Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

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  • SJR 0.293

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Indian Journal of Agricultural Research, volume 55 issue 4 (august 2021) : 493-496

Effect of the Different Concentrations of Protective-stimulating Complex (HFC) on the Growth and Development of Grape Seedlings in the Tissue Culture at the Different Nutrient Levels

Esraa M.M. Farahat, S.L. Belopukhov
1Department of Chemistry, Russian State Agrarian University - MTAA named after K.A. Timiryazev, 127550, Timiryazevsky proezd, 2, Moscow, Russian Federation.
Cite article:- Farahat M.M. Esraa, Belopukhov S.L. (2021). Effect of the Different Concentrations of Protective-stimulating Complex (HFC) on the Growth and Development of Grape Seedlings in the Tissue Culture at the Different Nutrient Levels. Indian Journal of Agricultural Research. 55(4): 493-496. doi: 10.18805/IJARe.A-613.
Background: Humic substances plays a vital role in the plant tissue culture as a growth hormone for in vitro propagation of many plant seedlings. The aim of this study was to investigate the effect of added humic-fulvate complex (HFC) at the various concentrations on the growth and development of grape seedlings in in vitro at the different nutrient levels.  
Methods: The cutting of khasansky grape were cultivated on ¼ Murashige and Skoog medium or ½ Murashige and Skoog medium either alone or supplemented with the humic-fulvate complex at the different concentrations at (0.1, 1 and 10 ml/l). Then, they were cultured for 4 weeks under a controlled environment. 
Result: The data observed that the low concentration of Murashige and Skoog medium (¼ MS) for in vitro rooting of grape cv. ‘Khasansky’ either alone or combined with HFC at the various concentrations significantly increased the rooting percentages and the total length of roots and stimulating the rate of vegetative growth compared with cultivated in ½ MS medium either alone or with supplemented with HFC. ¼ MS+ 10 ml/l HFC was the best treatment for improving the growth of khasansky grape seedlings.
  1. Aml, R.M. Yousef, Hala, S. Emam and Saleh, M.M.S. (2011). Olive seedlings growth as affected by humic and amino acids, macro and trace elements applications. Agriculture and Biology Journal of North America. 2(7): 1101-1107. 
  2. Chen, Y. and Aviad, T. (1990). Effect of Humic Substances on Plant Growth. In: Humic Substances in Soil and Crop Sciences: Selected Reading, [MacCarthy, P., C.E. Clapp, R.L. Malcolm and P.R. Bloom (Eds)]. Soil Science Society America, Madison, WI., pp: 161-187.
  3. Dhanapal, S. and Sathish Seka, D. (2013). Humic Acids and its Role in Plant Tissue Culture at Low Nutrient Level. Journal of Academia and Industrial Research. 2 (6): 338-340.
  4. FAO (2018). The Statistical Database (FAOSTAT). Rome, Italy: Food and Agriculture Organization of the United Nations. Available in:
  5. Khan, N., Ahmed, M., Hafiz, I., Abbas, N., Ejaz, S. and Anjum, M. (2015). Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes. OENO One. 49(1): 37-45.
  6. Krithika, V., Ravindra Naik and Pragalyaashree (2015). Functional properties of grape (Vitis vinifera) seed extract and possible extraction techniques - A review. Agricultural Reviews. (36): 313-320.
  7. Kumar Sootahar, M., Zeng, X., Su, S., Wang, Y., Bai, L., Zhang, Y., Li, T. and Zhang, X. (2019). The Effect of Fulvic Acids Derived from Different Materials on Changing Properties of Albic Black Soil in the Northeast Plain of China. Molecules. 24(8): 1535.
  8. Kurmi, U.S., Sharma, D.K., Tripathi, M.K., Tiwari, R., Baghel, B.S. and Tiwari, S. (2011). Plant regeneration of Vitis vinifera (L) via direct and indirect organogenesis from cultured nodal segments. Journal of Agricultural Technology. 7(3): 721-737.
  9. Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
  10. Obsuwan, K., Namchote, S., Sanmanee, N., Panishkan, K. and Dharmvanij, S. (2011). Effect of various concentrations of humic acid on growth and development of eggplant seedlings in tissue cultures at low nutrient level. World Academy of Science, Engineering and Technology. 80: 276-278.
  11. Ramesh Kumar, Afzal Ahmad, R.K. Dular and Devender Chahal (2015). Knowledge and adoption of improved grape cultivation practices in Haryana. India. Agricultural Science Digest. (35): 31-35.
  12. Robert, E.P. (2014). Organic matter, humus, humate, humic acid, fulvic acid and humin: Their importance in soil fertility and plant health. Proceedings of the IEEE Geoscience and Remote Sensing Symposium (IGARSS), pp. 1-5.
  13. Saruhan, V., Kuşvuran, A. and Babat, S. (2011). The effect of different humic acid fertilization on yield and yield components performances of common millet (Panicum miliaceum L.). Scientific Research and Essays. 6: 663-669. 
  14. Singh, I.P. (2002). Micropropagation in citrus - a review. Agricultural Reviews. (23): 1-13.
  15. Перминова, И. В. (2000). Анализ, классификация и прогноз свойств гумусовых кислот: дис. … д-ра хим. наук. М.: МГУ им. М.В. Ломоносова. 359 с. 

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