Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 43 issue 1 (march 2009) : 17-21

DETERMINATION OF SUITABLE CONCENTRATION OF CAFFEINE AND INCUBATION PERIOD FOR IN VITRO CAPACITATION OF BOVINE SPERMATOZOA

S. Singh, H.P. Gupta, A.K. Misra, M.S. Saxena
1College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand - 263 145, India

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Cite article:- Singh S., Gupta H.P., Misra A.K., Saxena M.S. (2024). DETERMINATION OF SUITABLE CONCENTRATION OF CAFFEINE AND INCUBATION PERIOD FOR IN VITRO CAPACITATION OF BOVINE SPERMATOZOA. Indian Journal of Animal Research. 43(1): 17-21. doi: .

ABSTRACT

In order to determine the optimum concentration of caffeine and incubation period for in vitro
capacitation of frozen bovine spermatozoa, washed spermatozoa were incubated in sperm-TALP
(Tyrode's albumin, lactate and pyruvate) having bovine serum albumin @ 6mg/ml along with
supplementation of caffeine @ 0, 5, 10 and 20 mM for 0, 30, 60 and 90 min. In vitro capacitation
was evaluated on the basis of hyperactive motility, head to head agglutination and acrosome
reaction of spermatozoa. The highest rate of capacitation was observed with 20 mM caffeine at
90 min. of incubation.

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