Loading...

Physiological Divergence in Green Gram [Vigna radiata (L.) Wilczek] Genotypes for Drought and High Temperature Stress Tolerance During Flowering Phase

DOI: 10.18805/LR-4314    | Article Id: LR-4314 | Page : 960-967
Citation :- Physiological Divergence in Green Gram [Vigna radiata (L.) Wilczek] Genotypes for Drought and High Temperature Stress Tolerance During Flowering Phase.Legume Research.2022.(45):960-967
M. Jincy, V. Babu Rajendra Prasad, A. Senthil, P. Jeyakumar, N. Manivannan prasadvenugopal@gmail.com
Address : Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India.
Submitted Date : 6-01-2020
Accepted Date : 23-09-2020

Abstract

Background: Drought and high temperature stress limits the crop production. Development of drought and high temperature tolerant cultivars that can withstand and yield better under adverse conditions is very much important to ensure the food and nutritional security. Green gram is one of the important pulse crops with high nutritional and economic value. Among the various stages of plant growth and development, reproductive stage is highly sensitive to drought and high temperature stress across all species. The main objective of this study was to evaluate green gram germplasm collection and identification of elite greengram genotypes that can withstand drought and high temperature stresses at reproductive stage. 
Methods: The experiment was conducted during March-April, 2019, at National Pulses Research Centre, Vamban, Pudukottai district, Tamil Nadu. To study the influence of combined drought and high temperature stress during reproductive stage, the green gram genotypes were sown in pots. Six pots were maintained for each genotype of which three were maintained at 100% field capacity (control) and for another three; drought stress (50% field capacity for 5 days) was imposed combined with high temperature stress (36 ± 2°C) during reproductive phase (35 Days after sowing). At the end of stress period, physiological and biochemical analysis were carried out to identify the tolerant green gram genotypes against drought and high temperature stresses.
Result: In the present study, drought and high temperature stress has negative impact on green gram physiology. Among the genotypes screened for their tolerance at reproductive stage, the following green gram genotypes viz., TARM 1, VGG 15029, VGG 17003, VGG 17004, VGG 17006, VGG 17010 and VGG 17019 were found to withstand drought and high temperature stress and maintain high total chlorophyll content, relative water content and chlorophyll stability index. These green gram gramplasm can be used in pulse breeding program to evolve resilient green gram varieties.  
Screening of 29 green gram genotypes for drought and high temperature stress during reproductive stage were carried out by maintaining the drought stress (50% field capacity for 5 days) combined with high temperature stress (36 ± 2°C) during reproductive stage (35 days after sowing) by pot culture experiment. Total chlorophyll, relative water content, chlorophyll stability index (CSI), oxidants and antioxidant activity were quantified to identify the tolerant green gram genotypes against drought and high temperature stresses. Based on physiological and biochemical parameters, the following green gram genotypes viz., TARM 1, VGG 15029, VGG 17003, VGG 17004, VGG 17006, VGG 17010 and VGG 17019 were found to withstand and tolerate combined drought and high temperature stresses at flowering stage.

Keywords

Chlorophyll content Drought High temperature Relative water content Reproductive stage

