Indian Journal of Agricultural Research

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Indian Journal of Agricultural Research, volume 56 issue 4 (august 2022) : 422-428

AMMI Analysis of Yield Performance in Foxtail Millet [Setaria italica (L.) P. Beauv.] Genotypes for Adaptation to Rainfed Conditions in Andhra Pradesh

L. Madhavilatha1,*, C.V. Chandra Mohan Reddy1, M. Shanthi Priya1, N. Anuradha1, R. Narasimhulu1, Y. Pushpa Reni1, C. Kiran Kumar Reddy1, M. Hemanth Kumar1
1Department of Genetics and Plant Breeding, Agricultural Research Station, Tirupati-517 505, Andhra Pradesh, India.
Cite article:- Madhavilatha L., Reddy Mohan Chandra C.V., Priya Shanthi M., Anuradha N., Narasimhulu R., Reni Pushpa Y., Reddy Kumar Kiran C., Kumar Hemanth M. (2022). AMMI Analysis of Yield Performance in Foxtail Millet [Setaria italica (L.) P. Beauv.] Genotypes for Adaptation to Rainfed Conditions in Andhra Pradesh . Indian Journal of Agricultural Research. 56(4): 422-428. doi: 10.18805/IJARe.A-5950.
Background: Foxtail millet is a short duration crop which is suitable for different cropping systems in rainfed farming. Improvement of yields is necessary to achieve profits in rainfed situation which is highly influenced by the high yielding varieties. Genotype by environment interaction (G×E) causes genotypes failure to keep high performance in all environments. Location specific climatic conditions also influence significantly genotype × environment (G×E) interaction, so with the result that identifying stable genotypes for rainfed situations is difficult. The study was conducted with a prime objective to identify stable high yielding foxtail millet genotypes for rainfed cultivation and to identify ideal mega-environments using additive main effects and multiplicative interaction stability model analysis. 

Methods: Four prerelease promising foxtail millet genotypes including three released popular check varieties were evaluated across six locations in Andhra Pradesh during Kharif, 2018 under rainfed situation. 

Result: The culture, SiA 3159 was found to be stable performer across the locations indicating that it is suitable for wide range of environments. In contrast the genotypes SiA 3085 and SiA 3156 showed narrow adaptation, specifically adapted to Anantapur (rainfed situation having scarce rainfall) and Vizianagaram (favorable environments with assured rainfall areas) respectively.
Foxtail millet [Setaria italica (L.) P. Beauv.] is one of the world’s oldest cultivated crop and ranks second in the world’s total production of millets. It is an important staple food for millions of people in southern Europe and Asia (Marathee, 1993). In India the crop area has declined and cultivated in a limited area of around 0.1 million hectares in sporadic patches in the states of Andhra Pradesh, Karnataka, Tamil Nadu, Maharashtra, Madhya Pradesh, Uttar Pradesh and North Eastern states with an annual production of 0.29 million tones and productivity of 600 kg /ha (Anand et al., 2020). Millets are known to possess unique features of resilience to adverse environments, especially during drought and infertile soil conditions (Nadeem et al., 2020). Foxtail millet is a short duration crop which is suitable for different cropping systems in rainfed farming. Foxtail millet grains are rich in starch, proteins and lipids as well as vitamins and minerals, which makes it a good source of nutrients in the human diet in many parts of the world, especially in Africa, China and India where food security has always been the primary concern (Bai et al., 2008). Improvement of yields is necessary to achieve profits in rainfed situation which is highly influenced by the high yielding varieties. Development of high yielding varieties has always been the ultimate objective of crop breeders especially those who do research on orphan crops. Although research on the development of high yielding varieties have led to release of a large number of new varieties in different crops, genotype by environment interaction (G×E) causes genotypes failure to keep high performance in all environments (Mohammadi and Nader, 2008). So, yield stability has been considered as important as the yield potential in plant breeding and will be of particular concern by continuing changes in climatic condition (Fasahat et al., 2015). The newly developed genotypes which perform best in advanced yield trials are promoted to MLTs to assess the stable performance and adaptability across different environments. The genotype environment interaction results in complex data which needs an effective statistical analysis tool for better interpretation. Location specific climatic conditions also influence significantly to genotype× environmental (G×E) interaction, so with the result that identifying stable genotypes for rainfed situations is difficult. Among all the statistical models propounded for dealing with G×E data, the addictive main effects and multiplicative interactions (AMMI) Model (Zobel et al., 1988) is the best to analyze the stable performance of the entries.
       
