INDUCED MUTAGENESIS AND IN VITRO CELL SELECTION FOR RESISTANCE AGAINST ALTERNARIA BLIGHT IN BRASSICA NAPUS L.

Article Id: ARCC401 | Page : 369 - 373
Citation :- INDUCED MUTAGENESIS AND IN VITRO CELL SELECTION FOR RESISTANCE AGAINST ALTERNARIA BLIGHT IN BRASSICA NAPUS L..Indian Journal Of Agricultural Research.2012.(46):369 - 373
Munish Sharma, S.K. Gupta and Aditya Pratap*
Address : Department of Plant Breeding and Genetics, Sher e Kashmir University of Agriculture and Technology -Jammu, Chatha, Jammu-180 009, India

Abstract

The percentage of survival of cell lines of Brassica napus was worked out against the different physical (gamma rays) and chemical (EMS) mutagens. Half of the regenerated calli of var. DGS 1 were subjected to three doses viz., 80 Gy, 120 Gy and 160 Gy of gamma rays. However, the treatment did not show any adverse effect on the growth of calli at any of the above doses, though it significantly affected the regeneration percentage. On the other hand, treatment of calli with 3 per cent of EMS for 90 minutes resulted in 100 per cent lethality, whereas, 30 and 60 minutes duration could produce 20 per cent and 15 per cent surviving calli, respectively. Calli treated with two mutagens, viz. gamma rays and EMS were subjected to different concentrations of culture filtrate of Alternaria brassicicola. Survival of the calli decreased with the increase in the concentration of culture filtrate. Among these, the selective dose of culture filtrate was found to be 10 per cent and the cell survival of calli treated with two mutagens at this dose of culture filtrate varied between 1.88 – 5.76 per cent in different treatments. The study resulted in regeneration of A. brassicicola resistant plants from selected and unselected calli.

Keywords

Brassica napus Alternaria Brassicicola Cell selection Induced mutagenesis Gamma rays EMS.

References

  1. Cambecedes, J ; Duron , M ; Decourtye, L. and Jalouzot, R.(1992). Methodology of in vitro gamma rays irradiations from Lonicera species, mutant description and biochem. characterization. Acta Horticulturae. 320 : 119-126.
  2. Chopra, V. L. and Sharma, R. P. (1985). Induced mutations in crop improvement. In: Genetic Manipulation for Crop Improvement. V. L. Chopra (ed). Oxford and IBH Pub. Co. New Delhi.
  3. Conn, K.L; Tewari, J. P. and Dahiya, J. S. (1988). Resistance to Alternaria brassicae and Phytoalexin- elicitation in Rapeseed and Other crucifers. Plant Science. 56 :21-25.
  4. Gomez, K. A. and Gomez, A. A. (1983). Statistical Procedures for Agricultural Research. Second Ed. John Wiley and Sons. New York, USA.
  5. Gupta, S. K. and Pratap, A. (2007). History, Origin and Evolution in Oilseed Brassicas. Advan. \Botan. Res. 45 : 2-17.
  6. Hussain, S. N. And Hussain, M. (1996). In vitro morphogenetic responses of Peltophorum petrocarpum following phytohormonal and gamma-irradiation treatment. In: Plant Tissue Culture. Islam A.S.(ed). Oxford and IBH Pub. Co. Pvt. Ltd., New Delhi, pp. 33-36.
  7. Jan, B; Kang, F. Y. and Peter Pauls, K. (1993). In vitro selection for disease/toxins resistance. In: Plant cell cultures: A practical approach (2nd ed). [Dixon R. A. and Gonzales R. A. (eds)]. Oxford University Press. Oxford.
  8. Kolte, S. J; Awasthi, R. P. and Vishwanath. (1987). Assesment of yield losses due to Alternaria blight in rapeseed and mustard. Indian Phytopath. 40 (2): 209-211.
  9. Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 15 : 473 - 479.
  10. Pratap, A; Gupta, S. K. and Sharma, M. (2008). Genetic amelioration of crop Brassica through intergeneric hybridization using Diplotaxis. Presented in seminar on Physiological and Biotechnological Approaches to Improve Plant Productivity on 15-17 march 2008 at Centre for Plant Bitechnology, Department of Science and Technology, Haryana. CCSHAU, Hissar.
  11. Rai, B; Gupta, S. K. and Pratap, A. (2007). Breeding Methods in Oilseed Brassicas. Advances in Botan. Res. 45 : 2-17.
  12. Riker, A. J. and Riker, R. S. (1936). Introduction to Research on Plant Diseases. John S. Swift Co. St. Loius.
  13. Ritchie, D. F; Werner, D. J. and Hammerschlag. F. A. (1993). Field evaluation of tissue culture derived Peach trees for susceptibility to bacteria (Xamthomonas campestris pv. Pruni). Acta Horticulturae. 336: 155-63.
  14. Sareen, P. K; Sharma, D.R; Vijay Kumar, N.K and Choudhury, J.B.(1982). Leaf merophyll tissue as a mutagen test System. Indian J. Exper. Biol 20 : 260-261.
  15. Shahin, E.A. and Spivey, R. (1986). A single dominent gene for Fusarium wilt resistance in protoplast derived tomato plants. Theor. Appl. Genet. 73 : 164- 169.
  16. Sigareva, M. A. and Earle, E. D. (1998). Camalexin induction in intertribal somatic hybrids between Camelina sativa and rapid cycling Brassica oleracea. Theor. Appl. Genet. 98 : 164-170.
  17. Werner, D.J..Ritchie, D.F., Cain, D. W. and Zehr, E. I. (1986). Susceptibility of Peaches, Nectarines. Plant introductions and other Prunus species to bacterial spot. Hort. Sci. 21 : 127-30.
  18. Westman, A. L; Kresovich, S. and Dickson, M. H. (1999). Regional variation in Brassica nigra and other weedy crucifers for disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris. Euphytica. 106: 253-259.

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