References

  1. Aebi and Hugo. (1984). Catalase in vitro. Methods in Enzymology. 121-126. 
  2. Barrs, H.D. and Weatherley, P.E. (1962). A re-examination of the relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Sciences. 15(3): 413-428.
  3. Bates, L.S., Waldren, R.P. and Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil. 39(1): 205-207.
  4. Behera, T.H., Panda, S.K. and Patra, HK. (1999). Chromium ion induced lipid peroxidation in developing wheat seedlings: role of growth hormones. Indian Journal of Plant Physiology. 4(3): 236-238.
  5. Chaitanya, K.K. and Naithani, S.C. (1994). Role of superoxide, lipid peroxidation and superoxide dismutase in membrane perturbation during loss of viability in seeds of Shorea robusta Gaertn.f. New Phytologist. 126(4): 623-627.
  6. Chauhan, Y.S. and Senboku, T. (1996). Thermostabilities of cell-membrane and photosynthesis in cabbage cultivars differing in heat tolerance. Journal of Plant Physiology. 149(6): 729-734.
  7. Choudhury, S., Panda, P., Sahoo, L. and Panda, S.K. (2013). Reactive oxygen species signaling in plants under abiotic stress. Plant Signaling and Behavior. 8(4): 23681.
  8. Deivanai, S., Devi, S.S. and Rengeswari, P.S. (2010). Physiochemical Traits as Potential Indicators for Determining Drought Tolerance during Active Tillering Stage in Rice (Oryza sativa L.). Pertanika Journal of Tropical Agricultural Science. 33(1).
  9. Djanaguiraman, M., Annie Sheeba, J., Durga Devi, D., Bangarusamy, U. and Prasad, P.V.V. (2010). Nitrophenolates spray can alter boll abscission rate in cotton through enhanced peroxidase activity and increased ascorbate and phenolics levels. Journal of Plant Physiology. 167(1): 1-9.
  10. Djanaguiraman, M., Prasad, P.V.V., Boyle, D.L. and Schapaugh, W.T. (2013). Soybean pollen anatomy, viability and pod set under high temperature stress. Journal of Agronomy and Crop Science. 199(3): 171-177.
  11. Fahad, S., Bajwa, A.A., Nazir, U., Anjum, S.A., Farooq, A., Zohaib, A., Sadia, S., Nasim, W., Adkins, S., Saud, S. and Ihsan M.Z. (2017). Crop production under drought and heat stress: Plant responses and management options. Frontiers in Plant Science. 8: 1147.
  12. Farooq, M., Aziz, T., Basra, S.M.A., Cheema, M.A. and Rehman, H. (2008). Chilling tolerance in hybrid maize induced by seed priming with salicylic acid. Journal of Agronomy and Crop Science. 194(2): 161-168.
  13. Farooq, M., Wahid, A., Kobayashi, N., Fujita, D. and Basra, S.M.A. (2009). Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development. 29: 185-212.
  14. Fu, J. and Huang, B. (2001). Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environmental and Experimental Botany. 45(2): 105-114.
  15. Hatfield, J.L., Boote, K.J., Kimball, B.A., Ziska, L.H., Izaurralde, R.C., Ort, D., Thomson, A.M. and Wolfe, D. (2011). Climate impacts on agriculture: implications for crop production. Agronomy Journal. 103(2): 351-370.
  16. Kumar, S., Kaushal, N., Nayyar, H. and Gaur, P. (2012). Abscisic acid induces heat tolerance in chickpea (Cicer arietinum L.) seedlings by facilitated accumulation of osmoprotectants. Acta Physiologiae Plantarum. 34(5): 1651-1658.
  17. Kumari, P. and Varma, S.K. (1983). Genotypic differences in flower production/shedding and yield in mungbean (Vigna radiata). Indian Journal of Plant Physiology. 26: 402-405.
  18. Miller, G., Shulaev, V. and Mittler, R. (2008). Reactive oxygen signaling and abiotic stress. Physiologia plantarum. 133(3): 481-489.
  19. Moller and Ian, M. (2001). Plant mitochondria and oxidative stress: electron transport, NADPH turnover and metabolism of reactive oxygen species. Annual Review of Plant Biology. 52(1): 561-591.
  20. Moller, I.M., Jensen, P.E. and Hansson, A. (2007). Oxidative modifications to cellular components in plants. Annual Review of Plant Biology. 58: 459-481.
  21. Murthy, K.S. and Majumdar, S.K. (1962). Modification of the technique for determination of chlorophyll stability index in relation to studies of drought resistance in rice. Current Science. 31: 470-471.
  22. Nahar, K., Hasanuzzaman, M., Alam, M.M. and Fujita, M. (2015). Glutathione-induced drought stress tolerance in mung bean: coordinated roles of the antioxidant defence and methylglyoxal detoxification systems. AoB Plants. 7.
  23. Nahar, K., Hasanuzzaman, M., Alam, M.M., Rahman, A., Mahmud, J.A., Suzuki, T. and Fujita, M. (2017). Insights into spermine-induced combined high temperature and drought tolerance in mung bean: osmoregulation and roles of antioxidant and glyoxalase system. Protoplasma. 254(1): 445-460.
  24. Patterson, B.D., MacRae, E.A. and Ferguson, I.B. (1984). Estimation of hydrogen peroxide in plant extracts using titanium (IV). Analytical Biochemistry. 139(2): 487-492
  25. Prasad, P.V.V., Pisipati, S.R., Momcilovic, I. and Ristic, Z. (2011). Independent and combined effects of high temperature and drought stress during grain filling on plant yield and chloroplast EF-Tu expression in spring wheat. Journal of Agronomy and Crop Science. 197 (6): 430-441.
  26. Quinn, P.J and Williams, W.P. (1985). Environmentally induced changes in chloroplast membranes and their effects on photosynthetic function. Photosynthetic Mechanisms and The Environment. 6: 1-47.
  27. Reddy, A.R., Chaitanya, K.V. and Vivekanandan, M. (2004). Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology. 161 (11): 1189-1202.
  28. Sayed and Suzan, A. (1999). Effects of lead and kinetin on the growth and some physiological components of safflower. Plant Growth Regulation. 29(3): 167-174.
  29. Shan, Z., Luo, X. and Wei, M. et al (2018). Physiological and proteomic analysis on long-term drought resistance of Casava (Manihot esculenta Craztz). Sci Rep. 8: 17982. (12 p). 
  30. Wahid, A., Gelani, S., Ashraf, M. and Foolad, M.R. (2007). Heat tolerance in plants: an overview. Environmental and Experimental Botany. 61(3): 199-223.
  31. Yang, F. and Ling, F.M. (2010). Adaptive responses to progressive drought stress in two poplar species originating from different altitudes. Silva Fennica. 44(1): 23-37.
  32. Yoshida, S., Forno, D.A. and Cock, J.H. (1971). Laboratory manual for physiological studies of rice. International Rice Research Institute. 2nd Ed.

Global Footprints