Evaluation of Soybean (Glycine max L.) Genotypes on the Basis of Biochemical Contents and Anti-oxidant Enzyme Activities

DOI: 10.18805/LR-4678    | Article Id: LR-4678 | Page : 1419-1429
Citation :- Evaluation of Soybean (Glycine max L.) Genotypes on the Basis of Biochemical Contents and Anti-oxidant Enzyme Activities.Legume Research.2021.(44):1419-1429
Akash Sharma, M.K. Tripathi, Sushma Tiwari, Neha Gupta, Niraj Tripathi, Nishi Mishra drmanojtripathi64@gmail.com
Address : Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior-474 002, Madhya Pradesh, India.
Submitted Date : 29-05-2021
Accepted Date : 24-07-2021


Background: Soybean is an important leguminous crop. Abnormal weather has played an enormous role in the strident decline in crop yields. Drought is considered as a significant abiotic factor responsible for yield reduction in soybean.
Methods: The present work was carried out in order to screen soybean genotypes for their drought tolerance ability by means of different biochemical and antioxidant enzymatic activities responses.
Conclusion: On the basis of biochemical parameters and anti-oxidant enzymatic activities, soybean genotype viz., RVS-211-77, RVS-211-75, NRC-7, SL-96, NRC-136, AMS100-39, SL-96, RVS-2012-01, RVS-211-73 and JS97-52 have been identified with better performance and can be used as parents for further crop improvement programme to breed drought tolerant variety.


​Anti-oxidant Breeding Crop improvement Drought Soybean


  1. Aebi, H.E. (1983). Catalase. In: Method of Enzymatic Analysis. VCH, Weinheim, Germany-Deerfield, FL. 3: 273-286.
  2. Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology. 24: 1-15.
  3. Anjum, S.A., Xie, X., Wang, L., Saleem, M.F., Chen, M. and Wang, L. (2011). Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research. 6(9): 2026-2032.
  4. Bates, L.S., Waldren, R.P. and Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil. 39: 205-207.
  5. Choudhary, M.L., Tripathi, M.K., Tiwari, S., Pandya, R.K., Gupta, N., Tripathi, N. and Parihar, P. (2021). Screening of pearl millet [Pennisetum glaucum (L.) R. Br.] germplam lines for drought tolerance based on morpho-physiological traits and SSR markers. Current Journal of Applied Science and Technology. 40(5): 46-63. https://doi.org/10.9734/cjast/2021/v40i531303.
  6. Kachare, S. (2017). Studies on morpho-physiological changes and gene expression under drought condition in soybean [Glycine max (L.) Merrill]. A Ph. D Thesis, JNKVV, Jabalpur.
  7. Kachare, S., Tiwari, S., Tripathi, N. and Thakur, V.V. (2019). Assessment of genetic diversity of soybean [Glycine max (L.) Merr.] genotypes using qualitative traits and microsatellite makers. Agricultural Research. DOI: 10.1007/s40003-019-00412-y.
  8. Mishra, N., Tripathi, M.K., Tiwari, S., Tripathi, N. and Trivedi, H.K. (2020). Morphological and molecular screening of soybean genotypes against yellow mosaic virus disease. Legume Research. DOI: 10.18805/LR-4240.
  9. Mishra, N., Tripathi, M.K., Tiwari, S., Tripathi, N., Ahuja, A., Sapre, S. and Tiwari, S. (2021a). Cell suspension culture and in vitro screening for drought tolerance in soybean using poly-ethylene glycol. Plants.10 (3): 517-536.
  10. Mishra, N., Tripathi, M.K., Tiwari, S., Tripathi, N., Gupta, N. and Sharma, A. (2021b). Morphological and physiological performance of Indian soybean [Glycine max (L.) Merrill] genotypes in respect to drought. Legume Research. DOI:10.18805/LR-4550.
  11. Mishra, N., Tripathi, M.K., Tripathi, N. Tiwari, S., Gupta, N., Sharma, A. and Shrivastav, M.K. (2021c). Changes in biochemical and antioxidant enzymes activities play significant role in drought tolerance in soybean. International Journal of Agricultural Technology. 17(4): 1425-1446.
  12. Nakano, Y. and Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology. 22: 867-880.
  13. Pinheiro, C., Rodrigues, A.P., de Carvalho, I.S., Chaves, M.M. and Ricardo, C.P. (2005). Sugar metabolism in developing lupin seeds is affected by a short-term water deficit. Journal of Experimental Botany. 56: 2705-2712.
  14. Sahu, V.K., Tiwari, S., Gupta, N., Tripathi, M.K. and Yasin, M. (2020). Evaluation of physiological and biochemical contents in desi and kabuli chickpea. Legume Research. 10. doi.18805/LR-4265.
  15. Smith, I.K., Vierheller, T.V. and Thorne, C.A. (1988). Assay of glutathione reductase in crude tissue homogenates using 5, 5'-dithiobis (2-nitrobenzoic acid). Annals of Biochemistry. 175: 408-413.
  16. Snedecor, G.W. and Cochran, W.G. (1967). Statistical Methods. VI Ed. Oxford IBH Pub. Co. Delhi.
  17. Tiwari, S. and Tripathi, M.K. (2005). Comparison of morphogenic ability of callus types induced from different explants of soybean [Glycine max (L.) Merrill]. Legume Research. 28(2): 115-118.
  18. Tripathi, M.K. and Tiwari, S. (2004). Morphogenesis and plantlet regeneration from soybean [Glycine max (L.) Merrill] leaf discs influenced by genotypes and plant growth regulators. Legume Research. 27(2): 88-93. 
  19. Upadhyay, S., Singh, A.K., Tripathi, M.K., Tiwari S. and Tripathi, N. (2020a). Validation of simple sequence repeats markers for charcoal rot and Rhizoctonia root rot resistance in soybean genotypes. International Journal of Advanced Biotechnology Research. 10(2): 137-144.
  20. Upadhyay, S., Singh, A.K., Tripathi, M.K., Tiwari, S., Tripathi, N. and Patel, R.P. (2020b). In vitro selection for resistance against charcoal rot disease of soybean [Glycine max (L.) Merrill] caused by Macrophomina phaseolina (Tassi) Goid. Legume Research. DOI: 10.18805/LR-4440.
  21. Vasconcelos, A.C.F., Zhang, X.Z., Ervin, E.H. and Kiehl, J.D. (2009). Enzymatic antioxidant responses to biostimulants in maize and soybean subjected to drought. Scientia Agricola. 66(3): 395-402.
  22. Zhang, M., Duan, L., Tian, X., He, Z., Li, J., Wang, B. and Li, Z. (2007). Uniconazole-induced tolerance of soybean to water deficit stress in relation to changes in photosynthesis, hormones and antioxidant system. Journal of Plant Physiology. 164: 709-717.

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