Legume Research

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Legume Research, volume 27 issue 2 (june 2007) :

MORPHOGENESIS AND PLANTLET REGENERATION FROM SOYBEAN (GLYCINE MAX L. MERRILL) LEAF DISCS INFLUENCED BY GENOTYPES AND PLANT GROWTH REGULATORS

Tripathi Manoj, Tiwari Sharad
1 Plant Biotechnology Laboratory, Department of Plant Breeding and Genetics,J.N. Agricultural University, Jabalpur - 482 004, India

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Cite article:- Manoj Tripathi, Sharad Tiwari (2024). MORPHOGENESIS AND PLANTLET REGENERATION FROM SOYBEAN (GLYCINE MAX L. MERRILL) LEAF DISCS INFLUENCED BY GENOTYPES AND PLANT GROWTH REGULATORS. Legume Research. 27(2): . doi: .

ABSTRACT

Leaf discs of eleven genotypes of Glycine max were cultured on three different fortifications of MS medium i.e. MS30D (30.0 mg P 2,4-D), MS10PB (10.0 mg l−1 PCPA+ 0.5 mg P BAP) and MS3PB (3.0 mg P PCPA+ 0.5 mg l−1 BAP). High significant differences in the response of genotypes, culture media and genotype x medium interactions were experimented for callus initiation {Qrmation of morphogenic and shoot forming calli and plantlet regeneration from leaf discs. Results designated combination of an auxin at a higher level and BAP at a lower level most responsive for all culture phases of soybean leaf discs.

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