Loading...

Indole Acetic Acid (IAA) Mediated Amelioration of Lead (Pb) Stress- Physiological Indices of Mung Bean [Vigna radiata (L.) Wilczek]

DOI: 10.18805/LR-630    | Article Id: LR-630 | Page : 1152-1158
Citation :- Indole Acetic Acid (IAA) Mediated Amelioration of Lead (Pb) Stress- Physiological Indices of Mung Bean [Vigna radiata (L.) Wilczek].Legume Research.2021.(44):1152-1158
Sana Saleem, Ghulam Yasin, Ikram Ul Haq, Adeela Altaf, Khalid Hussain, Khalid Nawaz yasingmn_bzu@yahoo.com
Address : Department of Botany, Bahauddin Zakariya University, Multan, Pakistan.
Submitted Date : 13-05-2021
Accepted Date : 26-06-2021

Abstract

Background: Heavy metals have their adverse effects on growth and physiology of plant. Plant growth regulators help in improving the growth and physiological phenomenon in plants. A pot culture experiment was devised to explore the ameliorative potential of Indole Acetic Acid (IAA) for toxicity of rhizospheric lead (Pb) on two varieties of Mung bean [Vigna radiate (L.) Wickzek]. 
Methods: Seeds of two varieties i.e., M- 8 and MN-92 were grown in earthen pots filled with sandy loam soil and were arranged under complete randomization. Fifteen days after germination, the lead (Pb) was added @ 10mg/kg and 20mg/kg soil as solution Pb NO3. Indole Acetic Acid @100.0mM was foliarly sprayed twice at 15 and 30 days of plants emergence. Physiological parameters i.e., Photosynthetic Rate, Transpiration Rate, Stomatal conductance, Sub Stomatal CO2 Concentration and biomass production in the form of stem, root and leaf dry weights were determined at the age of physiological maturity for three replicates.
Result: By application of IAA, photostnthetic rate reduction was declined from 24.61% to 17.78% under 10mg Pb stress and from 55.54% to 27.35% under 20mg Pb stress. Stomatal conductance reduction was declined from 0.56% to 0.28% under 10mg Pb stress and from 3.37% to 1.68% under 20mg Pb stress. Alleviation of Pb stress by IAA for transpiration rate was non significant. Similarly, the role of IAA for alleviation of Pb stress in term of dry weights of stem,root and leaves were non significant statistically.

Keywords

Biomass Indole acetic acid Lead Mung Photosynthetic rate Stomatal conductance Transpiration rate

