Article Id: ARCC072 | Page : 471-479
A. Manivannan*1, A.K.Chhabra and Someer Nimbal
Address : Department of Genetics and Plant Breeding College of Agriculture, CCS HAU, Hisar-125004, India


Isozymes are the product of the gene expression and it is a useful basic analytical tool to study the molecular characterization. As an important genetic marker, it is widely used to detect the inheritance and variance among different species or different cultivars within the species at the molecular level. Superoxide dismutase (SOD) is one enzyme of the antioxidase system and generally exists in all kinds of plants. SODs are ubiquitous metalloenzymes that catalyze dismutation of superoxide radicals and prevents organisms from oxidative damage of too much oxygen free radicals, especially the damage to cytoplasm membrane. SOD isozyme had been used for characterization of number of plant species. In Pearl millet SOD isozyme had been extensively used for characterization. The present study conducted to characterize 21 genotypes (seven hybrids, six female parents, five male parents and three Open Pollinated Varieties(OPVs) of pearl millet by using SOD isozyme. Five bands were found ranges from Rm values of 0.44 to 0.91. Bands with Rm values of 0.44, 0.56 and 0.89 were monomorphic for all genotypes. Band with Rm value of 0.48 and 0.91 were found to be polymorphic. All genotypes could be grouped into as much as four clusters based on less than 50% Jaccard’s similarity coefficients. Hybrids HHB50 (H1), HHB 67(H3) and HHB 94(H5) were closely resembled (SI=0.8000) with their female parents viz., MS 81A (F1), MS 843A (F3) and ICMA 89111A (F5).


Characterization Cluster Superoxide dismutase.


  1. Almgard, G. and Clapham, D. (1975). Isoenzyme variation distinguishing eighteen Avena cultivars grown in Sweden. Swed. J. Agric. Res., 5: 61–67.
  2. Almgard, G. and Landegren, U. (1974). Isoenzymatic variation used for the identification of barley cultivars. Z. Pflanzenzuecht. 72: 63–73.
  3. Chevreau, E.; Manganaris, A.G. and Gallet, M. (1999). Isozyme segregation in five apple progenies and potential use for map construction. Theor Appl Genet., 98: 329-336.
  4. Chhabra, A.K.; Khairwal, I.S.; Rai, K.N.; Seetharama, N.; Hash C.T. and Gurtu S. (1996). Assessment of degree of isogenicity of tall/dwarf near-isogenic pairs of Pearl millet using isozymes. National Journal of Plant Improvement, 1:41-52.
  5. Draper, S.R. and Craig, E.A. (1981). A phenotypic classification of wheat gliadin electropherograms. J. Natl. Inst. Agric. Bot., 15:390–398.
  6. Gulati, S. (1998). Characterisation of cytoplasmic-nuclear male-sterile line of pearl millet. M.Sc. Thesis, CCS HAU, Hisar.
  7. Hayward, M.D. and McAdam, N.S. (1977) .Isoenzymic polymorphism as a measure of distinctness and stability in cultivars of Lolium perenne. Z. Pflanzenzuecht, 79: 59–68.
  8. Jaccard, P. (1908). Nouvelles recherches sur la distribution forale. Bull. Soc. Vaud Sci Nat., 44: 223-270.
  9. Kumar, M.B.A.; Sherry, R.J.; Varier, A. and Dadlani, M. (2007). Suitability of seed esterases for establishing distinctness, uniformity and stability of pearl millet genotypes. Current Science, 93: 951-956.
  10. Kumar, M.B.A.; Sherry, R.J.; Varier, A. and Sharma, S.P. (2004). Seed esterases – A descriptor for characterization of Pearl millet [Pennisetum glaucum (L.) R.Br.] genotypes. Indian J. Genet., 64: 267–270.
  11. Mancini, R.; Depace, C.; Mugnozza, G.T.S.; Del Re, V. and Vittori, D. (1989). Isozyme gene markers in Vicia faba. Theor. Appl. Genet., 77:657-667.
  12. May, B.; Staub, J.E. and Kuhns, L.J. (1982). Potato cultivars; genetic variation within putative clones. Am. Potato J., 59:179– 187.
  13. PPV&FRA (Protection of Plant Varieties and Farmers’ Rights Act), 2001.
  14. Rao, K.S.S.V.P.; Varier, A.; Mohapatra, T.; Arunakumari, K. and Sharma, S.P. (2001). Electrophoresis of seed esterases and RAPD analysis for identification of hybrids and parental lines of pearl millet [Pennisetum glaucum (L.) R.Br.]. Plant Varieties Seeds, 14: 41–52.Rohlf, P.J. (2000). NTSYSpc. Numerical taxonomy and multivariate analysis system, version 2.01. Applied Biostatistics, New York.
  15. Singh, R. K.; Sharma, R. K.; Singh, A. K.; Singh, V. P.; Singh, N. K.; Tiwari, S. P. and Mohapatra, T. (2004).Suitability of mapped sequence tagged microsatellite site markers for establishing distinctness, uniformity and stability in aromatic rice. Euphytica, 135:135–143.
  16. Suso, M.J. and Moreno, M.T. (1986). Isoenzymatic polymorphism of superoxide dismuatase (SOD) in Vicia faba and its systematic implications. FABIS news letter, 16:3-11
  17. Torres, A.M.; Satovic, Z.; Canovas, J.; Cobos, S. and Cubero, J.I. (1995). Genetic mapping of new isozyme loci in Vicia faba using trisomic. Theor. Appl. Genet., 91:783-789.
  18. Tostain, S.; Riandey, M. F. and Marchais, L. (1987).Enzyme diversity in pearl millet (Pennisetum glaucum). 1. West Africa. Theor. Appl. Genet., 74: 180–193.
  19. UPOV. (1994). Guidelines for the conduct of tests for distinctness, uniformity and stability – barley (Hordeum vulgare), TG/19/10, p. 69.
  20. UPOV. (1996). Guidelines for the conduct of tests for distinctness, uniformity and stability – wheat (Triticum aestivum), TG/3/11, p. 43.
  21. UPOV. (1998). Guidelines for the conduct of tests for distinctness, uniformity and stability – soyabean (Glycine max), TG/80/6, p. 38.
  22. UPOV.(1999). Guidelines for the conduct of tests for distinctness, uniformity and stability – maize (Zea mays), TG/2/6, p. 63.
  23. Varier, A.; Dadlani, M. and Sharma, S.P. (1995). Phenol test and electrophoresis of seed esterases for testing genetic purity in seed lots of pearl millet. Indian J. Agric. Sci., 65: 789–792.
  24. Varier, A.; Vashisht, V. and Agarwal, P.K. (1990). Identification of Pearl millet cultivars using PAGE of soluble proteins and isoenzymes of seed. Int. Conf. Seed Sci.& Tech, New Delhi, 21-25,1990.
  25. Varier, A.; Vashist, V. and Agarwal, P. K. (1993). Identification of composite varieties of pearl millet using polyacrylamide gel electrophoresis of total soluble proteins and isozymes. Seed Res., Spl vol 1: 401–407.
  26. Wendel, J.F. and Weeden, N.F. (1989). Visualisation and interpretation of plant isozymes. In: Isozymes In:Plant Biology, [Soltis D.E. and Soltis P.S. (eds)], Dioscorides Press, Portland, OR, US, pp. 5–45.

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