 and 29.96% respectively. IAA ameliorated the lead stress. Therefore, reductions by metal treatments were lowered to 7.90 and 21.83% respectively (Table 5).
 

Table 5: Means values (±SE) for lead toxicity effect on Nitrogen content of 40 days age mung bean [Vigna radiata (L.) Wickzek] plants grown in lead (10, 20 mg/kg soil) and exposed to foliar spray of IAA (100 mM) at 15 and 30 days of age.


 
Phosphorus (P) contents (mg/g)
 
The data revealed that phosphorus contents showed that treatments, varieties and their interactions showed non-significant differences (Table 1). Increased phosphorus contents (6.04%) were observed by IAA treatment. Low and high lead treatments decreased the nitrogen values as 25.11 and 27.90% respectively. IAA ameliorated the lead stress therefore phosphorus was reduced up to 13.48 and 23.23% respectively (Table 6).
 

Table 6: Means values (±SE) for lead toxicity effect on phosphorus of 40 days age mung bean [Vigna radiata (L.) Wickzek] plants grown in lead (10, 20 mg/kg soil) and exposed to foliar spray of IAA (100 mM)] at 15 and 30 days of age.


 
Potassium (K) contents (mg/g)
 
The data related to potassium contents showed that varieties revealed significant differences while treatments and their interactions with varieties treatments showed non-significant differences (Table 1). Increased potassium contents (4.14%) were observed by IAA treatment. Low and high lead treatments decreased the potassium values 12.99 and 26.66% respectively. IAA ameliorated the lead stress therefore metal induced reductions were limited to 2.84 and 6.70% respectively (Table 7).
 

Table 7: Means values (±SE) for lead toxicity effect on potassium of 40 days age mung bean [Vigna radiata (L.) Wickzek] plants grown in lead (10, 20 mg/kg soil) and exposed to foliar spray of IAA (100 mM) at 15 and 30 days of age.


       
Well grown plants of mung bean with adequate amount of nutrients provide more energy in term of food and fodder. Growth was reduced in stem, root and leaves by Pb and enhanced by IAA (Table 2-4). Growth reduction might be owed to a decline of water potential (Atteya, 2002). Water contents of metal stressed plants are lowered by enhanced resistance in its flow (Poschenrieder et al., 1989) or it might be due to metal induced change in cell wall structure (Poschenrieder et al., 1989). Growth and morphology of mung bean is influenced by plant growth promoting rhizobia (PGPR) and other external factors (Neha et al., 2021). Plant growth regulators can influence the productivity of mung bean (Bhadane et al., 2021).
       
Growth reduction might be due to metal induced chlorophyll decrease by denaturation of its biosynthetic enzymes i.e., 6-amino laevulinic acid dehydratase and porphobilinogenase by metal (Hampp et al., 1974).  Lead toxicity affects the rate of photosynthesis (Carlson et al., 1975); changes in the thylakoid structure (Fodor et al., 1996); reduction of mitochondrial cristae (Samardakiewicz, 2000) and phosphorylation process (Wozny, 1995).
       
Reactive oxygen species (ROS) production by metal lowers concentration of osmotica such as carbohydrates and amino acids (Zhang et al., 1999); causes protein and lipid oxidation/ degradation of mitochondrial membrane and inhibition of photosynthetic ETC (Kappus, 1985). Peroxidase reduction by ROS results in shoot growth retardation (Stoeva and Bineva, 2003). However, IAA treated plants showed better expression in all above parameters.
       
