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volume 23 issue 4 (december 2000) :
INHERITANCE AND LINKAGE STUDIES WITH SOME ISOZYME AND MORPHOLOGICAL LOCI IN CHICKPEA (CICER ARIETINUM L.)
1Department of Plant Breeding and Genetics Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur 482 004, India
ABSTRACT
Inheritance and linkage relationships of twelve isozyme and four morphological markers were studied in chickpea (Cicer arietinum L.) utilising interspecific F2 segregation of C. arietinum x C. reticulatum crosses involving four accessions of each species. The isozymes were analysed through starch gel electrophoresis and included one isozyme each of acid phosphatase (ACP), alcohol dehydrogenase (ADH), amylase (AMY), aspartate aminotransferase (AAT), esterase (EST), glucosephosphate isomerase (GPI), malate dehydrogenase (MDH) and malic enzyme (ME) and two each of peroxidase (PRX) and phosphoglucomutase (PGM). Only one isozyme was polymorphic in parental lines of C. arietinum, whereas 11 were polymorphic in parental lines of C. reticulaturn. Each of the 12 isozyme loci was found to be under the control of a single locus with codominant alleles. The morphological traits studied included green cotyledons, pale green foliage, bronze foliage and simple leaf. Each of these traits was found to be controlled by a single recessive gene. A linkage was detected between isozyme loci Acp-l and Adh-2.
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Published In
Legume Research