The best practice of AMMI involves (1) Analysis of variance (ANOVA) (2) Model diagnosis (3) Mega-environment delineation (4) agricultural recommendations to exploit both broad and narrow adaptation to increase yields (Gauch, 2013). Therefore in the present study, AMMI stability analysis was utilized for the data collected from Multilocation trial conducted in six locations under rainfed condition during Kharif, 2018. The main objective of the study was to identify high yielding stable foxtail millet genotypes for general and specific adaptation to rainfed conditions and to investigate the mega environment specific genotypes which can be suggested for cultivation under rainfed situations.
The study was conducted to evaluate seven foxtail millet genotypes developed from Regional Agricultural Research Station, Nandyal in multi-location trial in six locations across Andhra Pradesh in rainfed situation during Kharif, 2018. Description of these six locations is given in Table 1. Four promising prerelease foxtail millet cultures (SiA 3159, SiA 4148, SiA 4201 and SiA 4203) were evaluated along with three checks (Suryanandi, SiA 3156 and SiA 3085). Details of these genotypes along with pedigree are given in Table 2. In six locations, experiment was raised during Kharif, 2018 in randomized complete block design (RCBD) with three replications. Each plot consisted of ten rows of 3 m length with a spacing of 22.5 cm×7-10 cm. Recommended package of practices were followed to raise the crop under rainfed situation. Observations were recorded for plant height, number of productive tillers per plant, panicle length and grain yield.
 

Table 1: Description of locations used for the evaluation of foxtail millet genotypes.


 

Table 2: List of foxtail millet genotypes and their parentage used in the study.


       
AMMI Model was used to analyze the G×E interactions. The procedures of Ebdon and Gauch (2002 a, b) and Gauch (2013) were used for AMMI Model analysis and accuracy gain. The AMMI Model applies ANOVA to partition the variation into the main effects viz., genotype (g in AMMI), environment (e in AMMI) and GEI and then it applies Principal Components Analysis (PCI) to the data. According to Gauch (2013), model diagnosis is useful to determine the best AMMI model family for a given data set and is advised to use FR- test (Cornelius, 1993) to assess model diagnosis and to identify significant interaction principal components (IPCs) in the AMMI model using AMMISOFT software for the analysis of the data. AMMI constitutes a model family with AMMI 0 having no IPC, AMMII 1 having 1 IPC, AMMI 2 having 2 IPC and so on up to AMMIF (residual discarded).
       
The ratio of yield for AMMI winners within each environment (identified in the first column of AMMI ranks) was calculated by dividing the yield for the overall winner (Gauch, 2013). According to Gauch (2013) a ratio of 1 represents a winning genotype across environments. This ratio is an assessment of the importance of narrow adaptation due to GEI effects, with a ratio of ≥1.10 indicative of narrow adaptation.
ANOVA and identification of AMMI model families
 