References

  1. Andrews, M., Sprent, J.I., Raven, J.A. and Eady, P.E. (1999). Relationship between shoot to root ratio, growth and leaf soluble protein concentration of Pisum sativum, Phaseolus vulgaris and Triticum aestivum under different nutrient deficiencies. Plant Cell and Environment. 22: 949-958.
  2. Arduini, I., Godbold, D. and Onnis, A. (1994). Cadmium and copper change root growth and morphology of Pinus pinea and Pinus piaster seedlings. Physiologia Plantarum. 92: 675- 680.
  3. Ashraf, S., Ali, Q., Zahir, Z.A., Ashraf, S. and Asghar, H.N. (2019). Phytoremediation: environmentally sustainable way for reclamation of heavy metal polluted soils. Ecotoxology and Environmental Safety. 174: 714-727. 
  4. Atteya, A.M. (2002). Characterization of growth and water relations in barley during water stress and after rewatering. Al- Azhar Journal of Pharmaceutical Sciences. 29: 285-296.
  5. Barcelo, J. and Poschenrieder, C.H. (1990). Plant water relations as affected by heavy metal stress. Journal of Plant Nutrition. 13: 1-37. 
  6. Barcelo, J., Vazquez, M.D. and Poschenrieder, C.H. (1988). Cadmium induced structural and ultrastructural changes in the vascular system of bush bean stems. Acta Botanica. 10: 254-261.
  7. Carlson, R.W., Bazzaz, F.A. and Rolfe, G.L. (1975). The effect of heavy metals on plants. Part 11. Net photosynthesis and transpiration of whole corn and sunflower plants treated with Ph, Cd, Ni and Ti. Environmental Research. 10: 113-120.
  8. Downes, B. and Crowell, D. (1998). Cytokinin regulates the expression of a soybean b-expansin gene by a post-transcriptional mechanism. Plant Molecular Biology. 37: 437-444.
  9. Duncan, D.B. (1955). Multiple Range and Multiple F-Test. Biometrics, 11: 1-42.
  10. Fengxiang, X.B., Han, B., Sridhar, M., David, L. and Monts, S.Y. (2003). Phytoavailability and toxicity of trivalent and hexavalent chromium to Brassica juncea. New Phytologist. 4: 489-499.
  11. Fodor, F., Sarvari, E., Lang, F. Szigeti, Z. and Cseh, E. (1996). Effects of Pb and Cd on cucumber depending on the Fe- complex in the culture solution. Journal of Plant Physiology. 148: 434-439.
  12. Gadallah, M.A.A. and El-Enany, A.E. (1999). Role of kinetin in alleviation of copper and zinc toxicity in Lupinus termis plants. Plant Growth Regulator. 29: 151-160.
  13. Hampp, R., Kriebitzsch, C. and Ziegler, H. (1974). Effects of lead on enzymes of porphyrin biosynthesis in chloroplasts and erythrocytes. Naturwissenschaften. 11: 504.
  14. Jahan, M.A.H.S., Hossain, A., Teixeira da Silva, J.A., Sabagh, A.EL, Rashid, M.H. and Barutçular, C. (2019). Effect of Naphthaleneacetic acid on root and plant growth and yield of ten irrigated wheat genotypes. Pakistan Journal of Botany. 51: 451-459. 
  15. Jutta, L.M. (2000). Indole-3-butyric acid in plant growth and development. Plant Growth Regulators. 32: 219-230. 
  16. Kappus, H. (1985). Lipid peroxidation: mechanisms, analysis, enzymology and biological relevance. In: Sies H, ed. Oxidative stress. London: Academic Press. Pp, 273-310.
  17. Ouariti, H. and Ghorbal, M.H. (1997). Responses of bean and tomato plants to cadmium: growth, mineral nutrition and nitrate reduction. Plant Physiology and Biochemistry. 35: 347-354. 
  18. Pakistan Economic Survey. (2012). Agriculture in survey report 2012. Islamabad: Ministry of Finance, 17-32.
  19. Petri, J.L., Leite, B.G. and Couto, M. (2010). Effect of growth regulators on ‘Gala’ Apple fructification. UFPEL - Universidade Federal de Pelotas, C.P. 354, 96010-900. 
  20. Pichtel, J. (2016). Oil and gas production wastewater: soil contamination and pollution prevention. Applied and Environmental Soil Science. 2: 79-89.
  21. Poschenrieder, C., Gunse, B. and Barcelo, J. (1989). Influence of cadmium on water relations, stomatal resistance and abscisic acid content in expanding bean leaves. Plant Physiology. 90: 1365-71.
  22. Savita, G., Singh, P.V. and Maurya, N.J. (2011). Responses of Pisum sativum L. to exogenous Indole Acetic Acid application under manganese toxicity. Bulletin of Environmental Contamination and Toxicology. 86: 605-609. 
  23. Sudadi, S. (2012). Exogenous application of tryptophan and indole acetic acid (IAA) to induce root nodule formation and increase yield of soybean. Agricultural Science Research Journal. 2: 134-139.
  24. Van der Werf, A., Nagel, O.W. (1996). Carbon allocation to shoots and roots in relation to nitrogen supply is mediated by cytokinins and sucrose: opinion. Plant Soil. 185: 21-32.

Global Footprints