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Legume Research

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Research Article

Legume Research, volume 43 issue 6 (december 2020) : 872-877

Contribution to the Study of the Relationship between Gammaproteobacteria and Rhizobia in Legume Species of the Genus Hedysarum

Torche Esma, Rouabhi Ahlem, Torche Boutheina, Slatnia Soumia, Gharzouli Razika, Benhizia Hayet, Benhizia Yacine, Benguedouar Ammar
1Faculté des Sciences de la Nature et de la Vie et des Sciences de la Terre et de l’Univers, Université 8 Mai 1945 Guelma, Guelma, Algeria. 

  • Submitted19-09-2019|

  • Accepted16-01-2020|

  • First Online 15-05-2020|

  • doi 10.18805/LR-527

Cite article:- Esma Torche, Ahlem Rouabhi, Boutheina Torche, Soumia Slatnia, Razika Gharzouli, Hayet Benhizia, Yacine Benhizia, Ammar Benguedouar (2020). Contribution to the Study of the Relationship between Gammaproteobacteria and Rhizobia in Legume Species of the Genus Hedysarum. Legume Research. 43(6): 872-877. doi: 10.18805/LR-527.

ABSTRACT

The nodular contents of the leguminous species of the genus Hedysarum (H. carnosum, H. spinosissimum subsp capitatum and H. pallidum) consist of cultivable bacteria belonging to the class Gammaproteobacteria, while the rhizobia of these leguminous species are not cultivable. Rhizobium sulla is the specific micro-symbiont of the H. coronarium leguminous species. The aim of this study is to see if the Gammaproteobacteria that inhabit the nodules of the three species of the genus Hedysarum (H. carnosum, H. spinosissimum subsp capitatum and H. pallidum) could have acquired the symbiotic criteria from rhizobia and cause nodulation in these host legume species by the transformation process. Transformation is carried out by extracting the plasmid DNA from the R. sulla strain and incorporate it into the bacterial strains of the Gammaproteobacteria class. Nodulation assay is performed to evaluate the ability of transformed strains to cause nodulation in legume species. The results showed that although there has been a transformation, there is a total failure of nodulation in the three legume species. This is explained by the specificity of molecular signals between bacteria and host plants as well as the genetic information carried by the plasmid is not sufficient to achieve a complete symbiotic relationship.

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