An experiment was conducted during two consecutive years (1997–98 and 1998–99). Seed treatment with Rhizobium and Rhizobium + PSB significantly increased the mean number of nodules/plant by 3.84 and 3.65 per cent and dry weight of nodule by 3.90 and 5.60 per cent over control, respectively. Though, phosphorus and sulphur had no significant effect on number and dry weight of nodules/plant during both the years. Application of phosphorus and sulphur up to 80 kg P2O5/ha and 100 kg S/ha significantly increased the biological yield, nitrogen and phosphorus uptake of fenugreek during both the years. Seed treatment with Rhizobium and Rhizobium + PSB significantly increased the nitrogen uptake over control and PSB inoculation, similarly, PSB and PSB + Rhizobium inoculation significantly increased the phosphorus uptake over control and Rhizobium inoculation during both the years.