Lead exerted a negative impact on N, P, K content in lead treated mung bean plants (Table 5-7). Lead reduces the uptake and transportation of mineral nutrients in plants (Goldbold and Kettner, 1991). Saygideger et al., (2004) observed that high level of Pb could decrease the nitrogen contents in Typha ceratophyllum. The decline in nitrogen concentration due to Pb may be as a result of moisture stress which is created by Pb (Burzynisky and Grabowski, 1984). Application of Pb decreases the nitrogen and phosphorus contents (Kibria et al., 2009). According to Orhue and Innch (2010), concentrations of phosphorous and potassium were significantly decrease in Celosia argentia by treatment of Pb. According to Blatt (1993), transport of ion is regulated by auxin. Auxins cause an increased in concentration of K ion in wheat grain and leaves (Wierzbowska and Bowszys, 2008). Response of the varieties differed to IAA and metal. Yield varies with genetic makeup of mung bean (Priya and Ratna Babu, 2021; Salman et al., 2021)

CONCLUSION

IAA treatment ameliorated the PB toxicity effect to significant extent for growth and to a level of non-significant for ionic concentration. 

CONFLICT OF INTEREST

None.

REFERENCES


  1. Abdel Latef, A.A.H., Akter, A. and Tahjib-Ul-Arif, M. (2021). Foliar application of auxin or cytokinin can confer salinity stress tolerance in Vicia faba L. Agronomy. 11: 790. 

  2. Angulo-Bejarano, P.I., Puente-Rivera, J. and Cruz-Ortega, R. (2021). Metal and metalloid toxicity in plants: An overview on molecular aspects. Plants. 10: 635.

  3. Ashraf, S., Ali, Q., Zahir, Z.A., Ashraf, S. and Asghar, H.N. (2019). Phytoremediation: Environmentally sustainable way for reclamation of heavy metal polluted soils. Ecotoxicology and Environmental Safety. 174: 714-727. 

  4. Aslam, M., Aslam, A., Sheraz, M., Ali, B., Ulhassan, Z., Najeeb, U., Zhou, W. and Gill, R.A. (2021). Lead toxicity in cereals: Mechanistic insight into toxicity mode of action and management. Front. Plant Science. 11: 587785.

  5. Atteya, A.M. (2002). Characterization of growth and water relations in barley during water stress and after rewatering. Arizona Journal of Pharmacy Science. 29: 285-296.

  6. AVRDC (Asian Vegetable Research and Development Centre), (2012). Mungbean AVDRC Progress Report (2012). Shanhua, Taiwain: AVRDC.

  7. Bhadane, R.S., Prajapati, K.R., Rajput, S.D. and Patil, K. (2021). Influence of seed hardening and foliar spraying of PGRs on morpho-physiological parameters in green gram (Vigna radiata L.). Indian Journal of Agricultural Research. DOI: 10.18805/IJARe.A-5865.

  8. Bielach, A., Hrtyan, M., Tognetti, V.B. (2017). Plants under stress: Involvement of auxin and cytokinin. International Journal of Molecular Science. 18: 1427.

  9. Blatt, M.R. (1993). Hormonal control of ion chanel gating. Annual review of Plant Physiology and Plant Molecular Biology. 44: 543-567.

  10. Blatt, M.R. and Armstrong, F. (1993). K1 channels of stomatal guard cells: Abscisic acid-evoked control of the outward rectifier mediated by cytoplasmic pH. Planta. 191: 330-341.

  11. Bunsangiam, S., Thongpae, N., Limtong, S. et al. (2021). Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth. Scientific Reports. 11: 13094.

  12. Burzyñski, M. and Grabowski, A. (1984). Influence of lead on N03 uptake and reduction in cucumber seedlings. Acta Societatis Botanicorum Poloniae. 53: 77-86.

  13. Carlson, R.W., Bazzaz, F.A. and Rolfe, G.L. (1975). The effect of heavy metals on plants. Part 11. Net photosynthesis and transpiration of whole corn and sunflower plants treated with Ph, Cd, Ni and Ti. Environmental Research. 10: 113-120.

  14. DalCorso, G., Fasani, E., Manara, A., Visioli, G. and Furini, A. (2019). Heavy metal pollutions: state of the art and innovation in phytoremediation. International Journal of Molecular Science. 20: 3412. 

