Chief EditorK.M.L. Pathak
Print ISSN 0367-6722
Online ISSN 0976-0555
NAAS Rating 6.43
Impact Factor 0.5 (2023)
Monitoring Plasma Metabolic Profile, Fertility in Normal and Estrus Synchronized Suckled Kankrej Cows
First Online 26-01-2021|
Methods: The study involved 36 parturient Kankrej cows of the University Farm that were maintained under identical nutritional and managerial practices. Blood samples were collected in heparinized vacutainers from all the animals on the day of calving and then at 10 days interval till 90 days for plasma metabolic profile. Animals monitored by per rectal palpation 10 days apart around day 70-80 postpartum revealed 30 cows to be anestrous or subestrous, which were randomly allocated to five different estrus synchronization protocols at day 90-92, each with six cows and a group of six cows that exhibited estrus and were bred by day 70-90 served as normal control. The treatment protocols included Ovsynch, CIDR, Ovsynch + CIDR, Cosynch and double PGF2α together with estimation of plasma metabolites on day 0, 7, 9/10 (AI) and then at 10 days interval up to day 40 post-AI.
Result: The mean plasma total protein levels varied non-significantly, while total cholesterol and triglycerides varied significantly (p<0.01) between intervals until 90 days postpartum in most of the groups with gradual increase in the mean values from the day of calving. The estrus synchronization protocols, however. did not alter the plasma profile of these constituents. The estrus induction response with Ovsynch, CIDR, Ovsynch + CIDR, Cosynch and PGF2α treatment was 66.66, 83.33, 50.00, 66.66 and 66.66% and the conception rates at induced estrus 16.66, 33.33, 16.66, 50.00 and 50.00%, respectively. In normal cyclic control group, the conception rate at first cycle was 33.33%. The plasma total cholesterol and triglycerides were higher in conceived than non-conceived cows, particularly during early postpartum period and post-AI. It was concluded that CIDR, Cosynch and PGF2α protocols were better for estrus induction and conception rate in anestrous and subestrous suckled Kankrej cows, although they did not influence the plasma metabolic profile.
- Ahlawat, A. and Derashri, H.J. (2009). Metabolic profile in cyclic and non-cyclic vis-a-vis conceiving and non-conceiving cows. The Indian Journal of Field Veterinarians. 4(4): 13-16.
- Ahmad, I., Lodhi, L.A., Qureshi, Z.I. and Younis, M. (2004). Studies on blood glucose, total proteins, urea and cholesterol levels in cyclic, non-cyclic and endometritic crossbred cows. Pakistan Veterinary Journal. 24(2): 92-94.
- Ammu Ramakrishnan, Dhami, A.J., Ankita Killedar and Pande, A.M. (2012b). Postpartum plasma progesterone and metabolic profile in pregnant and non-pregnant Gir cows following estrus synchronization. Indian Journal of Animal Production and Management. 24(1-2): 40-44.
- Ammu Ramakrishnan, Dhami, A.J., Naikoo, M., Patel, S.B. and Savaliya, F.P. (2012a). Effect of puerperium, estrus synchronization and post-breeding periods on plasma profile of total protein, cholesterol and triglycerides in Gir cows. The Indian Journal of Field Veterinarians 7(4): 11-17.
- Bhoraniya, H.L., Dhami, A.J. and Killedar, A. (2012a). Influence of estrus synchronization protocols on fertility, plasma progesterone and biochemical constituents in Kankrej cows. Indian Journal of Animal Reproduction. 33(2): 14-18.
- Bhoraniya, H.L., Dhami, A.J., Naikoo, M., Parmar, B.C. and Sarvaiya, N.P (2012b). Effect of estrus synchronization protocols on plasma progesterone profile and fertility in postpartum anoestrus Kankrej cows. Tropical Animal Health and Production. 44(6): 1191-1197.
- Borakhatariya, D.N., Panchal, M.T. Dhami, A.J., Hadiya, K.K. and Kalasariya, R.M. (2017). Efficacy of estrus synchronization protocols during summer and winter seasons together with biochemical and minerals profile in anoestrus crossbred cows. The Indian Journal of Veterinary Science and Biotechnology. 13(1): 9-16.
