Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.32, CiteScore: 0.906

  • Impact Factor 0.8 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus

Influence population behaviour of soybean-rhizobia on nitrogen fraction under different cropping system in Vertisols of central India

Vinod Kumar*, A.K. Rawat, D.L.N. Rao
  • Submitted|

  • First Online |

  • doi

Cite article:- Kumar* Vinod, Rawat A.K., Rao D.L.N. (NaN). Influence population behaviour of soybean-rhizobia on nitrogen fraction under different cropping system in Vertisols of central India . Legume Research. (): . doi: undefined.

To assess the indigenous composition (slow and fast growers) of soybean-rhizobia in Vertisols under different cropping system and their impact on nitrogen (N) fraction in soil. The soils were from soybean-based (soybean-wheat and soybean-chickpea) or cereal-based (maize-wheat, rice-wheat and maize-chickpea) rotations during 2013-15. Sampling site for soybean-wheat system were three selective treatments-absolute control, recommended dose of fertilizers (RDF) and RDF+FYM were considered. Ratio of fast-growing soybean-rhizobia was more in those cropping system where soybean was one of the crops while population of slow-growers were more with cereal-cereal or maize-chickpea cropping system. With regard to nodulation and N symbiotic efficiency; better performance (number of nodules, dried weight, N-content and Ntot-uptake) was found by slow-growing as compared to fast growing rhizobia. Irrespective of cropping system, maximum SOC was found at maximum vegetative growth in soybean-wheat rotation (5.7 g kg-1 soil) with application of RDF+FYM, while minimum (3.2 g kg-1 soil) with in control of soybean-wheat. Available and soil-Ntot was found more in kharif season where soybean was included in the cropping system as compared to other system. Soil aeration and legume-based more supported conversion of NH4+-N to NO3--N during the rabi season as compared to kharif and cereal-based respectively. These results imply that inclusion of any leguminous crop in cropping system improved the N-stock in soil.


  1. Alvey, S. Yang, C.H., Buerkert, A. and Crowley D.E. (2003). Cereal/legume rotation effects on rhizosphere bacterial community structure in West African soils. Biology Fertility Soils, 37: 73-82. 

  2. Ansari, P.G., Rao, D.L.N. and Pal, K.K. (2014). Diversity and phylogeny of soybean rhizobia in central India. Annals Microbiology, 64: 1553-1565.

  3. Aslam, M., Mahmood, I.A., Peoples, B., Schwenke, G.D. and Herridge, D.F. (2003). Contribution of chickpea nitrogen fixation to increased wheat production and soil organic fertility in rain-fed cropping. Biology Fertility Soils, 38: 59-64.

  4. Besnard, E., Chenu C., Balesdent, J., Puget, P. and Arrouays, D. (1996). Fate of particulate organic matter in soil aggregates during cultivation. Euro. J. of Soil Science, 47(4): 495-503.

  5. Burd, G., Dixon, D.G. and Glick, B.R. (2000). Plant growth-promoting bacteria that decrease heavy metal toxicity in plants. Canadian J. Microbiology, 46(3): 237-245.

  6. Cocking, E.C. (2003). Endophytic colonization of plant roots by nitrogen-fixing bacteria. Plant Soil, 252(1): 169-175. 

  7. Di Cello, F., Bevivino, A., Chiarini, L., Fani, R., Paffetti, D. and Tabacchioni, S. (1997). Biodiversity of a Burkholderia cepacia population isolation from maize rhizosphere at different plamt growth stages. Applied and Environ. Microbiology, 63: 4485-4493. 

  8. Dowdle, S.F., (1985). The ecology of Rhizobium japonicum 1 in soybean-rice cropping systems in central China (Doctoral dissertation, University of Hawaii, USA). 

  9. Fosu, M., Kuhne, R.F. and Vlek, P.L. (2007). Mineralization and microbial biomass dynamics during decomposition of four leguminous residues. J. of Biological Sciences, 7(4): 632-637.

  10. Franchini, J.C., Torres, E., Souza, R.A., Crispino, C.C., Souza, L.J. and Hungria, M. (2007). Microbiological parameters as indicators of soil quality under various soil management and crop rotation systems in Southern Brazil. Soil Tillage Research, 92: 18-29. 

  11. Ghosh, P.K., Saha, R., Gupta, J.J., Ramesh, T., Das, A., Lama, T.D., Munda, G.C., Bordoloi, J.S., Verma, M.R. and Ngachan, S.V. (2009). Long-term effect of pastures on soil quality in acid soil of North-East India. Soil Research, 47: 372-379.

  12. Gomez, L.L.A. (1968). Rotation and yields of maize: Effect of a rotation with soybeans or lucerne on maize yield. Bibl. 4 ES. Central, Nac. Invest. Agropec. Palmira, Colombia. Tropical Agriculture, 24(4): 204-220. 

  13. Green, C.J. and Blackmer, A.M. (1995). Residue decomposition effects on nitrogen availability to corn following corn or soybean. Soil Science Society of America Journal, 59(4): 1065-1070.

