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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 11 (november 2019) : 1497-1503,   Doi: 10.18805/ijar.B-3670

Molecular epidemiology and phylogenetic analysis of foot and mouth disease virus type ‘O’ and evaluation of its carrier state in cattle of Assam by Real time PCR

S
Sangeeta Baro
K
Krishna Sharma
B
Biswajyoti Borah
S
Shantanu Tamuly
P
P. Deka
P
Prince Boro
S
S.K. Das
1Department Of Microbiology, College Of Veterinary Science, Khanapara-781 022, Guwahati, India.
Cite article:- Baro Sangeeta, Sharma Krishna, Borah Biswajyoti, Tamuly Shantanu, Deka P., Boro Prince, Das S.K. (2018). Molecular epidemiology and phylogenetic analysis of foot and mouth disease virus type ‘O’ and evaluation of its carrier state in cattle of Assam by Real time PCR. Indian Journal of Animal Research. 53(11): 1497-1503. doi: 10.18805/ijar.B-3670.

ABSTRACT

The molecular epidemiological study of foot-and-mouth disease virus (FMDV) has been carried out from different outbreaks in Assam the present study is based on the nucleotide sequencingof circulating FMDV serotype. The samples were subjected to sandwich ELISA, multiplex-PCR and molecular phylogeny to identify the type species. The phylogenetic analysis of virus sequence revealed similarity with theBangladesh isolates in the major branching pattern. The serotype ‘O’has found to be dominant and responsible for most of the recentoutbreaks.Thepersistence of serotype ‘O’ and cytokines expression of IL-1á, IL-1â, IFN-á, TNF-á in blood of recovered animals were done by Real time PCR. The findings indicated that IL-1á, IFN-á and TNF-á genes were up-regulated upto 3 months post infection but IL-1â found to be down regulated with progression of recovery. The present study thus supports that real-time PCR is a powerful technique for reliable detection of persistent FMDV in recovered animals.

REFERENCES

  1. Alexandersen, S., Quan, M., Murphy, C., Knight J. and Zhang, Z (2003). Studies of quantitative parameters of virus excretion and transmission in pigs and cattle experimentally infected with foot-and-mouth disease virus. J. Comp. Pathol.129: 268-282
  2. Bachrach, H. L. (1968). Foot-and-mouth disease. Annu. Rev. Microbiol. 22:201-244.
  3. Barker, I. K., VanDreumel, A. A. and Palmer, N. (1993). The alimentary system. Pathology of Domestic Animals pp 141–44. (Ed.) Jubb KVF, Kennedy PC, and Palmer N, 4th edn, vol. 2. Academic Press, San Diego,
  4. Belsham, G. J. (2005). Translation and replication of FMDV RNA. Current Topics in Microbiol. Immunol. 288: 43–70.
  5. Das, L. (2013). Persistence of foot and mouth disease virus type O in cattle in Assam. M.V.Sc thesis. College of Veterinary Science, AAU, Khanapara, Guwahati-22.
  6. Doel, T. R., Williams, L. and Barnett, P. V. (1994). Emergency vaccination against foot-and-mouth disease: rate of development of immunity and its implications for the carrier state. Vaccine. 12:592–600.
  7. Giridharan, P., Hemadri, D., Tosh, C., Sanyal, A. and Bandyopadhyay, S. K.; (2005). Development and evaluation of a multiplex PCR for differentiation of foot-and-mouthdisease virus strains native to India. J. Virol. Meth. 126:1–11.
  8. Grubman, M. J. and Baxt B. (2004). Foot-and-mouth disease. Clin. Microbiol. Rev. 2: 465–493.
  9. Mackay, D.K., Forsyth, M.A., Davies, P.R. and Salt, J.S. (1998). Antibody to the nonstructural proteins of foot-and-mouth disease virus in vaccinated animals exposed to infection. Vet. Q.20(Suppl. 2):9–11
  10. R-CORE TEAM (2016): A Language and environment for statistical computing (Version 3.3.2)Vienna, Austria.
  11. Rufael, T. A., Catley, A., Bogale, M., Sahle and Shiferaw, Y. (2008). Foot and mouth disease in the Borana pastoral system, southern Ethiopia and implications for livelihoods and international trade. Tropical Animal Health and Production. 40: 29– 38.
  12. Salt, J. S. (1993). The carrier state in foot and mouth disease-animmunological review. British Vet. J. 149: 207–23
  13. Salt, J. S. (2004). Persistence of foot-and-mouth disease virus In: Foot and Mouth Disease Current Perspectives Wymondham, [Sobrino F, Domingo E, editors]: Horizon Bioscience. UK. pp. 103–143.
  14. Suttmoller, P. and Gaggero, S. (1965). A. Foot-and mouth diseases carriers. Vet Rec. 77: 968–969.
  15. Wilkins, C and Gale, M. Jr. ( 2010). Recognition of viruses by cytoplasmic sensors. Curr. Opin. Immunol. 22:41–47
  16. Zinnah, M.A, Islam M.T., Rahman, M.M., Hossain, M.T, Bari, M.R. Haque, M.H., Khan, M.S.R. and Islam, M.A. (2010): Standardization of multiplex reverse transcriptase polymerase chain reaction and typing of foot and mouth disease virus prevalent in Bangladesh. Bangl. J. Vet. Med 8(2):149-155
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    Research Article

    volume 53 issue 11 (november 2019) : 1497-1503,   Doi: 10.18805/ijar.B-3670

    Molecular epidemiology and phylogenetic analysis of foot and mouth disease virus type ‘O’ and evaluation of its carrier state in cattle of Assam by Real time PCR

