The effect of high temperature treatment on seeds/seedlings of pea and soybean was investigated by analyzing qualitative protein profiles of heat-stressed and control seed/ seedlings samples by performing Sodium Oodecyl Sulphate Polyacrylamide Gel Electrophoresis (SOS-PAGE). A comparative study of SDS-PAGE protein profiles of both the legume grains showed different response towards heat-stress. The high temperaturetreatment up to 70°C for 10 continuous days induced some changes on the metabolism of the seed tissues producing some new bands of proteins-probably heat shock proteins in pea where as in soybean the same stress promoted the disintegration of proteins and as a result, certain characteristic bands of proteins in the gel of control samples were disappeared from the high temperature-treated seed samples. SOS-PAGE derived seed protein profile is species specific and this analysis is very helpful to elucidate the response of seeds towards heatstress and the production of heat shock proteins by seed tissues. The response towards the heat-stress varied in pea and soybean because pea seeds are protein and starch rich but soybean seeds are protein and lipid rich type.