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Current IssueEditorial BoardOnline First ArticlesArchive

volume 43 issue 1 (march 2009) : 17-21

DETERMINATION OF SUITABLE CONCENTRATION OF CAFFEINE AND INCUBATION PERIOD FOR IN VITRO CAPACITATION OF BOVINE SPERMATOZOA

S
S. Singh
H
H.P. Gupta
A
A.K. Misra
M
M.S. Saxena
1College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand - 263 145, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Singh S., Gupta H.P., Misra A.K., Saxena M.S. (2025). DETERMINATION OF SUITABLE CONCENTRATION OF CAFFEINE AND INCUBATION PERIOD FOR IN VITRO CAPACITATION OF BOVINE SPERMATOZOA. Indian Journal of Animal Research. 43(1): 17-21. doi: .

ABSTRACT

In order to determine the optimum concentration of caffeine and incubation period for in vitro
capacitation of frozen bovine spermatozoa, washed spermatozoa were incubated in sperm-TALP
(Tyrode's albumin, lactate and pyruvate) having bovine serum albumin @ 6mg/ml along with
supplementation of caffeine @ 0, 5, 10 and 20 mM for 0, 30, 60 and 90 min. In vitro capacitation
was evaluated on the basis of hyperactive motility, head to head agglutination and acrosome
reaction of spermatozoa. The highest rate of capacitation was observed with 20 mM caffeine at
90 min. of incubation.

REFERENCES

  1. Agrawal, K.P. (1999). Indian J.Anim.Reprod., 20 (1):57-59.
  2. Austin, C.R. (1960). J.Reprod. Fertil., 1:310-311.
  3. Ax, R.L. et al. (1985). J.Dairy Sci., 68:387-390.
  4. Baranwal, A. and Maurya, S.N.(1999). XV Annual Convention of ISSAR and National Symposium on Biotechniques
  5. in Optimizing Fertility in Farm Animals, Ludhiana. February 10-12, 1999.
  6. Bird, J.M. et al. (1989). Theriogenology, 32:227-242.
  7. Chang, M.C. (1951). Nature, 168:997-998.
  8. Dott, H.M. and Walton, A.(1960). J. Reprod. Ferti., 1:350-354.
  9. E1-Menoufy, A.A. et al. (1986). Bulgarian J. Agric.Sci., 2:5-14.
  10. First, N.L. and Perish, J.J. (1987). J. Reprod. Fertil. Suppl., 34:151-165.
  11. Fraser, L.R. (1979). J. reprod. Fertil., 57:377-384.
  12. Gardon, J.C. et al. (2001). Anales de Veterinaria de Murcia, 17:19-27.
  13. Gordon, I. (1994). Laboratory Production of Cattle Embryo. CAB Internantional, Wallinghard.
  14. Harrison, R.A.P. (1996). Reprod. Fertil. Dev., 8:581-594.
  15. Haryama, H. et al. (1999). Reprod. Fertil. Dev., 11:193-9.
  16. Vol. 43, No. 1, 2009 21
  17. Haryama, H. et al. (2003). J. Androl., 24:1, 91-99.
  18. Iqbal, N. and Hunter, A.G. (1995). J. Dairy Sci., 78:91-102.
  19. Marquez, B. and Suarez, S.S. (2004). Biol. Reprod. 70:1626-1633.
  20. Park, C.K. et al. (1989). J. Reprod. Fertil., 86:2, 577-582.
  21. Parrish, J. et al. (1989). Biol. Reprod., 41:683-699.
  22. Pavlok, A. et al. (2001). Zygote, 9(1):25-38.
  23. Pomeroy, K.O. et al. (1988). J. Exp. Zool., 248:213-221.
  24. Serin, I. and Tekin, N. (2003). Ankara Universitesi-Veteriner-Fakultesi-Dergisi, 50(1):39-46.
  25. Snedecor, G.W. and Cochran, W.G. (1967). Statistical Methods. Iowa State University Press, Iowa, U.S.A.
  26. Tardif, S. et al. (2001). Biol. Reprod., 65:784-92.
  27. Watson, P.F. (1975). Vet. Rec., 97:12-15.
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Indian Journal of Animal Research
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    volume 43 issue 1 (march 2009) : 17-21