  15. Duncan, D.B. (1955). Multiple range and multiple F-test. Biometrics. 11: 1-42.

  16. Fahad, S., Hussain, S., Matloob, A., Khan, F.A., Khaliq, A., Saud, S., Hassan, S., Shan, D., Khan, F., Ullah, N. et al. (2015). Phytohormones and plant responses to salinity stress: A review. Plant Growth Regulation. 75: 391-404. 

  17. Fodor, F.E., Sarvari, F., Lang, Z. and Szigeti, E.C. (1996). Effects of Pb and Cd on cucumber depending on the Fe-complex in the culture solution. Journal of Plant Physiology. 148: 434-439.

  18. Giannakoula, A., Therios, I. and Chatzissavvidis, C. (2021). Effect of lead and copper on photosynthetic apparatus in citrus (Citrus aurantium L.) plants. The role of antioxidants in oxidative damage as a response to heavy metal stress. Plants. 10: 155.

  19. Godbold, D.L. and Kettner, C. (1991). Lead influences root growth and mineral nutrition of Picea abies seedlings. Journal of Plant Physiology. 139: 95-99.

  20. Hampp, R., Kriebitzsch, C. and Ziegler, H. (1974). Effects of lead on enzymes of porphyrin biosynthesis in chloroplasts and erythrocytes. Naturwissenschaften. 11: 504.

  21. Hasan, M.M., Uddin, M.N., Ara-Sharmeen, F. I, Alharby, H., Alzahrani, Y., Hakeem, K.R., et al. (2019). Assisting phytoremediation of heavy metals using chemical amendments. Plants. 8: 295.

  22. Heckathorn, S.A., Mueller, J.K., LaGuidice, S., Zhu, B., Barrett, T., Blair, B. and Dong, Y. (2004). Chloroplast small heat- shock proteins protect photosynthesis during heavy metal stress. American Journal of Botany. 91: 1312-1318. 

  23. Ilyas, N. et al. (2018). Contribution of nitrogen fixed by mung bean to the following wheat crop. Communication in Soil Science and Plant Analysis. 49:148-158.

  24. Iqbal, N., Umar, S., Khan, N.A. and Khan, M.I.R. (2014). A new perspective of phytohormones in salinity tolerance: Regulation of proline metabolism. Environmental and Experimental Botany. 100: 34–42. 

  25. Jahan, M.A.H.S., Hossain, A., Teixeira da Silva,J. A., El-Sabagh, A., Rashid, M.H. and Barutçular, C. (2019). Effect of Naphthaleneacetic acid on root and plant growth and yield of ten irrigated wheat genotypes. Pakistan Journal of Botany. 51: 451-459. 

  26. Kappus, H. (1985). Lipid Peroxidation: Mechanisms, Analysis, Enzymology and Biological Relevance. In: Oxidative Stress. [Sies H, (ed)], London: Academic Press. pp: 273-310.

  27. Khan, M.A., Gul, B. and Weber, D.J. (2004). Action of plant growth regulators and salinity on seed germination of Ceratoides Lanata. Canadian Journal of Botany. 82: 37-42. 

  28. Kibria, M.G. (2008). Dynamics of cadmium and lead in some soils of Chittagang and their influx in some edible crops. Ph.D thesis University of Chittagang, Bangladesh.

  29. Kibria, M.G., Islam, M. and Osman, K. (2009). Effects of lead on growth and mineral nutrition of Amaranthus gangeticus L. and Amaranthus oleracea L. Soil and Environment. 28: 1-6.

  30. Kumar, A., Prasad, M. and Sytar, O. (2012). Lead toxicity, defense strategies and associated indicative biomarkers in Talinum triangulare grown hydroponically. Chemosphere. 89: 1056-1065.

  31. Kumar, P.B.A.N., Dushenkov, V., Motto, H. and Raskin, I. (1995). Phytoextraction: The use of plants to remove heavy metals from soils. Environmental Science and Technology. 29: 1232-1238.

