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Current IssueEditorial BoardOnline First Articles
Indian Journal of
Agricultural Research
Print ISSN: 0367-8245
Online ISSN: 0976-058X
NASS Rating
5.60
SJR
0.217
CiteScore
0.595

Chief Editor:
V. Geethalakshmi
Tamil Nadu Agricultural University Coimbatore, INDIA
Frequency:Monthly
Indexing:
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Go...

Indian Journal of Agricultural Research, volume 45 issue 2 (june 2011) : 140 - 145

IN VITRO PLANT REGENERATION OF TRICHOSANTHES DIOICA ROXB. CV. HILLI FROM NODAL EXPLANTS

Rashmi Komal
1Tissue Culture Laboratory, Department of Botany Patna University, Patna- 800 006, India.

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Cite article:- Komal Rashmi (2025). IN VITRO PLANT REGENERATION OF TRICHOSANTHES DIOICA ROXB. CV. HILLI FROM NODAL EXPLANTS. Indian Journal of Agricultural Research. 45(2): 140 - 145. doi: .

ABSTRACT

An efficient protocol was established for in vitro plant regeneration from nodal explants of Trichosanthes dioica Roxb. cv. hilli. Nodal segments were cultured on semisolid MS basal medium supplemented with different concentrations and combinations of cytokinins (BAP and Kinetin) and auxin (NAA) for direct shoot regeneration. Among the various concentrations of growth regulators used, MS supplemented with 2.0mg/l BAP + 0.3mg/l NAA showed highest percentage of shoot regeneration (92.74%) and maximum number of shoots per explants (4.00 ± 0.74) from lower nodal segment used as explants after four weeks of culture. The elongated shoots showed best rooting within seven to eight days after sub culturing in half strength MS supplemented with 1.0mg/l IBA. IBA proved to be better than IAA for efficient rooting. The in vitro raised plantlets were successfully established in earthen pots filled with soil, sand and farmyard mixture, and 75% of them survived in the field conditions.

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