    DETERMINATION OF SUITABLE CONCENTRATION OF CAFFEINE AND INCUBATION PERIOD FOR IN VITRO CAPACITATION OF BOVINE SPERMATOZOA

    S
    S. Singh
    H
    H.P. Gupta
    A
    A.K. Misra
    M
    M.S. Saxena
    1College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand - 263 145, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Singh S., Gupta H.P., Misra A.K., Saxena M.S. (2025). DETERMINATION OF SUITABLE CONCENTRATION OF CAFFEINE AND INCUBATION PERIOD FOR IN VITRO CAPACITATION OF BOVINE SPERMATOZOA. Indian Journal of Animal Research. 43(1): 17-21. doi: .

    ABSTRACT

    In order to determine the optimum concentration of caffeine and incubation period for in vitro
    capacitation of frozen bovine spermatozoa, washed spermatozoa were incubated in sperm-TALP
    (Tyrode's albumin, lactate and pyruvate) having bovine serum albumin @ 6mg/ml along with
    supplementation of caffeine @ 0, 5, 10 and 20 mM for 0, 30, 60 and 90 min. In vitro capacitation
    was evaluated on the basis of hyperactive motility, head to head agglutination and acrosome
    reaction of spermatozoa. The highest rate of capacitation was observed with 20 mM caffeine at
    90 min. of incubation.

    REFERENCES

    1. Agrawal, K.P. (1999). Indian J.Anim.Reprod., 20 (1):57-59.
    2. Austin, C.R. (1960). J.Reprod. Fertil., 1:310-311.
    3. Ax, R.L. et al. (1985). J.Dairy Sci., 68:387-390.
    4. Baranwal, A. and Maurya, S.N.(1999). XV Annual Convention of ISSAR and National Symposium on Biotechniques
    5. in Optimizing Fertility in Farm Animals, Ludhiana. February 10-12, 1999.
    6. Bird, J.M. et al. (1989). Theriogenology, 32:227-242.
    7. Chang, M.C. (1951). Nature, 168:997-998.
    8. Dott, H.M. and Walton, A.(1960). J. Reprod. Ferti., 1:350-354.
    9. E1-Menoufy, A.A. et al. (1986). Bulgarian J. Agric.Sci., 2:5-14.
    10. First, N.L. and Perish, J.J. (1987). J. Reprod. Fertil. Suppl., 34:151-165.
    11. Fraser, L.R. (1979). J. reprod. Fertil., 57:377-384.
    12. Gardon, J.C. et al. (2001). Anales de Veterinaria de Murcia, 17:19-27.
    13. Gordon, I. (1994). Laboratory Production of Cattle Embryo. CAB Internantional, Wallinghard.
    14. Harrison, R.A.P. (1996). Reprod. Fertil. Dev., 8:581-594.
    15. Haryama, H. et al. (1999). Reprod. Fertil. Dev., 11:193-9.
    16. Vol. 43, No. 1, 2009 21
    17. Haryama, H. et al. (2003). J. Androl., 24:1, 91-99.
    18. Iqbal, N. and Hunter, A.G. (1995). J. Dairy Sci., 78:91-102.
    19. Marquez, B. and Suarez, S.S. (2004). Biol. Reprod. 70:1626-1633.
    20. Park, C.K. et al. (1989). J. Reprod. Fertil., 86:2, 577-582.
    21. Parrish, J. et al. (1989). Biol. Reprod., 41:683-699.
    22. Pavlok, A. et al. (2001). Zygote, 9(1):25-38.
    23. Pomeroy, K.O. et al. (1988). J. Exp. Zool., 248:213-221.
    24. Serin, I. and Tekin, N. (2003). Ankara Universitesi-Veteriner-Fakultesi-Dergisi, 50(1):39-46.
    25. Snedecor, G.W. and Cochran, W.G. (1967). Statistical Methods. Iowa State University Press, Iowa, U.S.A.
    26. Tardif, S. et al. (2001). Biol. Reprod., 65:784-92.
    27. Watson, P.F. (1975). Vet. Rec., 97:12-15.
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