  32. Lambrides, C.J. and Godwin, I.D. (2006). Mungbean. In: Genome Mapping and Molecular Breeding in Plants. [Chittarajan K. ed)], Vol. 3. Berlin/Heidelberg, Germany: Springer, pp: 69-90.

  33. Moustaka, J., Ouzounidou, G., Bayçu, G. and Moustakas, M. (2016). Aluminum resistance in wheat involves maintenance of leaf Ca2+ and Mg2+ content, decreased lipid peroxidation and Al accumulation and low photosystem II excitation pressure. BioMetals. 29: 611-623. 

  34. Neha, Chandra, R., Pareek, N. and Raverkar, K.P. (2021). Enhancing mungbean (Vigna radiata L.) productivity, soil health and profitability through conjoint use of Rhizobium and PGPR. Legume Research. DOI: 10.18805/LR-4552.  

  35. Orhue, E.R. and Innch, S. (2010). Uptake of lead by Celosia argentia in an ultisol. Research Journal of Agriculture and Biological Sciences. 6: 103-107.

  36. Poschenrieder, C., Gunse, B. and Barcelo, J. (1989). Influence of cadmium on water relations, stomatal resistance and abscisic acid content in expanding bean leaves. Plant Physiology. 90: 1365-1371.

  37. Priya, C.S. and Ratna Babu, D. (2021). Genetic parameters of variation and character association for seed yield and its attributes in mungbean [Vigna radiata (L.) Wilczek]. Legume Research. DOI: 10.18805/LR-4498.

  38. Rehman, M.Z.U., Rizwan, M., Ali, S., Ok, Y.S., Ishaque, W., Saifullah, et al. (2017). Remediation of heavy metal contaminated soils by using Solanum nigrum: A review. Ecotoxicology and Environmental Safety. 143: 236-248. 

  39. Salman, M.A.S., Anuradha, C. Sridhar, V. Ram Babu, E. and Pushpavalli, S.N.CV.L. (2021). Genetic variability for yield and its related traits in green gram [Vigna radiata (L.) Wilczek]. Legume Research. DOI: 10.18805/LR-4484.

  40. Samardakiewicz, S. (2000). Structural and functional effects of lead on plant roots. Ph.D. Dissertation, Adam Mickiewicz University in Poznan (in Polish). 

  41. Saygideger, S., Dogan, M. and Keser, G. (2004). Effect of lead and pH on lead uptake, chlorophyll and nitrogen content of Typha latifolia L. and Ceratophyllum demersum L. International Journal of Agriculture and Biology. 6: 168-172. 

  42. Stoeva, N. and Bineva, T. (2003). Oxidative changes and photosynthesis in oat plants grown in as-contaminated soil. Bulgarian Journal of Plant Physiology. 29: 87-95.

  43. Weijers, D. and Wagner, D. (2016). Transcriptional responses to the auxin hormone. Annu. Rev. Plant Biology. 67(4): 539-574.

  44. Wierzbowska, J. and Bowszys, T. (2008). Effect of growth regulators applied together with different phosphorus fertilization levels on the content and accumulation of potassium, magnesium and calcium in spring wheat. Journal of Elementology. 13: 411-422.

  45. Wozny, A. (1995). Lead in plant cells. Sorus, Poznan (in Polish).

  46. Wu, H. et al. (2021). High-level production of indole-3-acetic acid in the metabolically engineered Escherichia coli. Journal of Agriculture and Food Chemistry. 69: 1916-1924.

  47. Wuana, R.A. and Okieimen, F.E. (2011). Heavy metals in contaminated soils: A review of sources, chemistry, risks and best available strategies for remediation. Isralean Ecology. 2011: 402647. 

  48. Zhang, A., Jennings A., Barlow, P.W. and Forde, B.G. (1999). Dual pathways for regulation of root branching by nitrate. Plant Biology. 96: 6529-6534.
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