Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 34 issue 2 (june 2011) : 105 - 110

PRE-GERMINATION AND CATECHOLASE (PPO) ACTIVITY IN PHASEOLUS LUNATUS WHOLE SEED, TESTA AND TESTA STRATA

T
T. Aniszewski*
1Research and Teaching Laboratory of Applied Botany, Biological Interactions and Ecological Engineering, Department of Biology, University of Eastern Finland, Joensuu Campus, P.O. Box 111, 80101 Joensuu, Finland.

  • Submitted|

  • First Online |

  • doi

Cite article:- Aniszewski* T. (2025). PRE-GERMINATION AND CATECHOLASE (PPO) ACTIVITY IN PHASEOLUS LUNATUS WHOLE SEED, TESTA AND TESTA STRATA. Legume Research. 34(2): 105 - 110. doi: .

ABSTRACT

The paper characterizes pre-germination behavior and catecholase (PPO) activity in mature seeds, seed testa and several laboratory separated seed testa strata of Phaseolus lunatus. Imbibition coefficients were 1.56 for whole seeds, 3.14 for whole testa, 2.6 for exotesta, 3.25 for meso and endotesta, 3.9 for mesotesta and 2.54 for endotesta.  Enzymatic activity was measured polarographically. Catecholase activity was clearly deatectable and measured in all samples. The lowest activity was in whole seed with the total testa (11.0 nmol O2  h-1 gdm-1) and highest in the separated total testa  (743.0 nmol O2 h-1 gdm-1). Separation of the testa strata showed differences in catecholase activity in the different strata (397.3  nmol O2 h-1 gdm-1 in exotesta, 155.5 nmol O2 h-1 gdm-1 in meso and endotesta combined,  but 119.7 nmol O2 h-1 gdm-1 in the separated mesotesta and only 35.0 nmol O2 h-1 gdm-1 in the separated endotesta). The novel contribution of the present paper is the analysis of the relative contribution of different testa subfractions to the pool of catecholase activity, indicating that catecholase activity in whole seed is lower than in testa. The variations in catecholase activity were relatively large. The results demonstrate the functional role of catecholase and can be used in plant breeding and plant biotechnologies to promote immunity and germination.

REFERENCES

  1. Aniszewski, T. (2005) Plant biology. American Society of Plant Biologists, Seattle, Washington, pp. 198-199.
  2. Aniszewski, T. (2006) Acta Biol. Cracov. Ser. Bot. , 48 (2) : 59-65.
  3. Aniszewski, T. (2009) Seed Sci. & Technol., 37 : 121-131.
  4. Aniszewski, T. (2010) Legume Res., 33 (3) : 157-163.
  5. Aniszewski, T. et al. (2006) Acta Biol. Cracov. Ser. Bot., 48 (1) : 69 -76.
  6. Aniszewski, T. et al. (2008) Acta Biol. Cracov. Ser. Bot., 50 (1) : 7-18.
  7. Betancur-Ancona, D. et al. (2009) Int. J. Food Sci. Tech., 44 (1) : 128-137.
  8. Bentsik, L. et al. ( 2010) Proc. Nat. Acad. Sci. US, 107 (9) : 4264-4269.
  9. Chrisari, M. et al. (2007) J. Agric. Food Chem., 55 (9) : 3469-3476.
  10. Cousens, R. D. et al. (2010) Ann. Bot., 105 (1) : 101-108.
  11. Han, C. Y. et al. (2010) Scientia Hortic., 124 (1) : 83-87.
  12. Maki, H. and Morohashi, Y. (2006) J. Plant Physiol., 163 : 1-10.
  13. Sahbaz R. et al. (2009) J. Appl. Bot. Food Qual., 82 (2) : 163-169.
  14. Schmitz, G.E. et al. (2008) J. Agric. Food Chem., 56: 272-280.
  15. Takahashi, H. (2010) Int. Rev. Cell Mol. Biol., 281 : 129-159.
  16. Waller, J.C. et al. (2010) Proc. Nat. Acad. Sci. US, 107 (23) : 10412-10417.
Published In
Legume Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 34 issue 2 (june 2011) : 105 - 110

    PRE-GERMINATION AND CATECHOLASE (PPO) ACTIVITY IN PHASEOLUS LUNATUS WHOLE SEED, TESTA AND TESTA STRATA

    T
    T. Aniszewski*
    1Research and Teaching Laboratory of Applied Botany, Biological Interactions and Ecological Engineering, Department of Biology, University of Eastern Finland, Joensuu Campus, P.O. Box 111, 80101 Joensuu, Finland.

