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Current IssueEditorial BoardOnline First ArticlesArchive

volume 34 issue 2 (june 2011) : 123 - 128

ISOLATION AND CHARACTERIZATION OF SOME STRAINS AND MUTANTS OF RHIZOBIUM SP. (VIGNA) FOR POLY-Â-HYDROXYBUTYRATE (PHB) PRODUCTION AND SYMBIOTIC PROPERTIES

S
Sandeep Kumar*
A
A.S. Yadav
1C.C.S. Haryana Agricultural University Hisar - 125 004, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Kumar* Sandeep, Yadav A.S. (2025). ISOLATION AND CHARACTERIZATION OF SOME STRAINS AND MUTANTS OF RHIZOBIUM SP. (VIGNA) FOR POLY-Â-HYDROXYBUTYRATE (PHB) PRODUCTION AND SYMBIOTIC PROPERTIES. Legume Research. 34(2): 123 - 128. doi: .

ABSTRACT

Twenty-one wild type (WT) strains of Rhizobium/Bradyrhizobium sp. (Vigna) were screened on minimal medium supplemented with Nile blue A for PHB production by observing yellow/orange fluorescence under UV. The two WT strains MBR16 and MBR25 were good PHB producers. After their NTG mutagenesis nine mutants producing low, intermediate and high amount of PHB were obtained. Out of all the strains/mutants, the mutant MHt1of MBR16 produced the maximum amount of PHB (1.24 g/l). A highly significant positive correlation was found between the amount of PHB produced by the strains/mutants and shoot dry weight and total shoot nitrogen of mungbean plants inoculated with these strains/mutants.

REFERENCES

  1. Anderson, A.J. and Dawes, E.A. (1990). Microbiol. Rev., 54: 450-472.
  2. Bergersen, F.J. and Turner, G.L. (1992). Proc R Soc LondSer B., 249: 143-148.
  3. Bergersen, F.J. and Turner, G.L. (1990). Proc. R. Soc Lond B., 240: 39-59.
  4. Bergersen, F.J., People, M.B. and Turner, G.L. (1991). Proc R Soc Lond Ser B., 245: 59-64.
  5. Beringer, J.E., Hoggan, S.A. and Johnston, A.W.B. (1978). J. Gen. Microbiol., 104: 201-207.
  6. Bremner, J.M. (1960). J. Agric. Sci., 55: 11-13.
  7. Cain, R.B. (1992). 48th Symposium of the Society for general microbiology at University of Cardiff, pp. 293-338.
  8. Cevallos, M., Encarnacion, S., Leija, A., Mora, Y. and Mora, J. (1996). J. Bacteriol., 178(6): 1646-1654.
  9. Kretovich, W.L., Romanov, V.I., Yushkova, L.A., Shramko, V. and Fedulova, N.G. (1977). Plant Soil., 48: 291-302.
  10. Kumar, U., Sharma, A. and Sikka, V.K. (2009). The South Pacific Journal of Natural Science., 27: 35-37.
  11. Law, J.H. and Sleepecky, R.A. (1960). J. Bacteriol., 82: 33-36.
  12. Lee, S.Y. (1996). Biotechnol. Bioeng., 49: 1-14.
  13. Lemoigne M (1923). CR. Hebd. Seances Acad. Sci., 176: 1761.
  14. Mandon, K., Redellet, N.M., Encarnacion, S., Kaminski, P.A., Leija, A., Cevallos, M.A., Elmerich, C. and Mora, J. (1998). J. Bacteriol., 18: 5070-5076.
  15. Mercan, D., Lomaliza, Y. and Mudliar, S. (2002). Turk. J. Biol., 56: 222-228.
  16. Ostle, A.G. and Holt, J.G. (1982). Appl. Environ. Mcirobiol., 44: 238-241.
  17. Poirier, Y., Nawrath, C. and Somerville, C. (1995). Biotechnol., 13: 142-150.
  18. Rebah, F.B., Prévost, D., Tyagi, R.D. and Belbahri, L. (2009). Appl Biochem Biotechnol., 158: 155–16.
  19. Romanov, V.I., Fedulova, N.G., Tchermenskaya, I.E., Shramko,V.I., Molchanov, M.I. and Kretovich, W.L. (1980). Plant Soil., 56: 379-390.
  20. Schwinghamer, E.A. (1960). J.Appl. Microbiol., 8: 349-352.
  21. Sloger, C. (1969). Plant Physiol., 44: 1666-1668.
  22. Tabandeh, F., and Farahani, E.V. (2003). Iranian Polymer Journal., 12 (1): 37-42.
  23. Thakor, N., Trevedi, U. and Patel, K.C. (2006). Indian J. Biotechnol., 5: 137-147.
  24. Tombolini, R., Povolo, S., Buson, A.B., Squartini, A. and Marco P. N. (1995). Microbiol., 141: 2553-2559.
  25. Vincent, J.M. (1970). Blackwell scientific publications, oxford England.
  26. Willis, L.B. and Walker, G.C. (1998). Can. J. Microbiol., 44(6): 554-564.
  27. Wong, P. P. and Evans, H. J. (1971). Plant Physiol., l47: 750-755.
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    volume 34 issue 2 (june 2011) : 123 - 128

