Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.391

  • Impact Factor 0.8 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Legume Research, volume 39 issue 4 (august 2016) : 543-549

Effect of different processing methods on proximate, mineral and antinutrient content of lima bean (Phaseolus lunatus) seeds

Baddi Jayalaxmi, D. Vijayalakshmi, Ravindra Usha, M.L. Revanna, R. Chandru1 and P.H. Ramanjini Gowda2
1<p>Department of Food Science and Nutrition,&nbsp;UAS, GKVK, Bengaluru-560065, India&nbsp;</p>
Cite article:- Jayalaxmi Baddi, Vijayalakshmi D., Usha Ravindra, Revanna M.L., Gowda2 Ramanjini P.H. and Chandru1 R. (2015). Effect of different processing methods on proximate, mineral and antinutrient content of lima bean (Phaseolus lunatus) seeds . Legume Research. 39(4): 543-549. doi: 10.18805/lr.v0iOF.7108.

The present study was formulated to analyze the effect of common processing methods on proximate, mineral and antinutrient content of lima bean. The protein content in the present study enhanced from 24.6g in raw to 26.5g/100g on germination. The most abundant mineral in raw bean for 100g was potassium (1295.13mg), followed by calcium (326.5mg), phosphorus (153.4mg), magnesium (123.1mg), sodium (27.0mg), iron (5.43 mg). There was significant (p < 0.01) increase in calcium and phosphorus (10.81 and 14.50%) in germinated beans. Moist heating methods like cooking and autoclaving reduced 100 per cent trypsin inhibitor. Dhal had 100 per cent reduction of trypsin inhibitor and oxalate followed by saponin 0.4mg/100g (98.47%) and phytates 11.8 mg/100g (93.06%). All processing methods were effective in reduction of antinutrients and improving nutritional value of lima bean thereby increasing its utilization in foods and food formulations.


  1. Adegbehingbe, K. T. (2014). Effect of fermentation on nutrient composition and anti-nutrient contents of ground lima bean seeds fermented with Aspergillus fumigatus, Rhizopus stolonifer and Saccharomyces cerevisiae. Int J Adv Res., 2: 1208-1215.

  2. Adeparusi, E. O. (2001). Effect of processing on the nutrients and anti-nutrients of lima bean (Phaseolus lunatus L.) flour. Nahrung., 45:94- 6. 

  3. Ali, M. and Gupta, S., (2012), Carrying capacity of Indian agriculture: pulse crops. Curr. Sci., 102:874-881

  4. Anonymous, 2012 Animal Feed Resources Information System, FAO. 

  5. AOAC, (1995). Official methods of analysis, 18th edition, Association of Official Analytical Chemists, Washington, DC.

  6. Apata, D. F and Ologhobo, A. A. (1997). Trypsin Inhibitor and the other anti-nutritional factors in tropical legume seeds. Tropical Science., 37: 52-59.

  7. Audu, S. S. and Aremu, M. O. (2011). Effect of processing on chemical composition of red kidney bean (Phaseolus vulgaris L.) flour. Pak. J. Nutr., 10: 1069-1075.

  8. Audu, S. S., Aremu, M. O. and Lajide, L. (2013). Effects of processing on physicochemical and antinutritional properties of black turtle bean (Phaseolus vulgaris L.) seeds flour. Orient. J. Chem., 29: 979-989.

  9. Bau, H., Villaume, C., Nicolas, J. and Mejean, L. (1997). Effect of germination on chemical composition, biochemical constitutes and antinutritional factors of soya bean (Glycine max) seeds. J. Sci. Food Agric., 73: 1-9. 

  10. D’souza, M. R. (2013). Effect of traditional processing methods on nutritional quality of field bean. Adv. in Bio Res., 14: 29-33 

  11. Emiola, I. A., Ologhobo, A. D. and Gous, R.M. (2007). Performance and histological responses of internal organs of broiler chickens fed raw, dehulled, and aqueous and dry-heated kidney bean meals. Poultry Sci., 86:1234–1240.

  12. Ezeagu, E. I. and Ibegbu, M. D. (2010). Biochemical composition and nutritional potential of ukpa: a variety of tropical lima beans (Phaseolus lunatus) from Nigeria. Pol. J. Food Nutr. Sci., 60: 231-235.

  13. Giami, S. Y. (2001). Quality attributes of three new improved lines of Nigerian lima beans (Phaseolus lunatus L.Walp.). Plant Food Hum Nutr., 56: 325–333.

  14. Kakade, M. L., Rachis, J. J., McGhee, J. E and Puski, G. (1974). Determination of trypsin inhibitor activity of soya products: a collaborative analysis of an improved procedure. Cereal Chem, 51: 376.

  15. Kathirvel, P. and Kumudha, P. (2011). A comparative study on the chemical composition of wild and cultivated germplasm of phaseolus lunatus L. Int J. App Bio and Pharm Tech., 2:296-305. 

  16. Mada, S. B., Garba, A., Mohammed, A., Muhammad, A., Olagunju, A. and Mohammed, H. A. (2012). Effects of boiling and roasting on antinutrients and proximate composition of local and some selected improved varieties of Arachis hypogaea L (Groundnut). Int. J. Food Nutr and Safety, 1: 45-53.

  17. Mittal, R., Nagi, H. P. S., Sharma, P. and Sharma, S. (2012). Effect of processing on chemical composition and antinutritional factors in chickpea flour. J. Food Sci and Eng., 2: 180-186.

  18. Moses, O., Olawuni, I. and Iwouno, J. O. (2012). the proximate composition and functional properties of full-fat flour, and protein isolate of lima bean (Phaseolus lunatus). Scientific reports, 1: 1-5.

  19. Ndidi, U. S., Ndidi, C. U., Olagunju, A., Muhammad, A., Billy, F. G. and Okpe, O. (2014). Proximate, antinutrients and mineral composition of raw and processed (Boiled and Roasted) Sphenostylis stenocarpa seeds from Southern Kaduna, Northwest Nigeria. ISRN Nutrition., 1-9.

  20. Nonogaki, H., Bassel, G.W. and Bewley, J. W. (2010). Germination-still a mystery. Plant Science, 179:574-581.

  21. Raghuramulu, N., Madhavan Nair, K. and Kalyana Sundaran, S., (2003), A laboratory techniques, NIN, ICMR publications., Hyderabad.

  22. Ranganna, S. (2003). Handbook of analysis of fruit and vegetable products. 2nd ed., Tata Mc-Graw-Hill Pub. Com. Ltd., New Delhi.

  23. Sadasivam, S. and Manickam, A. (1991). Second edition, Biochemical methods. New age International Publishers.

  24. Saulawa, L.A., Yaradua, A.I. and Shuaibu, L. (2014). Effect of different processing methods on proximate, mineral and anti nutritional factors content of baobab (Adansonia digitata) seeds. Pak. J. Nutr., 13: 314-318.

  25. Sundaram, U., Marimuthu, M., Anupama, V. And Gurumoorth, I. P., (2013). Comparative antioxidant quality evaluation of underutilized /less common south Indian legumes. Int J Pharm Bio Sci., 4: 117 – 126. 

Editorial Board

View all (0)