Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 52 issue 2 (february 2018) : 276-280

Isolation and characterization of mastitis pathogens and milk composition changes in Murrah buffaloes (Bubalus bubalis) during winter season

Sunita Thakur, Mahendra Singh, Gajendra Kumar Aseri, Archana Verma, Sikander S. Khan
1<p>ICAR-National Dairy Research Institute,&nbsp;Karnal-132 001, Haryana, India.</p>
Cite article:- Thakur Sunita, Singh Mahendra, Aseri Kumar Gajendra, Verma Archana, Khan S. Sikander (2016). Isolation and characterization of mastitis pathogens and milk composition changes in Murrah buffaloes (Bubalus bubalis) during winter season . Indian Journal of Animal Research. 52(2): 276-280. doi: 10.18805/ijar.11320.

ABSTRACT

Present investigation was carried out to study the prevalence of bacterial pathogens in clinical and subclinical mastitis in buffaloes during winter season. A total of 118 Murrah buffaloes were screened using California mastitis Test (CMT). Milk samples were collected and analyzed for fat, protein, lactose, SNF, pH, Electrical conductivity (EC) and Somatic Cell Counts (SCC). Out of 118 milk samples, 60 samples (50.84%) were negative for CMT and 58 samples (49.15%) were detected positive for mastitis incidence. Milk pH and EC was significantly higher (p<0.05) in mastitis affected samples than the normal ones, however, protein, fat, SNF and lactose were lower (p<0.05). SCC ranged between 1.28-1.48 x 105 cells/ml (in normal milk samples as compared to 3.85-6.21x 105 cells/ ml (p<0.05) in mastitis milk samples. Out of 58 samples only 51 samples exhibited bacterial growth. The culturally examined and characterized samples revealed S. aureus (35.29%) as the predominant bacteria followed by S. agalactiae with an isolation rate of 25.49%. The incidence of coliforms bacteria was not detected in any of the sample cultured and examined. It was concluded that mastitis incidence adversely affect quality of milk by increasing the SCC, pH and EC of milk. Appropriate measures needs to be taken to prevent the incidence of S. aureus bacteria which was the major causative agent.

REFERENCES


  1. Ahmed T., Bilal, M., Uallah, S., Rahman, Z. and Muhammad, G.(2007). Impact of mastitis severity on mineral contents of buffalo milk. Pak J Agri. Sci., 44: 176-178.

  2. Ali, Z., Dimri U., and Jhambh,R. (2015). Prevalence and antibiogram of bacterial pathogens from subclinical mastitis in buffaloes. Buffalo Bulletin. 34:1.

  3. Anonymous, (2012). ICAR-National Dairy Research Institute. News Letter 17:(1) 

  4. Chaneton, L., Tirante, L., Maito,J., Chaves, J. and Bussmann, L.E.(2008). Relationship between milk lactoferrin and etiological agent in the mastitic bovine mammary gland. J. of Dairy Science, 91: 1865-1873.

  5. Cheng, D., ShanYuan, Z., ZhaoHua, Y., WenWei, D., ZhiXia, M., ZhiRuI, S.and HuaiChang, S.(2010). Prevalence of bacterial infection responsible for bovine Mastitis. African J. Micro. Res., 4:1110-1116. 

  6. Dhakal, I. P. (2006). Normal somatic cell count and subclinical mastitis in Murrah buffalo. J. Vet. Med., 53: 81-86.

  7. Dhanda, M.R. and Sethi, M.S. (1962). Investigation of mastitis in India. ICAR Res. Series No. 35. New Delhi, India.

  8. Dhillon, A., Gupta, J.K., and Khanna, S.(2000). Enhanced production, purification and characterization of a novel cellulase poor thermostable, alkali-tolerant xylanase from Bacillus circulans AB 16. Process Biochem., 35: 849-856.

  9. Dohoo, I.R. and Meek, A.H. (1982). Somatic cell count in bovine milk. Can. Vet. J., 23:119.

  10. Dua, K. (2001). Incidence, aetiology and estimated economic losses due to mastitis in Punjab and in India-An update. Indian Dairyman., 53:41-48.

  11. Duguma, A., Tolosa, T. and Yohannes, A.(2014). Prevalence of clinical and sub-clinical mastitis on cross bred dairy cows at Holleta Agricultural Research Center, Central Ethiopia. J. of Vet. Med. and Animal Health, 6:13-17.

  12. Ercission, U.H., Lindberg, A., Perrson, W.K., Ekman, T., Arturson, K., Nilsson-ost, M. and Bengtsson, B.(2009). Microbial aetiology of acute clinical mastitis and agent – specific risk factors. Vet. Micro., 137:90-97.

  13. Galvan, M., Jose E., Corona, A., Arana, A.L., Posadas, V.M., Daniel, D., Aguayo, C., Pelaez, C., Erika, A., Martínez, O. and Abner J.G.C.(2015). Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico. BioMed Research, 2015:1-9.

  14. Hussain, R., Javed, M.T. and Khan, A. (2012). Changes in some bio -chemical parameters and somatic cell counts in the milk of buffalo and cattle suffering from mastitis. Pakistan Vet. J., 32: 418–421.

  15. Jingar, S.C., Mehla , R.K., Singh, M. and Singh ,P.K. (2014). Effect of stages and level of milk production on mastitis incidence in cows and murrah buffaloes. J.Bio.Innov 3:117-123.

  16. Keefe, G.P. (1997). Streptococcus agalactiae mastitis: A review. Can. Vet. J., 38: 429-437.

  17. Khan, A.Z. and Muhammad G.(2005). Quarterwise comparative prevalence of mastitis in buffaloes and crossbred cows. Pak. Vet. J., 25: 9-12.