    ISOLATION AND CHARACTERIZATION OF SOME STRAINS AND MUTANTS OF RHIZOBIUM SP. (VIGNA) FOR POLY-Â-HYDROXYBUTYRATE (PHB) PRODUCTION AND SYMBIOTIC PROPERTIES

    S
    Sandeep Kumar*
    A
    A.S. Yadav
    1C.C.S. Haryana Agricultural University Hisar - 125 004, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Kumar* Sandeep, Yadav A.S. (2025). ISOLATION AND CHARACTERIZATION OF SOME STRAINS AND MUTANTS OF RHIZOBIUM SP. (VIGNA) FOR POLY-Â-HYDROXYBUTYRATE (PHB) PRODUCTION AND SYMBIOTIC PROPERTIES. Legume Research. 34(2): 123 - 128. doi: .

    ABSTRACT

    Twenty-one wild type (WT) strains of Rhizobium/Bradyrhizobium sp. (Vigna) were screened on minimal medium supplemented with Nile blue A for PHB production by observing yellow/orange fluorescence under UV. The two WT strains MBR16 and MBR25 were good PHB producers. After their NTG mutagenesis nine mutants producing low, intermediate and high amount of PHB were obtained. Out of all the strains/mutants, the mutant MHt1of MBR16 produced the maximum amount of PHB (1.24 g/l). A highly significant positive correlation was found between the amount of PHB produced by the strains/mutants and shoot dry weight and total shoot nitrogen of mungbean plants inoculated with these strains/mutants.

    REFERENCES

    1. Anderson, A.J. and Dawes, E.A. (1990). Microbiol. Rev., 54: 450-472.
    2. Bergersen, F.J. and Turner, G.L. (1992). Proc R Soc LondSer B., 249: 143-148.
    3. Bergersen, F.J. and Turner, G.L. (1990). Proc. R. Soc Lond B., 240: 39-59.
    4. Bergersen, F.J., People, M.B. and Turner, G.L. (1991). Proc R Soc Lond Ser B., 245: 59-64.
    5. Beringer, J.E., Hoggan, S.A. and Johnston, A.W.B. (1978). J. Gen. Microbiol., 104: 201-207.
    6. Bremner, J.M. (1960). J. Agric. Sci., 55: 11-13.
    7. Cain, R.B. (1992). 48th Symposium of the Society for general microbiology at University of Cardiff, pp. 293-338.
    8. Cevallos, M., Encarnacion, S., Leija, A., Mora, Y. and Mora, J. (1996). J. Bacteriol., 178(6): 1646-1654.
    9. Kretovich, W.L., Romanov, V.I., Yushkova, L.A., Shramko, V. and Fedulova, N.G. (1977). Plant Soil., 48: 291-302.
    10. Kumar, U., Sharma, A. and Sikka, V.K. (2009). The South Pacific Journal of Natural Science., 27: 35-37.
    11. Law, J.H. and Sleepecky, R.A. (1960). J. Bacteriol., 82: 33-36.
    12. Lee, S.Y. (1996). Biotechnol. Bioeng., 49: 1-14.
    13. Lemoigne M (1923). CR. Hebd. Seances Acad. Sci., 176: 1761.
    14. Mandon, K., Redellet, N.M., Encarnacion, S., Kaminski, P.A., Leija, A., Cevallos, M.A., Elmerich, C. and Mora, J. (1998). J. Bacteriol., 18: 5070-5076.
    15. Mercan, D., Lomaliza, Y. and Mudliar, S. (2002). Turk. J. Biol., 56: 222-228.
    16. Ostle, A.G. and Holt, J.G. (1982). Appl. Environ. Mcirobiol., 44: 238-241.
    17. Poirier, Y., Nawrath, C. and Somerville, C. (1995). Biotechnol., 13: 142-150.
    18. Rebah, F.B., Prévost, D., Tyagi, R.D. and Belbahri, L. (2009). Appl Biochem Biotechnol., 158: 155–16.
    19. Romanov, V.I., Fedulova, N.G., Tchermenskaya, I.E., Shramko,V.I., Molchanov, M.I. and Kretovich, W.L. (1980). Plant Soil., 56: 379-390.
    20. Schwinghamer, E.A. (1960). J.Appl. Microbiol., 8: 349-352.
    21. Sloger, C. (1969). Plant Physiol., 44: 1666-1668.
    22. Tabandeh, F., and Farahani, E.V. (2003). Iranian Polymer Journal., 12 (1): 37-42.
    23. Thakor, N., Trevedi, U. and Patel, K.C. (2006). Indian J. Biotechnol., 5: 137-147.
    24. Tombolini, R., Povolo, S., Buson, A.B., Squartini, A. and Marco P. N. (1995). Microbiol., 141: 2553-2559.
    25. Vincent, J.M. (1970). Blackwell scientific publications, oxford England.
    26. Willis, L.B. and Walker, G.C. (1998). Can. J. Microbiol., 44(6): 554-564.
    27. Wong, P. P. and Evans, H. J. (1971). Plant Physiol., l47: 750-755.
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