- Chaudhary, N.J., Patel, D.M., Dhami, A.J., Vala, K.B., Hadiya, K.K. and Patel, J.A. (2018). Effect of doublesynch and estradoublesynch protocols on estrus induction, conception rate, plasma progesterone, protein and cholesterol profile in anestrus Gir heifers. Veterinary World. 11(4): 542-548.
- Dhami A.J., Lakum, P.D., Patel, P.M., Panchal, M.T. and Kavani, F.S. (2005). Blood biochemical profile in relation to age and reproductive status of HF cattle reared under tropical climate Indian Journal of Animal Reproduction. 26(1): 34-38.
- Dhami, A.J., Hadiya, K.K., Patel, J.A. and Chaudhari, D.V. (2019). Impact of ovulation synchronization protocols in addressing infertility of crossbred dairy cattle. Haryana Veterinarian. 58(1): 56-61.
- Dhami, A.J., Nakrani, B.B., Hadiya, K.K., Patel, J.A. and Shah, R.G. (2015). Comparative efficacy of different estrus synchronization protocols on estrus induction response, fertility and plasma progesterone and biochemical profile in crossbred anoestrus cows. Veterinary World. 8(11): 1310-1316.
- Dhami, A.J., Patel, P.M., Savalia, F.P. and Kavani, F.S. (2007). Biweekly plasma protein profile in anoestrus and suboestrus HF cows without and with GnRH and PGF2 alpha therapy on day 49 postpartum. International Journal of Cow Science. 3(1and2): 44-47.
- Dhami, A.J., Theodore, V.K., Panchal, M.T., Hadiya, K.K., Lunagariya, P.M. and Sarvaiya, N.P. (2017). Effect of peripartum nutritional supplementation on postpartum fertility and blood biochemical and steroid hormone profile in crossbred cows. Indian Journal of Animal Research. 51(5): 821-826.
- Dhoble, R.L., Mane, S.D., Sawale, A.G. and Ingawale, M.V. (2004). Variation in blood biochemical constituents during postpartum period in crossbred cows. Indian Journal of Animal Reproduction. 25(1): 14-16.
- Guedon, L., Saumande, J., Dupron, F., Couquel, C. and Desbals, B. (1999). Serum cholesterol and triglycerides in postpartum beef cows and their relationship to the resumption of ovulation. Theriogenology. 51(7): 1405-1415.
- Hadiya, K.K., Dhami, A.J., Nakrani, B.B. and Lunagariya, P.M. (2015). Estrus induction, follicular dynamics and fertility response to mid-cycle PGF2α, CIDR and Ovsynch protocols in subfertile Gir and crossbred cows. Indian Journal of Animal Reproduction. 36(1): 23-27.
- Kalasariya, R.M., Dhami, A.J., Hadiya, K.K., Borkhatariya, D.N. and Patel, J.A. (2017). Effect of peripartum nutritional management on plasma profile of steroid hormones, metabolites and postpartum fertility in buffaloes. Veterinary World. 10(3): 302-310.
- Kavani, F.S., Khasatiya, C.T., Sthanki, D.J., Thakor, D.B., Dhami, A.J. and Panchal, M.T. (2005). Studies on postpartum biochemical and hormonal profile of fertile and infertile estrous cycles in Surti buffaloes. Indian Journal of Animal Reproduction. 26(1): 1-6.
- Keskin, A., Yilmazbas-Mecitoglu, G., Gumen, A., Karakaya, E., Celik, Y., Okut, H. and Wiltbank, M.C. (2011). Comparison of responses to Ovsynch between Holstein-Friesian and Swedish Red cows. Journal of Dairy Science. 94(4): 1784-1789.
- Khade, N.B., Patel, D.M., Naikoo, M., Dhami, A.J., Sarvaiya, N.P. and Gohel, M.M. (2011). Oestrus induction in pubertal anoestrus Gir heifers using different hormone protocols. The Indian Journal of Field Veterinarians. 7(1): 4-8.
- Khasatiya, C.T., Dhami, A.J., Kavani, F.S. (2005). Reproductive traits and metabolic profile in postpartum fertile and infertile Surti buffaloes. Indian Veterinary Journal. 82(6): 637-641.
