Indian Journal of Agricultural Research

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Indian Journal of Agricultural Research, volume 36 issue 1 (march 2002) : 39 - 43

DETECTION OF XANTHOMONAS'VESICATORIA IN TOMATO SEEDS BY ENZYME LINKED IMMUNO SORBENT ASSAY (ELISA)

M.R. Ravikumar, A.N.A. Khan
1Department of Plant Pathology, GKVK, Bangalore - 560065, India

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Cite article:- Ravikumar M.R., Khan A.N.A. (2024). DETECTION OF XANTHOMONAS'VESICATORIA IN TOMATO SEEDS BY ENZYME LINKED IMMUNO SORBENT ASSAY (ELISA). Indian Journal of Agricultural Research. 36(1): 39 - 43. doi: .

ABSTRACT

Rapid detection of Xanthomonas vesicatoria in the tomato seeds is very essential for preventing dissemination of bacteria and imposition of strict quarantines. Application of serodiagnostic tools will help to larger seed samples to avoid possible interceptions of disease. Hence, ELISA was applied with slight modification by using tetra methyl benzidine substrate with reduced duration of incubation. This technique was found to be more effective and rapid in detection of Xanthomonas vesicatoria in naturally infected seeds. The efficiency was better compared with the dilution planting methods with higher extinction values in seed extracts containing higher number of seeds (0.235–0.237). The pathogen population was detected was 318 colony forming unit/ml. ELISA can be applied as effective tool up to the detectable limit of ten per cent.

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