Analysis of variance for grain yield using AMMI model is presented in Table 3. The main effects genotype (G), environmental (E) and their interaction (GEI) components were statistically significant at p≤0.001. The environmental component showed the largest proportion of variation 62.38% followed by G´E interaction components (25.02%) and least variation by genotypic component (4.42%). The large variation for environments indicated that the environments were diverse with large differences among environmental means causing most of the variation in grain yield which is in harmony with the findings of Molla et al., (2013). The variation due to GEI was higher than genotype variation indicating that there were substantial genotypic responses across environments. Presence of significant GEI was also reported earlier by Misra et al., (2009). The total variation of GEI consists of GEIN and GEIS with GEIN estimated simply by multiplying the error mean square by the number of degrees of freedom of GEI and GEIS obtained by subtracting GEIN from GEI (Gauch, 2013). The GEI effects were partitioned into four IPCs (IPC1, IPC2, IPC3 and IPC4). IPC1, IPC2 and IPC3 were found to the significant at P≤0.001 for grain yield in different environments under rainfed conditions. In terms of contribution to the total GEI for grain yield IPC1 alone contributed to 59.07%, (IPC1 and IPC2 cumulatively contributed 84.84%) and IPC1 to IPC3 contributed 94.94%. Based on statistical and practical considerations model evaluation is essential to determine the best AMMI Model family for grain yield. Three AMMI model families were identified based on the FR-test at P≤0.01 for grain yield in the different environments (Table 3). The AMMI model captured 88.33% of the GEIs (GEI Signal) and 11.67 of the GEIN (GEI Noise). Sum of squares for GEIS and GEIN was 5 fold and 0.66 fold respectively that of genotype main effect. The results show that the AMMI model as used in this study was appropriate and worthwhile, since the SS for GEIS and also SS for GEI are not buries in GEIN. AMMI is not a single model; rather it constitutes a model family, AMMI0 to AMMIF. AMMI0 captures no GEIN and GEIS whereas AMMIF, the full model equals the actual data so it has no residual and captures all GEIN and GEIS. Therefore, model selection is one of the most important steps in AMMI analysis. Model diagnosis provides cues for selecting the best model for a given dataset (Gauch, 2013). The results clearly indicate that IPC1, IPC2 and IPC3 represent the AMMI model families AMMI 1, AMMI 2 and AMMI 3 respectively, cumulatively covering 94.94% of the GEI variation and 107.47% of the GEIs variation. This indicates the AMMI biplot model is the best fit for the data set which is in agreement with Naveed et al., (2007). In AMMI biplot 1 IPC1 scores of genotypes and environments are plotted against their respective means, the plot is helpful in visualizing the average productivity of the genotypes, environments and their interaction for all possible genotype-environment combinations (Fig 1). Genotypes that group together have similar adaptation while environments which group together influence the genotypes in the same way. AMMI2 model family delineated three mega-environments with three winner genotypes namely SiA 3159, SiA 3085 and SiA 4203 (Table 4). AMMI biplot 2 shows the GE interaction patterns beyond those captured in an ordinary AMMI1 biplot, helps in visual interpretation of the GEI patterns and identification of the genotypes or locations that exhibits a low, medium or high level of interaction effects (Fig 2). Genotypes near the origin are non-sensitive to environmental interactive forces and those distant from the origin are sensitive to environment and have large interactions. The points of either genotypes or environments that are close to each other have similar interaction patterns, while those distant from each other have different interaction patterns (Krishnamurthy et al., 2021 and Simon et al., 2018).
 

Table 3: Analysis of variance for grain yield in foxtail millet genotypes in rainfed conditions across six locations during 2018.


 

Fig 1: Scattered distribution patterns of 7 foxtail millet genotypes and 6 environments presented in the AMMI model biplot 1 with grain mean yield (q/ha).


 

Table 4: “Winner” genotypes and numbers of mega-environments for the additive main effects and multiplicative interaction (AMMI) model family for foxtail millet genotypes evaluated in six environments in rainfed conditions.


 

Fig 2: Scattered distribution patterns of 7 foxtail millet genotypes and 6 environments presented in AMMI biplot 2 showing IPC1 and IPC2 scores.


