volume 32 issue 1 (march 2009) : 19-24
MOLECULAR MARKER FOR SCREENING FUSARIUM WILT RESISTANCE IN PIGEONPEA [CAJANUS CAJAN (L.) MILLSPAUGH]
1Regional Agricultural Research Station, ANGRAU,
Tirupathi-517 502, India
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Legume Research
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volume 32 issue 1 (march 2009) : 19-24
MOLECULAR MARKER FOR SCREENING FUSARIUM WILT RESISTANCE IN PIGEONPEA [CAJANUS CAJAN (L.) MILLSPAUGH]
1Regional Agricultural Research Station, ANGRAU,
Tirupathi-517 502, India
ABSTRACT
Fusarium wilt is a serious disease in pigeonpea which causes severe yield loses. Screening of 88
lines along with ICPL 87119 and ICPL 8863 resistant checks for fusarium wilt under field conditions
and artificial inoculation identified 14 lines having 0-20% plant mortality and grouped under 0-5
disease score. PCR reactions using different primers with genomic DNA of these lines resulted in
identification of six resistant sources with specific amplification for resistance to wilt at 920bp with
OPGO8 primer. Considering the wilt reaction and resistance linked RAPD marker, it is possible to
identify the new resistance sources in a short time and they can be utilized in breeding programme
or for direct release.
lines along with ICPL 87119 and ICPL 8863 resistant checks for fusarium wilt under field conditions
and artificial inoculation identified 14 lines having 0-20% plant mortality and grouped under 0-5
disease score. PCR reactions using different primers with genomic DNA of these lines resulted in
identification of six resistant sources with specific amplification for resistance to wilt at 920bp with
OPGO8 primer. Considering the wilt reaction and resistance linked RAPD marker, it is possible to
identify the new resistance sources in a short time and they can be utilized in breeding programme
or for direct release.
KEYWORDS
REFERENCES
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- Murray, M.G. and Thompson, W.F. (1986) Nucleic Acid Res. 8:4321-4325.
- Sambrook, J. et al., (1989). Molecular Cloning:a Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press Newyork.
- Saxena K.B., et al., (2002) Food Products Press. 227-260.
- Kotresh et al. (2006) Euphytica. 149:113-120.
- Tyagi, A.P. (1997) J. South Pacific Agri. 4:92-98
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In this Article
APC
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Filling the gap between research and communication ARCC provide Open Access of all journals which empower research community in all the ways which is accessible to all.
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Published In
Legume Research