    S
    Sangeeta Baro
    K
    Krishna Sharma
    B
    Biswajyoti Borah
    S
    Shantanu Tamuly
    P
    P. Deka
    P
    Prince Boro
    S
    S.K. Das
    1Department Of Microbiology, College Of Veterinary Science, Khanapara-781 022, Guwahati, India.
    Cite article:- Baro Sangeeta, Sharma Krishna, Borah Biswajyoti, Tamuly Shantanu, Deka P., Boro Prince, Das S.K. (2018). Molecular epidemiology and phylogenetic analysis of foot and mouth disease virus type ‘O’ and evaluation of its carrier state in cattle of Assam by Real time PCR. Indian Journal of Animal Research. 53(11): 1497-1503. doi: 10.18805/ijar.B-3670.

    ABSTRACT

    The molecular epidemiological study of foot-and-mouth disease virus (FMDV) has been carried out from different outbreaks in Assam the present study is based on the nucleotide sequencingof circulating FMDV serotype. The samples were subjected to sandwich ELISA, multiplex-PCR and molecular phylogeny to identify the type species. The phylogenetic analysis of virus sequence revealed similarity with theBangladesh isolates in the major branching pattern. The serotype ‘O’has found to be dominant and responsible for most of the recentoutbreaks.Thepersistence of serotype ‘O’ and cytokines expression of IL-1á, IL-1â, IFN-á, TNF-á in blood of recovered animals were done by Real time PCR. The findings indicated that IL-1á, IFN-á and TNF-á genes were up-regulated upto 3 months post infection but IL-1â found to be down regulated with progression of recovery. The present study thus supports that real-time PCR is a powerful technique for reliable detection of persistent FMDV in recovered animals.

    REFERENCES

    1. Alexandersen, S., Quan, M., Murphy, C., Knight J. and Zhang, Z (2003). Studies of quantitative parameters of virus excretion and transmission in pigs and cattle experimentally infected with foot-and-mouth disease virus. J. Comp. Pathol.129: 268-282
    2. Bachrach, H. L. (1968). Foot-and-mouth disease. Annu. Rev. Microbiol. 22:201-244.
    3. Barker, I. K., VanDreumel, A. A. and Palmer, N. (1993). The alimentary system. Pathology of Domestic Animals pp 141–44. (Ed.) Jubb KVF, Kennedy PC, and Palmer N, 4th edn, vol. 2. Academic Press, San Diego,
    4. Belsham, G. J. (2005). Translation and replication of FMDV RNA. Current Topics in Microbiol. Immunol. 288: 43–70.
    5. Das, L. (2013). Persistence of foot and mouth disease virus type O in cattle in Assam. M.V.Sc thesis. College of Veterinary Science, AAU, Khanapara, Guwahati-22.
    6. Doel, T. R., Williams, L. and Barnett, P. V. (1994). Emergency vaccination against foot-and-mouth disease: rate of development of immunity and its implications for the carrier state. Vaccine. 12:592–600.
    7. Giridharan, P., Hemadri, D., Tosh, C., Sanyal, A. and Bandyopadhyay, S. K.; (2005). Development and evaluation of a multiplex PCR for differentiation of foot-and-mouthdisease virus strains native to India. J. Virol. Meth. 126:1–11.
    8. Grubman, M. J. and Baxt B. (2004). Foot-and-mouth disease. Clin. Microbiol. Rev. 2: 465–493.
    9. Mackay, D.K., Forsyth, M.A., Davies, P.R. and Salt, J.S. (1998). Antibody to the nonstructural proteins of foot-and-mouth disease virus in vaccinated animals exposed to infection. Vet. Q.20(Suppl. 2):9–11
    10. R-CORE TEAM (2016): A Language and environment for statistical computing (Version 3.3.2)Vienna, Austria.
    11. Rufael, T. A., Catley, A., Bogale, M., Sahle and Shiferaw, Y. (2008). Foot and mouth disease in the Borana pastoral system, southern Ethiopia and implications for livelihoods and international trade. Tropical Animal Health and Production. 40: 29– 38.
    12. Salt, J. S. (1993). The carrier state in foot and mouth disease-animmunological review. British Vet. J. 149: 207–23
    13. Salt, J. S. (2004). Persistence of foot-and-mouth disease virus In: Foot and Mouth Disease Current Perspectives Wymondham, [Sobrino F, Domingo E, editors]: Horizon Bioscience. UK. pp. 103–143.
    14. Suttmoller, P. and Gaggero, S. (1965). A. Foot-and mouth diseases carriers. Vet Rec. 77: 968–969.
    15. Wilkins, C and Gale, M. Jr. ( 2010). Recognition of viruses by cytoplasmic sensors. Curr. Opin. Immunol. 22:41–47
    16. Zinnah, M.A, Islam M.T., Rahman, M.M., Hossain, M.T, Bari, M.R. Haque, M.H., Khan, M.S.R. and Islam, M.A. (2010): Standardization of multiplex reverse transcriptase polymerase chain reaction and typing of foot and mouth disease virus prevalent in Bangladesh. Bangl. J. Vet. Med 8(2):149-155
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