 
Identification of winner genotypes from the AMMI model family
 
A mega-environment is defined as a group of locations that consistently shares the best set of genotypes or cultivars across years (Yan and Rajcan, 2002). Mega-environments are distinguished by having different genotype winners. Increasingly complex AMMI models generally have more genotype winners. Winner genotypes identified using the AMMI model family for yield traits are shown in Table 4. Genotypes were listed based on IPC1 scores with the top and bottom order genotypes have contrasting GEI patterns. The AMMI constituted a model family from AMMI 0 to AMMI F with AMMI 0 having one winner genotype in one mega environment whereas the AMMI F consisted five winner genotypes with five mega environments. The genotype SiA 3159 won in all AMMI model families and it also won interms of maximum number of environments with 6,4,3,2,3 and 2 in the AMMI 0, AMMI 1, AMMI 12, AMMI 3, AMMI 4 and AMMI F model families respectively. According to the standard AMMI diagnosis model, an intermediate AMMI model such as AMMI I or AMMI 2 is predicatively accurate. In the present study AMMI1 and AMMI2 delineated three and three mega environments based on IPC1 and IPC2 scores respectively with each environment having one genotype. According to AMMI 1 in addition to genotype SiA 3159 the genotype SiA 3085 and SiA 3156 also won in one and one mega environments respectively. Similar results were obtained by Wedajo et al., (2018) and Krishnamurthy et al., (2017). In the present experiment the AMMI3 model family had the maximum predictive accuracy representing 4 environments with 4 winning genotypes. According to the AMMI 3 model family genotypes SiA 3159, SiA 4201, SiA 4203 and SiA 3085 won in 2, 2, 1 and 2 different mega environments respectively. Among the four genotypes of the AMMI3 model family the genotype SiA 3159 had maximum grain yield of 26.04 q/ha which was more than overall mean yield of 22.19 q/ha under different environments in rainfed conditions. Therefore SiA 3159 is the winning genotype with a ratio of 1.0 is broadly adopted for different environments. In contrast the genotype SiA 3085 and SiA 3156 showed narrow adaptation, specifically adapted to Anantapur (rainfed situation having scarce rainfall) and Vizianagaram (favorable environments with assured rainfall areas) respectively.
   
Delineation of mega environments based on AMMI
 
The ranking of the five top performing foxtail millet genotypes across the test environments based on AMMI 1 and AMMI F ranking is presented in Table 5. The environments in the table are listed based on the IPC1 scores with the top and bottom ordered environments have contrasting GEI patterns. According to AMMI1 model 6 environments were delineated into 3 mega environments. The first mega environment was the largest and consisted of 4 different environments (Fig 3). The second mega environment consisted of single environment namely ANT 3 as did the third mega environment VIN 5. AMMIF delineated five mega environments with five genotypes. Adaptive responses for foxtail millet genotypes according to the AMMI model are shown in Fig 4. According to the AMMI1 model genotype SiA 3159 was the winner genotype in four environments of mega environment 1 (Fig 4). This is an ideal genotype in terms of high performance and stability over rainfed areas in comparison to the other genotypes. Similar results were observed in studies by Bose et al., (2014) and Reza and Mohammad (2020). The genotype SiA 3159 may be given as minikits for testing in farmers’ fields in different zones of Andhra Pradesh. Recently released promising varieties SiA 3085 and SiA 3156 were included as checks in the study were ranked second and won in one environment each.
 

Table 5: Ranking table showing the top 5 foxtail millet genotypes according to AMMI I and AMMIF model families.


 

Fig 3: AMMI mega-environment display for 7 foxtail millet genotypes evaluated under 6 environments.


 

Fig 4: Adaptive responses for foxtail millet genotypes according to the AMMI1 model.

Foxtail millet is a minor millet crop in India cultivated in limited areas having strong adaptability to rainfed areas with minimum yields. In the recent past the area under this crop is increasing which necessitates to develop new high yielding varieties. Under rainfed situations, for making this crop as a remunerative, the varieties should have better adaptability for various environments. Experimental results revealed significant differences among genotype (G), environment (E) and their interaction (GEI) components. SiA 3159 was emerged as winner genotype in four environments of mega environment 1. This is an ideal genotype in terms of high performance and stability over rainfed areas in comparison to the other genotypes. The genotype SiA 3159 suggested for testing minikits in farmers’ fields in different zones of Andhra Pradesh.
None.

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