Yield and yield attributes
Effect of varieties
A result indicates that the variety GM 4 exhibited superior performance, with a significantly higher number of pods/plant (18.3), seeds/pod (10.01) and 1,000-seed weight (40.82 g) compared to GM 7 (Table 2). These variations in yield-contributing traits can be attributed to the varietal potential of GM 4, which demonstrated higher genetic adaptability under arid conditions. This adaptability, coupled with improved dry matter accumulation during successive growth stages, likely contributed to its enhanced yield attributes. Similar findings have been reported by
Swathi et al., (2021), emphasized on varieties showed highly significant variations in the agronomic traits indicating the varieties responded differently into different environment. These genetically controlled phenomena explain the higher or lower yield attributes observed in different varieties. Similar variations among mungbean varieties have been documented by
Rathika and Ramesh (2023), who emphasized the role of genetic variability.
The two-year mean analysis revealed that the mungbean variety GM 4 recorded significantly higher seed yield (1076 kg/ha), stover yield (2010 kg/ha), biological yield (3086 kg/ha) and harvest index (34.38%) compared to GM 7 (Table 3). The superior performance of GM 4 is attributed to its ability to form more yield attributes, resulting in higher stover and biomass yields. The ability of GM 4 to synthesize and convert photosynthates efficiently contributed to its higher dry matter production during the experimental years. GM 4 demonstrated efficient utilization of agronomic inputs and superior energy allocation from source to sink. The higher seed yield of GM 4 can be primarily ascribed to its greater number of pods/plant and superior test weight in the present study. Conversely, the lower seed yield of GM 7 may be due to inefficient assimilate partitioning between vegetative growth and seed formation stages, leading to reduced dry matter accumulation, stover and biomass yields. These findings align with the studies by
Choudhary et al., (2020). Under the present study, correlation studies also showed (Table 4) the dependence of seed yield on yield attributes such as number of pods/plant (0.97), numbers of seeds/pod (0.96) and test weight (0.97).
Effect of iron fortification
Soil application of 20 kg/ha FeSO
4 superimposed with foliar sprays of FeSO
4 (0.5%) at flower initiation (FI) and pod formation stage (PF) significantly recorded higher yield attributing traits
viz., number of pods/plant (17.9), seeds/pod (9.79) and 1000-seed weight (40.23 g) demonstrating significant superiority over the other treatments in Table 2. These results are consistent with the findings of
Bahadari et al., (2020) and
Manjhi et al., (2020). They observed improvements in yield attributes of mungbean due to the soil application of FeSO
4 and its foliar spray (0.5%) can be attributed to enhanced mineral nutrition. Numerous studies have shown the positive effects of iron fortification on crops, with evidence suggesting that its application promotes growth and yield-forming traits like no of pods/plant, no. of seeds/pod and 1000 seed weight (
Pal, 2018). The increase in yield parameters due to enhances photosynthesis and the production of metabolites, which may contribute to improved flowering, fruiting and seed formation iron
Zafar et al., (2023). The effect of zinc and iron on yield attributes of pigeon pea was assessed and the results showed significant improvement in key yield parameters-number of pods per plant, number of seeds per pod and 100-seed weight-after the application of zinc and iron
Farooq et al., (2025). Under the present study, correlation studies also showed (Table 4) the dependence of seed yield on yield attributes such as number of pods/plant (0.97), numbers of seeds/pod (0.96) and test weight (0.97). Pooled analysis of the data revealed that the soil application of 20 kg FeSO
4/ha, combined with its two foliar applications (0.5%) at the flower initiation and pod formation stages significantly increased seed yield (1025 kg/ha), stover yield (1923 kg/ha), biological yield (2948 kg/ha) and harvest index (34.70) over control in mungbean in table 3. The results show that soil and foliar application of Fe significantly enhanced primary branches, plant height, pods/plant, pod length, seeds/pod, test weight, seed yield. However, iron content in seed was enhanced due to its foliage application. These results are consistent with the findings of
Yadav et al., (2021) and
Zafar et al., (2023). An increase in grain Fe iron concentration might also be attributed to the availability of Fe at the reproductive stage of mungbean due to foliar application (
Sawires, 2001).
