volume 36 issue 1 (march 2002) : 39 - 43
DETECTION OF XANTHOMONAS'VESICATORIA IN TOMATO SEEDS BY ENZYME LINKED IMMUNO SORBENT ASSAY (ELISA)
1Department of Plant Pathology,
GKVK, Bangalore - 560065, India
ABSTRACT
Rapid detection of Xanthomonas vesicatoria in the tomato seeds is very essential for preventing dissemination of bacteria and imposition of strict quarantines. Application of serodiagnostic tools will help to larger seed samples to avoid possible interceptions of disease. Hence, ELISA was applied with slight modification by using tetra methyl benzidine substrate with reduced duration of incubation. This technique was found to be more effective and rapid in detection of Xanthomonas vesicatoria in naturally infected seeds. The efficiency was better compared with the dilution planting methods with higher extinction values in seed extracts containing higher number of seeds (0.235–0.237). The pathogen population was detected was 318 colony forming unit/ml. ELISA can be applied as effective tool up to the detectable limit of ten per cent.
KEYWORDS
REFERENCES
- Alvarez, A.M. and Lou, K. (1985). PI. Disease, 69: 1082-1086.
- Avrameas, S. and Ternyek. T. (1971). Immuno ChemistJy. 8: 1175-1179.
- Barzic, M.R. and Trigalet, A.. (1982). Agromonie., 2:389-398.
- Bradford, M.M. (1976). Anal, Biochemistry, 72: 249-254.
- Gardner, M.W. and Kendrick, J.B., (1921). J. Agric. Rp.s. 21:123-156.
- Gardner, M.W. and Kendrick, J.B. (1923). Phytopathology, 13:308-314.
- Khan. A.N.A. (1996). Proc. International Conference on Plant Pathogenic Bacteria.
- Sheppered, J.w. (1983). SeedSci. Techno/., 11:561-567.
- Van Vuurde, J.w.L. (1984a) In: Arst Int. Workshop ofSeed Bacterial, Angern., pp. 15-221 ISTA, Zurich.
- Van Vuurde, J.w.L. (1984 b). In: Arst Int. Workshop ofSeed Bacteria/. Angern., pp. 42-44, Zurich.
- Vruggink, H. and Geesteranus Mass, H.P. (1975). Potato Res. 18:546-555.
- 43
Disclaimer :
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Copyright :
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Published In
Indian Journal of Agricultural Research
Article Metrics
0
Views
Citations
Reviewed By
In this Article
APC
APC cover the cost of turning a manuscript into a published manuscript through peer-review process, editorial work as well as the cost of hosting, distributing, indexing and promoting the manuscript.
Publish With US
Submit your manuscript through user friendly platform and acquire the maximum impact for your research by publishing with ARCC Journals.
Become a Reviewer/Member
Join our esteemed reviewers panel and become an editorial board member with international experts in the domain of numerous specializations.
Open Access
Filling the gap between research and communication ARCC provide Open Access of all journals which empower research community in all the ways which is accessible to all.
Products and Services
We provide prime quality of services to assist you select right product of your requirement.
Support and Policies
Finest policies are designed to ensure world class support to our authors, members and readers. Our efficient team provides best possible support for you.
