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Current IssueEditorial BoardOnline First ArticlesArchive

volume 46 issue 4 (december 2012) : 360 - 364

CRITICAL ABSORPTION LIMIT OF NITRATE AND OPTIMIZING ESTIMATION METHODOLOGY FOR SOIL SOLUTIONS AND EXTRACTS USING UV-VIS SPECTROSCOPY

L
L.K. Prasad*
K
K. Rajan
1Icar Research complex for Eastern Region, B.V. Campus, Patna-800 014 India

  • Submitted|

  • First Online |

  • doi

Cite article:- Prasad* L.K., Rajan K. (2025). CRITICAL ABSORPTION LIMIT OF NITRATE AND OPTIMIZING ESTIMATION METHODOLOGY FOR SOIL SOLUTIONS AND EXTRACTS USING UV-VIS SPECTROSCOPY. Indian Journal of Agricultural Research. 46(4): 360 - 364. doi: .

ABSTRACT

Absorption behavior of nitrate and estimation methodology has been studied using different types of samples and UV wavelengths in order to optimize the use of samples and wavelengths to ensure better results. Absorption was highest in 190-310 nm range scanning compared to the single wavelength (210) or combination wavelengths (210+301 nm). Linearity in absorption was up to 40 mg/ml nitrate nitrogen in case of 190-310 nm scan. The wavelengths identified for nitrate in soil leachate samples for maximum absorption was combination of 210+301 nm wavelengths, whereas, wavelength scan from 190 to 310 nm was found better for soil extracts and nutrient solutions. Molar absorption coefficient was highest in case of 190-310 nm wavelength scan region for aqueous solutions. The critical absorption value of nitrate was found as 3.35 at different treatment wavelengths. Using these combination of treatment wavelengths to suitable sample types could yield better absorption results and with a little cost under laboratory conditions.

REFERENCES

  1. Agarwal Y.K, and Mehd,G.D. (1983) Solvent extraction and spectrophotometric determination of some toxic metal ions. Solvent Rev.Anal.Chem, 6 :185.
  2. Bremner, J.M, and Keeney, D.R. (1966) Determination and isotope ratio analysis of different forms of nitrogen in soils: 3.Exchangeable ammonium, nitrate and nitrate by extraction-distillation methods. Soil Sci.Soc.Am.Proc, 30: 577-582.
  3. Cate, R.B.Jr and Nelson, L.A. (1971) A simple statistical procedure for partitioning soil test correlation data in two classes. Soil Sci. Soc. of Am. Proc. 35:658-659.
  4. Cawse, P.A. (1967) The determination of nitrate in soil solutions by ultra violet spectrophotometry Analyst, 92: 311-315.
  5. Cottrell, C.T, Irish, D, Masters, V.M, Steward, J. E. (1985) Introduction to Ultraviolet and Visible Spectrophotometry; 2nd edn; Pye Unicam Ltd: Cambridge.
  6. Grambell, R.P, Gillium, J.W, and Weed, S.B (1975) Denitrification in sub soils of the North Carolina Coastal Plain as affected by soil drainage J.Environ.Qual., 4:311-316.
  7. Kim, D.W, Chae, J.C, and Kim, S.W. (2001) Effect of rice cultural practices on water percolation, irrigation requirement, and nitrogen leaching under lysimeter condition. Korean J. Crop Sci., 46: 1, 6-11.
  8. Langergraber.G, Fleischmann.N, and Hofstadter.H, (2003) A multivariate calibration procedure for UV/VIS spectrometric quantification of organic matter and nitrate in wastewater. Wat. Sci. Tech., 47: 2, 65-71.
  9. Mac Donald,J.C, and Haddad, L ,(1970) Ultra violet spectrophotometric determination of nitrite-nitrate in KOH. Environ.Sci.Technol, 4:676-678.
  10. Norman,R.J, and Stucki, J.W. (1981) The determination of nitrate and nitrite in soil extracts by ultraviolet spectrometry. Soil Sci. Soc of Am.J, 45: 347-353.
  11. Wetters,J.H, and Uglum,K.L. (1970) Direct spectro-photometric simultaneous determination of nitrite and nitrate in the ultraviolet. Anal.Chem, 42: 335-340.
  12. Zhu, J.G, G.Liu, Y. Han, Y.L. Zhang, and G.X. Xing (2003) Nitrate distribution and denitrification in the saturated zone of paddy field under rice/wheat rotation . Chemosphere, 50: 6:725-732.
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    volume 46 issue 4 (december 2012) : 360 - 364