- Khasatiya, C.T., Dhami, A.J., Kavani, F.S., Derashri, H.J. and Panchal, M.T. (2007). Weekly monitoring of postpartum plasma profile of glucose and total protein in Surti buffaloes without and with GnRH and PGF2 alpha treatment at day 42. The Indian Journal of Field Veterinarians. 2(3): 1-10.
- Kumar, R., Butani, M.G., Dhami, A.J., Kavani, F.S. and Shah, R.G. (2009). Effect of different therapies on fertility and serum progesterone, metabolites and minerals profile in repeat breeding crossbred cows. The Indian Journal of Field Veterinarians. 5(2): 1-8.
- Kumar, S., Saxena, A. and Ramsagar, (2010). Comparative studies on metabolic profile of anestrous and normal cyclic Murrah buffaloes. Buffalo Bulletin. 29(1): 7-11.
- Mohankrishna., Mishra, U.K., Mishra, O.P., Khan, J.R. and Prakash, B.S. (2010). Efficacy of Ovsynch protocol with fixed time insemination in anestrous Sahiwal cows and heifers. Indian Veterinary Journal. 87: 297-298.
- Naikoo, M., Dhami, A.J. and Ammu Ramakrishnan (2016). Effect of estrus synchronization on plasma progesterone profile and fertility response in postpartum suckled anestrous Kankrej cows. Indian Journal of Animal Research. 50(4): 460-465
- Onita, P. and Colibar, O. (2009). Energy, protein and mineral profile in peripartal period at dairy cows. Lucrări ştiinłifice medicină veterinară. 42(2): 398-404.
- Orkun, D., Mustafa, N., Murat, A., Tayfur, B. and Ahmet, Z. (2006). The effectiveness of Cosynch protocol in dairy heifers and multiparous cows. Turkey Journal of Veterinary and Animal Sciences. 30: 213-217.
- Patel, J.A., Dhami, A.J., Kavani. F.S. and Jani, R.G. (2005). Modulation of conception rate and plasma biochemical profile in repeat breeding Holstein Friesian cows by hormonal therapy. International Journal of Cow Science. 1(2): 47-52.
- Patel, K.R., Dhami, A.J., Hadiya, K.K., Savalia, K.K., Killedar, A. and Patel, S.B. (2014). Effect of mid-cycle PGF2α and GnRH at AI on conception rates, plasma progesterone and biochemical profile in repeat breeding crossbred cows. The Indian Journal of Field Veterinarians. 9(3): 5-11.
- Patel, P.M. and Dhami, A.J. (2005). Postpartum plasma profile of total cholesterol and triglyceride in Holstein Friesian cows with and without hormone therapy under tropical climate. Indian Journal of Animal Reproduction. 26(2): 138-144.
- Patel, P.M. and Dhami, A.J. (2006). Postpartum plasma profile of glucose and total protein in Holstein Friesian cows with and without hormone therapy under tropical climate. Indian Journal of Animal Sciences. 76(2): 118-123.
- Prajapati, A.R., Dhami, A.J., Hadiya, K.K. and Patel, J.A. (2018). Influence of estrus synchronization protocols on plasma profile of progesterone, protein and cholesterol in acyclic HF crossbred cows. The Indian Journal of Veterinary Science and Biotechnology. 13(4): 5-11.
- Pursley, J.R., Mee, M.O. and Wiltbank, M.C. (1995). Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology. 44(7): 915-923.
- Sakaguchi, M. (2011). Practical aspects of the fertility of dairy cattle. Journal of Reproduction and Development. 57(1): 17-33.
- Shamsuddin, M., Bhuiyan, M.M.U., Chanda, P.K., Alam, M.G.S. and Galloway, D. (2006). Radioimmunoassay of milk progesterone as a tool for fertility control in smallholder dairy farms. Tropical Animal Health and Production. 38: 85-92.
- Sivaraman, T., Shanmugasundaram, S., Arunachalam, S. and Sivakumar, T. (2002). Blood profile of Jersey crossbred cows under different physiological stages. Indian Journal of Animal Research. 36(2): 114-117.
- Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods. 8th edn., The Iowa State University Press, Ames, Iowa, USA.
- Srivastava, S.K. and Sahni, K.L. (2000). Blood mineral level affecting pregnancy rates in cows and buffaloes. Indian Journal of Animal Sciences. 70(1): 33-34.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.