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ASSESSMENT OF GENETIC DIVERSITY AMONG FINGER MILLET GENOTYPES USING RAPD MARKERS

Article Id: ARCC1057 | Page : 112 - 118
Citation :- ASSESSMENT OF GENETIC DIVERSITY AMONG FINGER MILLET GENOTYPES USING RAPD MARKERS.Indian Journal of Agricultural Research.2010.(44):112 - 118
S. Das and R.C. Misra
Address : Department of Plant Breeding and Genetics, College of Agriculture, O.U.A.T, Bhubaneswar-751 003, India

Abstract

Genetic diversity among fifteen finger millet genotypes was estimated using RAPD markers.
Out of the initial twenty-five random primers, nine RAPD primers were found to be highly
reproducible and produced a total of 60 loci of which 51 loci were polymorphic. Primer OPC18
amplified highest number of polymorphic bands. Percentage of polymorphism varied from 50%
to 100% with an average of 80.8%. Polymorphism information content (PIC) value of the primers
ranged from 0.17 to 0.38 and the resolving power (Rp) varied from 2.66 to 12.06. Primers OPN16
and OPD8 exhibited high Rp along with high PIC value. The coefficient of similarity in banding
pattern among genotypes ranged from0.42 to 0.89. The genotypes OEB 65 & HR 374 and BM
107 & SRS 2 showed high genetic similarity of 0.89 and 0.87, respectively indicating low genetic
diversity and also showed similarity in growth traits. DM 7 and AKP 2 showed the lowest similarity
of 0.42 indicating them to be quite diverse. Cluster analysis by UPGMA method grouped the
fifteen finger millet genotypes into one major cluster of eight genotypes and five minor clusters
indicating presence of genetic diversity among the genotypes at molecular level.

Keywords

Finger millet Genetic diversity RAPD markers

References

  1. Devanshi, S.A.K. et al. (2007). Indian J. Genet., 67: 121-127.
  2. 118 INDIAN JOURNAL OF AGRICULTURAL RESEARCH
  3. Doyle, J.J. and Doyle, J.L. (1990). Focus, 12: 13-15.
  4. Fernandez, M. et al. (2002). Theor. Appl. Genet., 104: 845-851.
  5. Hilu, K.W. (1995). Genome, 38: 232-238.
  6. Nei, M. and Li, W.H. (1979). Proc. Natl. Acad. Sci., USA , 76:5269-5273.
  7. Parani, M. et al. (2001). Genome, 44: 495-499.
  8. Prevost, A. and Wilkinson, M. (1999). Theor. Appl. Genet., 98: 107-112.
  9. Rachie, K. O. (1975). The Millets: Importance, Utilization and Outlook. International Crops Research Institute for the
  10. Semi-Arid Tropics, Hyderabad, India, pp.63.
  11. Rohlf, F.J. (1995). NTSYS-PC Numerical Taxonomy and Multivariate Analysis System,Version 1.80. Exter
  12. Software,Setauket, New York.
  13. Roldan-Ruiz, I. et al. (2000). Mol. Breed., 6:125-134.
  14. Russell, J. et al. (1997). Theor. Appl. Genet., 95: 714-722.
  15. Salimath, S.S. et al. (1995). Genome, 38: 757-763.
  16. Wilkie, S.E. et al. (1993). Theor. Appl. Genet.,86: 497-504.
  17. Yu, L.X. and Nguyen, H.T. (1994). Theor. Appl. Genet., 87: 668-672.

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