Indian Journal of Agricultural Research

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Indian Journal of Agricultural Research, volume 43 issue 3 (september 2009) : 223-226

LIGNOCELLULOLYTIC ENZYME ACTIVITIES AND SUBSTRATE DEGRADATION BY VOLVARIELLA VOLVACEA, THE PADDY STRAW MUSHROOM/CHINESE MUSHROOM

Madhu Choudhary, Shashi Dhanda, Shammi Kapoor, Giridhar Soni
1Department of Microbiology Punjab Agricultural University, Ludhiana-141 004, India

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Cite article:- Choudhary Madhu, Dhanda Shashi, Kapoor Shammi, Soni Giridhar (2024). LIGNOCELLULOLYTIC ENZYME ACTIVITIES AND SUBSTRATE DEGRADATION BY VOLVARIELLA VOLVACEA, THE PADDY STRAW MUSHROOM/CHINESE MUSHROOM. Indian Journal of Agricultural Research. 43(3): 223-226. doi: .

ABSTRACT

All the six strains of Volvariella volvacea were investigated for their lignocellulolytic
potential. The strain VvS-4 and VvS-5 found to have maximum Carboxymethyl
cellulase (CMCase) and Filter paperase (FPase) activity. The cellobiase activity was
comparatively much higher than CMCase and FPase activity. Thus strain VvS-4 was
found to be the best strain in terms of CMCase, FPase and cellobiase activity. The
strain VvS-5 gave almost similar results except cellobiase activity. The xylanase activity
was found maximum in strain VvS-4 i.e. 147 IU/100ml and lesser in VvS-5 (108 IU/
100ml). Xylanase activity was higher than cellulase activity when it was correlated
with enzyme activities. None of the cultures showed a detectable laccase activity.
The maximum cellulose and hemicellulose degradation was found in VvS-4 and VvS-
5 (21.86% and 21.48%; 44.80% and 46.13% respectively) compared to all other
strains of V. volvacea and the minimum degradation in fresh paddy straw and control
(uninoculated). The pattern of cellulose and hemicellulose degradation was similar
in VvS-4 and VvS-5.

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