Indian Journal of Agricultural Research

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Indian Journal of Agricultural Research, volume 56 issue 4 (august 2022) : 375-380

Identification of Parental Lines and Hybrid using Molecular Techniques in CGMS based Chilli (Capsicum annuum L.) Hybrid UARChH42 (JCH42)

Neha Thakur, S.N. Vasudevan, B.V. Tembhurne, S.R. Doddagoudar
1College of Horticulture and Forestry, Thunag-175 048, Mandi, Himachal Pradesh, India.
Cite article:- Thakur Neha, Vasudevan S.N., Tembhurne B.V., Doddagoudar S.R. (2022). Identification of Parental Lines and Hybrid using Molecular Techniques in CGMS based Chilli (Capsicum annuum L.) Hybrid UARChH42 (JCH42). Indian Journal of Agricultural Research. 56(4): 375-380. doi: 10.18805/IJARe.A-5893.

ABSTRACT

Background: Molecular markers are the landmarks on DNA that identifies a particular sequence of base pairs coding for a character. SCAR (Sequenced Characterized Amplified Region) markers are proving to be more effective in identification of genotypes as they are PCR based co-dominant markers. In view of plant variety registration under PPV and FRA, 2001 molecular characterization/ identification of the variety is essential to ascertain the trueness of the variety, hence present studies have been planned and executed.
Methods: In present investigation CGMS based chilli hybrid UARChH42 and its parental lines were identified using molecular techniques. A line, B line, R line and hybrid seedlings were used for DNA extraction and characterized on the basis of polymorphism with respect to sterility or fertility by using SCAR markers.
Result: P1 and P2 and coxII-SCAR which are sterility specific markers could amplify A line and hybrid showing a definite band. CRF-SCAR 870 is a fertility specific marker amplified R line and hybrid. Also CMS-SCAR 130 and CMS-SCAR 130/140 were able to identified A line, B line, R line and hybrid. Two of the markers viz. orf-456-SCAR atp6-SCAR and were not able to specify any case of parental lines or hybrid identification. Hybridity of UARChH42 (JCH42) chilli hybrid was determined by any similarity in the banding pattern with any of its parent. This study would help in the fulfillment of the requirements of protection of plant varieties and farmers’ rights authority (PPVFRA), New Delhi for registration purpose.

REFERENCES


  1. Anonymous, (2019). Selected State-wise Area, Production and Productivity of Dry Chillies in India (2015-16 and 2016-17). https://www.indiastat.com

  2. Aulakh, P.S. (2016). Molecular mapping of nuclear male sterility gene ms10 in chilli pepper (Capsicum annuum L.) Ph.D. Thesis. Punjab Agricultural University, Ludhiana. 

  3. Budar, F., Touzet, P. and De, P.R. (2003). The nucleo-mitochondrial conflict in cytoplasmic male sterilities revisited. Genetica. 117: 3-16.

  4. Chen, F., Zhu, S.W., Xiang, Y., Jiang, H.Y. (2010). Molecular marker- assisted selection of the ae alleles in maize. Genetics and Mol. Res. 9: 1074-1084.

  5. Dhaliwal, M.S. and Jindal, S.K. (2014). Induction and exploitation of nuclear and cytoplasmic male sterility in pepper (Capsicum spp.): A review. J. Hort. Sci. and Biotech. 89(5): 471-79.

  6. Lin, S.W., Shieh, H.C., Wang, Y.W., Tan, C.W., Schafleitner, R., Yang, W.J. and Kumar, S. (2015). Restorer breeding in sweet pepper: Introgressing Rf allele from hot pepper through marker-assisted backcrossing. Scientia Horticulturae. 197: 170-75.

  7. Mokate, A.S. (2009). Genetic analysis of CGMS based hybrids using newly identified fertility restorer lines and confirmation through molecular markers in chilli (Capsicum annuum L.). Thesis. Anand Agricultural University, Gujarat, India.

  8. Oland, M.L. and Porter, A.M. (1941). A study of natural crossing in peppers, Capsicum frutesences. Proc. Am. Soc. Horticulture Sci. 38: 585-588.

  9. Paran, I. and Michelmore, R.W. (1993). Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theoretical and Appl. Genetics. 85(8): 985-93.

  10. Schnable, P.S. and Wise, R.P. (1998). The molecular basis of cytoplasmic male sterility and fertility restoration. Trends in Plant Sci. 3: 175-180.

  11. Sun, G.S., Dai, Z.L., Bosland, P.W., Wang, Q., Sun, C.Q., Zhang, Z.C. and Ma, Z.H. (2017). Characterizing and marker-assisting a novel chili pepper (Capsicum annuum L.) yellow bud mutant with cytoplasmic male sterility. Genetic and Mol. Res. 16(1): 1-11.

  12. Tembhurne, B.V., Babu, K., Gurumurthi, N.K., Biradar, V., Biradar, B. and Lokesha, R. (2016). Molecular characterization of CMS lines and standardization of hybrid seed production technique in chilli (Capsicum annuum L.). J. Agricultural Sci. and Technol. 6: 238-243.

  13. Yeh, T., Lin, S., Shieh, H., Teoh, Y. and Kumar, S. (2016). Markers for cytoplasmic male sterility (CMS) traits in chilli peppers (Capsicum annuum L.). I: multiplex PCR and validation, SABRAO. J. Breed. and Genetics. 48(4): 465-73.
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