    CRITICAL ABSORPTION LIMIT OF NITRATE AND OPTIMIZING ESTIMATION METHODOLOGY FOR SOIL SOLUTIONS AND EXTRACTS USING UV-VIS SPECTROSCOPY

    L
    L.K. Prasad*
    K
    K. Rajan
    1Icar Research complex for Eastern Region, B.V. Campus, Patna-800 014 India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Prasad* L.K., Rajan K. (2025). CRITICAL ABSORPTION LIMIT OF NITRATE AND OPTIMIZING ESTIMATION METHODOLOGY FOR SOIL SOLUTIONS AND EXTRACTS USING UV-VIS SPECTROSCOPY. Indian Journal of Agricultural Research. 46(4): 360 - 364. doi: .

    ABSTRACT

    Absorption behavior of nitrate and estimation methodology has been studied using different types of samples and UV wavelengths in order to optimize the use of samples and wavelengths to ensure better results. Absorption was highest in 190-310 nm range scanning compared to the single wavelength (210) or combination wavelengths (210+301 nm). Linearity in absorption was up to 40 mg/ml nitrate nitrogen in case of 190-310 nm scan. The wavelengths identified for nitrate in soil leachate samples for maximum absorption was combination of 210+301 nm wavelengths, whereas, wavelength scan from 190 to 310 nm was found better for soil extracts and nutrient solutions. Molar absorption coefficient was highest in case of 190-310 nm wavelength scan region for aqueous solutions. The critical absorption value of nitrate was found as 3.35 at different treatment wavelengths. Using these combination of treatment wavelengths to suitable sample types could yield better absorption results and with a little cost under laboratory conditions.

    REFERENCES

    1. Agarwal Y.K, and Mehd,G.D. (1983) Solvent extraction and spectrophotometric determination of some toxic metal ions. Solvent Rev.Anal.Chem, 6 :185.
    2. Bremner, J.M, and Keeney, D.R. (1966) Determination and isotope ratio analysis of different forms of nitrogen in soils: 3.Exchangeable ammonium, nitrate and nitrate by extraction-distillation methods. Soil Sci.Soc.Am.Proc, 30: 577-582.
    3. Cate, R.B.Jr and Nelson, L.A. (1971) A simple statistical procedure for partitioning soil test correlation data in two classes. Soil Sci. Soc. of Am. Proc. 35:658-659.
    4. Cawse, P.A. (1967) The determination of nitrate in soil solutions by ultra violet spectrophotometry Analyst, 92: 311-315.
    5. Cottrell, C.T, Irish, D, Masters, V.M, Steward, J. E. (1985) Introduction to Ultraviolet and Visible Spectrophotometry; 2nd edn; Pye Unicam Ltd: Cambridge.
    6. Grambell, R.P, Gillium, J.W, and Weed, S.B (1975) Denitrification in sub soils of the North Carolina Coastal Plain as affected by soil drainage J.Environ.Qual., 4:311-316.
    7. Kim, D.W, Chae, J.C, and Kim, S.W. (2001) Effect of rice cultural practices on water percolation, irrigation requirement, and nitrogen leaching under lysimeter condition. Korean J. Crop Sci., 46: 1, 6-11.
    8. Langergraber.G, Fleischmann.N, and Hofstadter.H, (2003) A multivariate calibration procedure for UV/VIS spectrometric quantification of organic matter and nitrate in wastewater. Wat. Sci. Tech., 47: 2, 65-71.
    9. Mac Donald,J.C, and Haddad, L ,(1970) Ultra violet spectrophotometric determination of nitrite-nitrate in KOH. Environ.Sci.Technol, 4:676-678.
    10. Norman,R.J, and Stucki, J.W. (1981) The determination of nitrate and nitrite in soil extracts by ultraviolet spectrometry. Soil Sci. Soc of Am.J, 45: 347-353.
    11. Wetters,J.H, and Uglum,K.L. (1970) Direct spectro-photometric simultaneous determination of nitrite and nitrate in the ultraviolet. Anal.Chem, 42: 335-340.
    12. Zhu, J.G, G.Liu, Y. Han, Y.L. Zhang, and G.X. Xing (2003) Nitrate distribution and denitrification in the saturated zone of paddy field under rice/wheat rotation . Chemosphere, 50: 6:725-732.
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