    • Submitted|

    • First Online |

    • doi

    Cite article:- Aniszewski* T. (2025). PRE-GERMINATION AND CATECHOLASE (PPO) ACTIVITY IN PHASEOLUS LUNATUS WHOLE SEED, TESTA AND TESTA STRATA. Legume Research. 34(2): 105 - 110. doi: .

    ABSTRACT

    The paper characterizes pre-germination behavior and catecholase (PPO) activity in mature seeds, seed testa and several laboratory separated seed testa strata of Phaseolus lunatus. Imbibition coefficients were 1.56 for whole seeds, 3.14 for whole testa, 2.6 for exotesta, 3.25 for meso and endotesta, 3.9 for mesotesta and 2.54 for endotesta.  Enzymatic activity was measured polarographically. Catecholase activity was clearly deatectable and measured in all samples. The lowest activity was in whole seed with the total testa (11.0 nmol O2  h-1 gdm-1) and highest in the separated total testa  (743.0 nmol O2 h-1 gdm-1). Separation of the testa strata showed differences in catecholase activity in the different strata (397.3  nmol O2 h-1 gdm-1 in exotesta, 155.5 nmol O2 h-1 gdm-1 in meso and endotesta combined,  but 119.7 nmol O2 h-1 gdm-1 in the separated mesotesta and only 35.0 nmol O2 h-1 gdm-1 in the separated endotesta). The novel contribution of the present paper is the analysis of the relative contribution of different testa subfractions to the pool of catecholase activity, indicating that catecholase activity in whole seed is lower than in testa. The variations in catecholase activity were relatively large. The results demonstrate the functional role of catecholase and can be used in plant breeding and plant biotechnologies to promote immunity and germination.

    REFERENCES

    1. Aniszewski, T. (2005) Plant biology. American Society of Plant Biologists, Seattle, Washington, pp. 198-199.
    2. Aniszewski, T. (2006) Acta Biol. Cracov. Ser. Bot. , 48 (2) : 59-65.
    3. Aniszewski, T. (2009) Seed Sci. & Technol., 37 : 121-131.
    4. Aniszewski, T. (2010) Legume Res., 33 (3) : 157-163.
    5. Aniszewski, T. et al. (2006) Acta Biol. Cracov. Ser. Bot., 48 (1) : 69 -76.
    6. Aniszewski, T. et al. (2008) Acta Biol. Cracov. Ser. Bot., 50 (1) : 7-18.
    7. Betancur-Ancona, D. et al. (2009) Int. J. Food Sci. Tech., 44 (1) : 128-137.
    8. Bentsik, L. et al. ( 2010) Proc. Nat. Acad. Sci. US, 107 (9) : 4264-4269.
    9. Chrisari, M. et al. (2007) J. Agric. Food Chem., 55 (9) : 3469-3476.
    10. Cousens, R. D. et al. (2010) Ann. Bot., 105 (1) : 101-108.
    11. Han, C. Y. et al. (2010) Scientia Hortic., 124 (1) : 83-87.
    12. Maki, H. and Morohashi, Y. (2006) J. Plant Physiol., 163 : 1-10.
    13. Sahbaz R. et al. (2009) J. Appl. Bot. Food Qual., 82 (2) : 163-169.
    14. Schmitz, G.E. et al. (2008) J. Agric. Food Chem., 56: 272-280.
    15. Takahashi, H. (2010) Int. Rev. Cell Mol. Biol., 281 : 129-159.
    16. Waller, J.C. et al. (2010) Proc. Nat. Acad. Sci. US, 107 (23) : 10412-10417.
    In this Article
      Contact us
      Follow us
      Published In
      Legume Research

      Editorial Board

      View all (0)