  18. Kuang, Y., Tani, K., Synnott, A.J., Ohshima, K., Higuchi, H., Nagahata, H. and Tanji, Y. (2009). Characterization of bacterial population of raw milk from bovine mastitis by culture-independt PCR-DGGE method. Biochemistry Eng. J., 45: 76-81.

  19. Lindmark-Mansson, H., Branning, C., Alden, G. and Paulsson, M.(2006). Relationship between somatic cell count, individual leukocyte populations and milk components in bovine udder quarter milk. Int Dairy J., 16: 717-727.

  20. Magotra,A., Gupta, I.D., Verma, A., Alex, R., Kumar, V., Vineeth, M.R., Arya,A. and Sakthivel Selvan, A(2015). Characterization and validation of point mutation in breast cancer 1 (BRCA1) and its relationship with mastitis traits in Sahiwal. Int. J. of Recent Res. in Interdisciplinary Sci., 3:22-26.

  21. Memon, M.I., Mirbahar, K.B., Memon, M.R., Akhtar, N., Soomoro, S.A. and Dewani, P. (1999). A study on the etiology of sub-clinical mastitis in buffaloes. Pak. J. Agric. Engg. Vet. Sci., 15: 34-36.

  22. Oliver, S.P., Jayarao, B.M. and Almeida, R.A. (2005).Food borne pathogens in milk and the dairy farm environment food safety and public health implications. Foodborne Path. Dis., 2: 115-129.

  23. Osumi,T., Kishimoto,Y., Kano, R., Maryyama, H., Onozaki, M., Makimura, K., Ito, T., Matsubara, K. and Hasegawa, A.(2008). Prototheca zopfii genotypes isolated from cow barns and bovine mastitis in Japan. Vet. Micro., 131: 419-423.

  24. Owen, J.B., Axford, R.F.E. and Bishop, S.C.(2000). Mastitis in dairy cattle. CAB International Wallingford, 74:243-252.

  25. Perez, J.O., Ahmed, E.K., Hernández, S.R., Mohamed, M., Yasseen, M., Zeidan, A., Salem, M., Bastida, A.Z., Reynoso, D.V., Salazar, M.S., & Luis Camacho-Díaz, L.M., Alonso-Fresán, M.U., DiLorenzo, N.(2015). Prevalence of bovine subclinical mastitis, its etiology and diagnosis of antibiotic resistance of dairy farms in four municipalities of a tropical region of Mexico. Trop Anim. Health. Prod. In press

  26. Quinn, P.J., Carter M.E., Markey B.K.and Carter G.R.(1994). Clinical Vet. Microbiology. Mosby- Yearbook Europe Ltd., Wolf, Spain. 648p.

  27. Radostits, O.M., Gay, C.C., Blood, D.C. and Hinchkliff, K.W. (2000). A Text Book of Veterinary Medicine. 9th Edn.,W.B. Saunders, New York, 563-618.

  28. Radostits, O.M., Gay C.C., Hinchliff, K.W. and Constable, P.D.(2007). Disease of the Mammary gland. Veterinary Medicine. Text Book of the Diseases of Cattle, Horse, Sheep, Pigs and Goats. 10th Ed., Saunders Elsevier, UK, London , 673-762. 

  29. Rawdat, A.M., and Omaima, N.M. (2007). Some biochemical changes in serum and milk of mastitic buffaloes. Egyptian J. Agri. Res., 78: 1737-1747.

  30. Sadashiva, S.O. and Kaliwal , B.B.(2013). Prevalance of bovine mastitis in North Karanatka India. Int. J. Pharm. & H. Care Res., 1: 169-177. 

  31. Sentitula., Yadav, B.R.and R.K.(2012). Incidence of Staphylococci and Streptococci during winter in mastitic milk of Sahiwal cow and Murrah buffaloes. Indian J Microbiol., 52:153–159

  32. Shailza and Singh, M. (2002). Post milking teat dip effect on somatic cell count, milk production and milk composition in cows and buffaloes. Asian.Aus. J.Anim. Sci., 15: 1517-1522.

  33. Singh, P.J. and Singh, P.B. (1994). A study of economic losses due to mastitis in India. Indian J. Dairy Sci., 47 :265-272.

  34. Sutra, L, and Poutrel, B. (1990). Detection of capsular polysaccharaide in milk of cows with intramammary infections caused by Staphylococcus aureus. American J. of Vety. Res., 51: 1857-1859.

  35. Tripaldi, C., Palocci, G., Miarelli,M., Catta, M., Orlandini1,S., Amatiste, S., Bernardini, R.D., and Catillo, G.(2010). Effects of mastitis on buffalo milk quality. Asian-Aust. J. Anim. Sci., 23: 1319 - 1324

  36. Uallah, S., Ahmad, T., Billal, M.Q., Rahman, Z., Muhammad, G. and Rahman S.U. (2005). The effect of severity of mastitis on protein and fat contents of buffalo milk. Pakistan vet. J., 25:1-4.

  37. Varella Coelho, M.L., Santos Nascimento , J.D., Fagundes, P.C., Madureira, D.J., Oliveria, S.S., Vasconcelos de Paiva Brito, M.A. and Freire Bastos Mdo, C.(2007). Activty of Staphylococcus bacteriocins against Staphylococcus aureus and Streptococcus agalactiae involved in bovine mastitis. Research in Micro., 158: 625-630.

  38. Zhao, X. and Lacasse, P. (2008). Mammary tissue damage during bovine mastitis: Causes and control. J. Anim. Sci., 86: 57-65.

     
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