Legume Research

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Legume Research, volume 38 issue 6 (december 2015) : 844-850

Influence of chemical and biological properties of field grown pea and lentil rhizospheres on nodulation status and resident rhizobial population in acidic soils of Assam, India

D.J. Nath*, D. Gogoi, A. Gayan, A. Chelleng
1<p>Department of Soil Science,&nbsp;Assam Agricultural University, Jorhat-785 013, India.</p>
Cite article:- Nath* D.J., Gogoi D., Gayan A., Chelleng A. (NaN). Influence of chemical and biological properties of field grown pea and lentil rhizospheres on nodulation status and resident rhizobial population in acidic soils of Assam, India . Legume Research. 38(6): 844-850. doi: 10.18805/lr.v38i6.6734.

The influence of rhizospheric properties on nodulation status and resident rhizobial population of pea and lentil grown at twenty three sites of Assam were assessed. Besides the plant infectivity of rhizobia isolated from pea and lentil nodules were tested for symbiotic effectiveness. The survey established significant variation of nodule number ((35.0-223.8/plant)), nodule dry weight (69.6 to 271.8 mg/plant) and most probable number count (9 to 14700/g soil) across the twenty three diverse sites. Rhizospheric properties viz pH, organic carbon, available P2O5 and K2O could establish significant correlation with nodule number (r=0.892*, 0.771*, 0.753* and 0.669*) and most probable number(r=0.784*, 0.580*, 0.615* and 0.649*) counts, correspondingly. Microbial biomass carbon showed significant correlation with nodule number(r=0.672*). Dehydrogenase and phosphomonoesterase activities too showed significant correlation with nodule number(r=0.695* and 0.634*) and most probable number (r=0.575* and 0.534*) count, respectively. The symbiotic effectiveness of isolated rhizobia demonstrated significant variation of nodule score (1.33-3.67). Only 6 isolates could attain nodule score of ³3.0, irrespective of resident rhizobial population.

  1. Adam, G. and Duncan, H. (2001). Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soils. Soil Biol. Biochem.33: 943-951.

  2. Antoun, H., Beauchamp, C J., Goussard, N., Chabot, R and Lalande, R. (1998). Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: effect on radishes (Raphanus sativus L.). Plant and Soil 204: 57–67.

  3. Bandick, A.K., and Dick, R.P. (1999). Field management effects on soil enzyme activities. Soil Biol. Biochem.31:1471-1479.

  4. Bhattacharyya, P., Chakrabarti, K and Chakraborty, A. (2005). Microbial biomass and enzyme activities in submerged rice soil amended with municipal solid waste compost and decomposed cow manure. Chemosphere 60: 310–318.

  5. Brockwell, J. (1963). Accuracy of a plant-infection technique for counting populations of Rhizobium trifolii. Applied Microbiol. 11: 377–383.

  6. Casida, L.E., Klein, D.A. and Santoro, R. (1964). Soil dehydrogenase activity. Soil Sci. 98: 371-376.

  7. Chandel, S. R. S. (2004). A Handbook of Agricultural Statistics. Achal Prakashan Mandir, Kanpur. pp A265-A327.

  8. Dick, R.P. (1997). Soil enzyme activities as integrative indicators of soil health. In: Biological Indicators of Soil Health (C. Pankhurst, B. Doube and V. Gupta, Eds), CAB International, Wallingford, UK pp 121-156.

  9. Huang, H. C. and Erickson, R. S. (2007). Effect of seed treatment with Rhizobium leguminosarum on Pythium damping-off, seedling height, root nodulation, root biomass, shoots biomass, and seed yield of pea and lentil. J. Phytopathology.155: 31–37.

  10. Jackson, M. L.(1973). Soil Chemical Analysis. Prentice Hall of India Pvt Ltd., New Delhi 452pp.

  11. Kalembasa,S.L. and Jenkinson,D.S. (1973). A comparative study of titrimetric and gravimetric methods for determination of organic carbon in soil. J. Sci. Food and Agri.24:1085-1090

  12. Kaschuk, G., Hungria, M., Santos, J. C. P. and Berton-Junior, J.F.(2006).Differences in common bean rhizobial populations associated with soil tillage management in southern Brazil. Soil & Till. Res. 87: 205–217.

  13. Laguerre, G., Bardin, M. and Amarger, N. (1993). Isolation from the soil of symbiotic and nonsymbiotic Rhizobium leguminosarum by DNA hybridization. Can. J. Microbiol. 39: 1142–1149.

  14. Mohammadi, K., Sohrabi,Y., Heidari, G., Khalesro, S. and Majidi, M. (2012). Effective factors on biological nitrogen fixation. African J. Agri. Res. 7(12):1782-1788.

  15. Rice, W. A., Penny, D. C. and Nyborg, M.(1997). Effects of soil acidity on rhizobia numbers, nodulation and nitrogen fixation by alfalfa and red clover.Can.J. Soil Sci. 57: 197-203. 

  16. Siddiqui, Z. A., Baghel, G. and Akhtar, M. S.( 2007). Biocontrol of Meloidogyne javanica by Rhizobium and plant growth-    promoting rhizobacteria on lentil. World J. of Microbiol. Biotech. 23: 435–41.

  17. Slattery, J. F., Coventry, D. R. and Slattery, W. J.(2001). Rhizobial ecology as affected by the soil environment. Australian J. Exp. Agri. 41:289-98.

  18. Slattery, J. F., Pearce, D. J. and Slattery, W. J.(2004). Effects of resident rhizobial communities and soil type on the effective nodulation of pulse legumes. Soil Biol. Biochem. 36:1339-46.

  19. Tabatabai, M.A. and Bremner, J.M. (1969). Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol. Biochem.1: 301-307.

  20. Trasar-Cepeda, C., Leiros, M.C., Seoane, S. and Gil-Sotres, F. (2008). Hydrolytic enzyme activities in agricultural and forest soils. Some implications for their use as indicators of soil quality. Soil Biol. Biochem.40: 2146-2155.

  21. Vadivelu, S., Sen, T.K., Bhaskar, B.P., Baruah, U., Sarkar, D., Maji, A.K. and Gajbhiye, K.S. (2004). Soil Series of Assam, 101, Natl Bureau of Soil Survey and Land Use Planning, Nagpur, 229p. 

  22. Vance, E.D., Brookes, P.C. and Jenkinson, D.S. (1987). An extraction method for measuring soil microbial biomass carbon. Soil Biol. Biochem.19: 703-707.

  23. Venkateswarlu, B., Had, K. and Katyal, J. C.(1997). Influence of soil and crop factors on the native rhizobial populations    in soils under dryland farming. App. Soil Ecol. 7: 1-10

  24. Vincent, J. M .(1970). A Manual for the Practical Study of Root- Nodule Bacteria. Blackwell Scientific Publications, Oxford and Edinburgh. 164 pp. 

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