Legume Research-An International Journal

Isolation, Identification and Conversion Characteristics of Anti-inflammatory Active Saponin in Black Soybean Sprout treated by High Hydrostatic Pressure (HHP)

DOI: 10.18805/LR-552    | Article Id: LR-552 | Page : 185-191
Citation :- Isolation, Identification and Conversion Characteristics of Anti-inflammatory Active Saponin in Black Soybean Sprout treated by High Hydrostatic Pressure (HHP).Legume Research-An International Journal.2021.(44):185-191
M.Y. Kim, Y.J. Lee, G.Y. Jang, B.Y. Hwang, J. Lee, H.S. Jeong hsjeong@chungbuk.ac.kr
Address : Depatment of Food Science and Biotechnology, Chungbuk National University, Cheongju 28644, South Korea.
Submitted Date : 11-02-2020
Accepted Date : 20-05-2020
First Online:

Abstract

Background: The use of germination and high hydrostatic pressure(HHP) processing can improve soyasaponin contents and physiological characteristics. However, no study has sought to identify the active compounds from soyasaponin extracts of black soybean treated by HHP after germination. Therefore, this study was performed to isolate and identify the anti-inflammatory active soyasaponin in black soybean sprouts treated with high hydrostatic pressure (HHP).  
Methods: Black soybeans were germinated for 4 days and subjected to 150 MPa for 24 h. Crude soyasaponin extracts were separated into using C18 column chromatography and the active compound was purified by semi-preparative HPLC. After isolating the active compounds, the secretion of inflammatory markers (nitric oxide, TNF-α, IL-1β and IL-6) by LPS-stimulated RAW 264.7 macrophages were measured. 
Result: The anti-inflammatory compounds were identified as Soyasaponin Bb and Bc. The anti-inflammatory compounds were identified as soyasaponin Bb and Bc. Soyasaponin Bc enhanced with increasing HHP treatment time in control. However, enzyme inactivation kept soyasaponin Bc content constantly regardless of HHP treatment time. Therefore, activation of enzyme is a major factor for increasing soyasaponin BC level and anti-inflammatory activity of soybean sprout according to HHP treatment.

Keywords

Anti-inflammation Black soybean sprout High hydrostatic pressure treatment Soyasaponin

References

  1. Berhow, M.A., Cantrell, C.L., Duval, S.M., Dobbins, T.A., Maynes, J., Vaughn, S.F. (2002). Analysis and quantitative determination of group B saponins in processed soybean products. Phytochemical Analysis. 13: 343-348.
  2. Cheftel, J.C. (1995). Review: High-pressure, microbial inactivation and food preservation. Food Science and Technology International. 1: 75-90.
  3. Eisenmenger, M.J., Reyes-De-Corcuera, J.I. (2009). High pressure enhancement of enzymes: a review. Enzyme and Microbial Technology. 45: 331-347.
  4. Gu, E.J., Kim, D.W., Jang, G.J., Song, S.H., Lee, J.I., Lee, S.B., Kim, H.J. (2017). Mass-based metabolomic analysis of soybean sprouts during germination. Food Chemistry. 217: 311-319. 
  5. Guajardo-Flores, D., Serna-Saldívar, S.O., Gutiérrez-Uribe, J.A. (2013). Evaluation of the antioxidant and antiproliferative activities of extracted saponins and flavonols from germinated black beans (Phaseolus vulgaris L.). Food Chemistry. 141: 1497-1503.
  6. Gurfinkel, D.M., Rao, A.V. (2003). Soyasaponins: the relationship between chemical structure and colon anticarcinogenic activity. Nutrition and Cancer. 47: 24-33.
  7. Ishiyama, M., Tominaga, H., Shiga, M., Sasamoto, K., Ohkura, Y., Ueno, K. (1996). A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. Biological and Pharma- -ceutical Bulletin. 9: 1518-1520.
  8. Kang, J.H., Sung, M.K., Kawada, T., Yoo, H., Kim, Y.K., Kim, J.S., Yu, R. (2005). Soybean saponins suppress the release of proinflammatory mediators by LPS-stimulated peritoneal macrophages. Cancer Letters. 230(2): 219-227.
  9. Kim, M.Y., Jang, G.Y., Lee, Y.J., Kim, K.M., Kim, D.J., Lee, J., Jeong, H.S. (2019). Application of high hydrostatic pressure for production of bioactive soyasaponin from black soybean [Glycine max (L.)] Sprout. Journal of Food and Nutrition Research. 7(4): 270-278.
  10. Kinjo, J., Yokomizo, K., Hirakawa, T., SHII, Y., NOHARA, T., UYEDA, M. (2000). Anti-herpes virus activity of fabaceous triterpenoidal saponins. Biological and Pharmaceutical Bulletin. 23: 887-889.
  11. Kurosawa, Y., Takahara, H., Shiraiwa, M. (2002). UDP-glucuronic acid: soyasapogenol glucuronosyltransferase involved in saponin biosynthesis in germinating soybean seeds. Planta. 215: 620-629.
  12. Lee, I.A., Park, Y.J., Yeo, H.K., Han, M.J., Kim, D.H. (2010). Soyasaponin I attenuates TNBS-induced colitis in mice by inhibiting NF-êB pathway. Journal of Agricultural and Food Chemistry. 58:10929-10934.
  13. Mozhaev, V.V., Heremans, K., Frank, J., Mansson, P., Balny, C. (1996). High pressure effects on protein structure and function. Proteins. 24: 81-91.
  14. Oh, S.R., Kinjo, J., Shii, Y., Ikeda, T., Nohara, T., Ahn, K.S., Lee, H.K. (2000). Effects of triterpenoids from Pueraria lobata on immunohemolysis: â-D-glucuronic acid plays an active role in anticomplementary activity in vitro. Planta Medica. 66: 506-510.
  15. Srisook, K., Han, S.S., Choi, H.S., Li, M.H., Ueda, H., Kim, C., Cha, Y.N. (2006). CO from enhanced HO activity or from CORM-2 inhibits both O 2" and NO production and downregulates HO-1 expression in LPS-stimulated macrophages. Biochemical Pharmacology. 71: 307-318.
  16. Zha, L.Y., Mao, L.M., Lu, X.C., Deng, H., Ye, J.F., Chu, X.W., Luo, H.J. (2011). Anti-inflammatory effect of soyasaponins through suppressing nitric oxide production in LPS-stimulated RAW 264.7 cells by attenuation of NF-êB-mediated nitric oxide synthase expression. Bioorganic and Medinical Chemistry Letters. 21: 2415-2418.

Global Footprints