  14. Hayat, R., Ali, S., Siddique, M.T. and Chatha, T.H. (2008). Biological nitrogen fixation of summer legumes and their residual effects on subsequent rainfed wheat yield. Pakistan Journal Botany, 40(2): 711-722.

  15. Jordan, D.C. and Allen, O.N. (1974). Genus II. Rhizobium. In: Bergey’s Manual of Determinative Bacteriology, 8th ed, (eds), R.E. Buchanan and N.E. Gibbons. The Williams and Wilkins Co., Baltimore 262-264.

  16. Keyser, H.H., Bohlool,, B.B., Hu, T.S. and Weber, D.F. (1982). Fast-growing rhizobia isolated from root nodules of soybean. Science, 215: 1631-1632. 

  17. Keyser, H.H., Sadowsky, M.J. and Bohlool, B.B. (1985). Fast-growing soybean rhizobia. In: Proceedings of World Soybean Research Conference III, (eds), R. Shibles, Westview Press, Boulder.

  18. Loureiro, M.F., Kaschuk, G., Alberton, O. and Hungria, M. (2007). Soybean [Glycine max (L.) Merrill] rhizobial diversity in Brazilian oxisols under various soil, cropping, and inoculation managements. Biology Fertility of Soils, 43: 665-674.

  19. Lupwayi, N.Z., Rice, W.A. and Clayton, G.W. (1998). Soil microbial diversity and community structure under wheat as influenced by tillage and crop rotation. Soil Biology and Biochemistry, 30(13): 1733-1741. 

  20. Manna, M.C., Swarup, A., Wanjari, R.H., Singh, Y.V., Ghosh, P.K., Singh, K.N., Tripathi, A.K. and Saha, M.N. (2006). Soil organic matter in West Bengal Inceptisol after 30 years of multiple cropping and fertilization. Soil Sci. Soci. of Ameri. J., 70(1): 121-129.

  21. Nutman, P.S. and Hearne, R. (1980). Persistence of nodule bacteria in soil under long-term cereal cultivation. In: “Rothamsted Experimental Station, Annual Report 1979, Part 2” 77-90.

  22. Okereke, G.U., Onochie, C., Onunkwo, A. and Onyeagba, E. (2001). Effectiveness of foreign bradyrhizobia strains in enhancing nodulation, dry matter and seed yield of soybean (Glycine max L.) cultivars in Nigeria. Biology and Fertility of Soils, 33(1): 3-9. 

  23. Piper, C.S. (1967). Soil and Plant Analysis, Asia Publishing House, Bombay and Delhi.

  24. Rawat, A.K., Rao, D.L.N. and Sahu, R.K. (2013). Effect of soybean inoculation with Bradyrhizobium and wheat inoculation with Azotobacter on their productivity and N turnover in a Vertisol. Archieves of Agronomy & Soil Science, 59(11): 1559-1571.

  25. Shah, Z., Shah, S.H., Peoples, M.B., Schwenke, G.D. and Hrridge, D.F. (2003). Crop residue and fiertilizer N effects on nitrogen fixation and yields of legume-cereal rotations and soil organic futility. Field Crops Research, 83: 1-11.

  26. Somasegaran, P. and Hoben, H.J. (1985). Methods in legume-rhizo­bium technology Nif TAL project and MIRCEN. University of Hawaii, Maui.

  27. Somasegaran, P. and Hoben, H.J. (1994). Handbook for Rhizobia. In: Methods in Legame-Rhizobium technology. Berlin: Springer-    Verlag, New York.

  28. Srinivasarao, B., Venkateswarlu, Rattan Lal, Singh, A.K., Vittal, K.P.R., Kundu, S., Singh. S.R. and Singh, S.P. (2012). Long-term effects of soil fertility management on carbon sequestration in a rice–lentil cropping system of the Indo-Gangetic plains. Soil Sci. Soci. of Ameri. J., 76: 168-178.

  29. Subbiah, B.V. and Asija, G.L. (1956). A rapid procedure for the estimation of available nitrogen in soils. Current Science, 25: 259-260.

  30. Videira, L.B., Pastorino, G.N., Martinez-Alcantara, V. and Balatti, P.A. (2002). Sinorhizobium fredii isolates can be specially identied by a 260-bp fragment from the nolXWBTUV locus. Applied Microbiological Biotechnology, 59: 265-269.

  31. Vincent, J.M. (1970). A Manual for the Practical Study of Root Nodule Bacteria. IBP handbook No. 15 Blackwell, Scientific Publications, Oxford, UK p: 164.

  32. Vincent, J.M. (1974). Root nodule symbiosis with Rhizobium. In: “The Biology of nitrogen fixation.” [A, Quispel (ed.).] North-    Holland research diomographs frontiers of biology 265-347.

  33. Vitousek, P.M., Aber, J.D., Howarth, R.W. et al. (1997). Human alteration of the global nitrogen cycle: sources and consequences. Ecological Applications, 7: 737-750.

  34. Walkley , A. and Black, I.A. (1934). An examination of the degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science, 37: 29 38 

Editorial Board

View all (0)