Effects of zinc solubilizing bacteria (ZSB) and zinc level
Data reveal that progressive increment in soil application of ZnSO
4 up to 20 kg/ha in combination with seed inoculation (SI) with ZSB and its foliar application (0.5 %) at flower initiation (FI) and pod formation (PF) stages tended to increases seed yield (1052 kg/ha) significantly over ZSB + soil application with 15 kg ZnSO
4/ha and foliar application (0.5%) at FI and PF stages (966 kg/ha) and control (845 kg/ha) which was found at par with treatment integrated as seed inoculation with ZSB (SI) + 25 kg ZnSO
4 with foliar application 0.5% at FI and PF stages (Zn
3). Significant improvements in growth attributes due to ZSB inoculation and varying levels of zinc fertilization with foliar sprays at different stages these findings align closely with those of
Salunke et al., (2022) and
Prajapati et al., (2022). The improvement in yield attributes could be attributed due to the critical role of zinc in crop nutrition long with foliar sprays as it is involved in various enzymatic reactions, metabolic processes and oxidation-reduction reactions
(Zafar et al., 2023). Carbohydrate metabolism is positively influenced by zinc nutrition, as it enhances photosynthesis, the formation and transport of sucrose and starch biosynthesis, all of which ultimately affect the formation of yield attributes. Similar findings were reported by
Ramesh et al., (2014) and
Rana et al., (2012). Significant improvements in growth attributes due to zinc fertilization were applied through foliar spray which were further enhanced by coupling with zinc solubilizing bacteria (ZSB), had a considerable impact on improving yield attributes and ultimately increasing the yield of mungbean. Zinc plays a significant role in the initiation of primordial and the partitioning of food materials from leaves to reproductive parts, which ultimately results in better fruiting
(Zafar et al., 2023), thereby prominently increasing yield attributes. Similar improvements in the number of pods/plant and seeds/pod in mungbean due to zinc application were also observed by
Haider et al., (2018) and
Usman et al., (2014) reported that 1000-seed weight of mungbean was enhanced with zinc application at 20 kg/ha. The higher seed yield and biological yield was observed in mungbean crop with the application of 20 kg Zn/ha.
Interaction effect of Iron levels x zinc levels
Seed yield, stover yield and biological yield
Interaction effect of iron and zinc fortification levels significantly increases in seed yield by the combined application of ZSB + 20 kg ZnSO
4 with foliar spray (0.5%) at flower initiation and pod formation stages and 20 kg FeSO
4 +0.5% superimposed with foliar spray at flower initiation and pod formation stages produced significantly higher seed yield (1092 kg/ha) but it was found at par with variation irrespective to treatment ZSB (SI) + 25 kg ZnSO
4 with foliar spray at flower initiation and pod formation stages (1150 kg/ha) However, further data recorded that seed yield were decreased considerably under the influence of their higher levels of zinc and iron fortification treatment (1006 kg/ha) on pooled basis (Table 5).
Economics
Effect of varieties
Variety GM 4 produced a net return of ₹66,974/ha, accompanied by a higher benefit-cost (B C) ratio of 3.41, compared to GM 7 in Fig 1 and 2. Both varieties showed notable variation in these parameters, primarily due to differences in seed and stover yield. GM 4 consistently outperformed GM 7 in both seed and stover production, resulting in higher net returns and a more favourable benefit cost ratio. These findings are in line with previous studies that highlighted the correlation between yield productivity and economic profitability (
Rathika and Ramesh, 2023), who reported that the better yield attributes and yields produced by Co(Gg) 8 which might be responsible for higher economics of mungbean.
Effect of iron fortification
The data revealed that soil application of 20 kg FeSO
4/ha combined with a 0.5% foliar spray at flower initiation and pod formation stages (Fe
1) in mungbean resulted in the highest net return (₹ 62,051/ha) and the highest B:C ratio (3.20) during the experimentation (Table 2). These findings align with the results of
Manjhi et al., (2020) and
Bahadari et al., (2020). This improvement can be attributed to the substantial increase in yield resulting from the enhanced availability of iron through both soil and foliar fortification, which addressed the hidden deficiency of iron and promoted better nutrition for the crop.
Effects of zinc solubilizing bacteria (ZSB) and zinc level
Among the treatments, seed inoculation with ZSB, supplemented with 20 kg ZnSO
4/ha and a 0.5% foliar spray at the flower initiation and pod development stages (Zn
2), achieved the highest net returns (₹64,202/ha) and a B C ratio of 3.26 on a pooled basis (Fig 1 and 2). This economically optimal treatment outperformed preceding zinc levels while also yielding higher profitability. The results align with findings reported by
Prajapati et al., (2022). The superior net returns under the Zn
2 treatment were attributed to the higher seed and stover yields, in comparison to the ZSB + 15 kg ZnSO
4/ha (Zn
1) and control treatments. However, the Zn
2 treatment was found to be statistically at par with ZSB + 25 kg ZnSO
4/ha (Zn
3). These findings are consistent with those reported by
Gahlot et al., (2020) and
Khan and Prakash (2014), who observed similar trends in mungbean under soil-applied zinc treatments.