Indian Journal Of Agricultural Research

Study on Antifungal Activity and Ability Against Rice Leaf Blast Disease of Nano Cu-Cu2O/Alginate

DOI: 10.18805/IJARe.A-582    | Article Id: A-582 | Page : 802-806
Citation :- Study on Antifungal Activity and Ability Against Rice Leaf Blast Disease of Nano Cu-Cu2O/Alginate.Indian Journal Of Agricultural Research.2020.(54):802-806
Doan Thi Bich Ngoc, Bui Duy Du, Le Nghiem Anh Tuan,Bui Dinh Thach, Chu Trung Kien, Dang Van Phu, Nguyen Quoc Hien hien7240238@yahoo.com
Address : Research and Development Center for Radiation Technology, Vietnam Atomic Energy Institute, Ho Chi Minh City 700000, Vietnam.
Submitted Date : 7-07-2020
Accepted Date : 7-11-2020
First Online:

Abstract

Background: Rice blast disease due to Pyricularia oryzae fungus is one of the most destructive ones for rice, causing serious losses in yield and quality in Vietnam and other countries. Studying to develop new fungicides to control the fungal disease effectively is essential.
Methods: The sodium alginate-stabilized Cu-Cu2O nanocolloidal solution with a size of about 4 nm and the Cu concentration of 5,000 mg/L was prepared by chemical method. The antifungal activity in vitro against P. oryzae and the ability to control the rice blast disease in the greenhouse of nano Cu-Cu2O/alginate were assessed.
Result: The nano Cu-Cu2O/alginate exhibited highly fungal activity with the IC50 of 17.8 mg Cu /L. The disease severity of nano Cu-Cu2O/alginate treatments was in the range of 9.38 - 18.54% in comparison with 47.57% of the untreated control. The grain yield of rice plants treated with nano Cu-Cu2O/alginate increased compared with that of the untreated control, and the Cu content in polished rice was almost the same as that of usually cultured polished rice. Thus, the nano Cu-Cu2O/alginate can be used in agriculture as a plant fungicide, especially for rice cultivation.

Keywords

Alginate Antifungal activity Nano Cu-Cu2O Pyricularia oryzae Rice blast disease

References

  1. Anwar, A. and Bhat, M.S. (2005). Efficacy of fungicides as seed treatment in the management of blast disease of rice in nursery bed. Agricultural Science Digest. 25(4): 293-295.
  2. Begum, M., Bordoloi, B.C., Singha, D.D. and Ojha, N.J. (2018). Role of seaweed extract on growth, yield and quality of some agricultural crops: A review. Agricultural Reviews.    39(4): 321-326.
  3. Chen, W-C., Chiou, T-Y., Delgado, A.L. and Liao, C-S. (2019). The control of rice blast disease by the novel biofungicide formulations. Sustainability. 11(12): 3449-3457. 
  4. Du, B.D., Ngoc, D.T.B., Thang, N.D., Tuan, L.N.A., Thach, B.D. and Hien, N.Q. (2019). Synthesis and in vitro antifungal efficiency of alginate-stabilized Cu2O-Cu nanoparticles against Neoscytalidium dimidiatum causing brown spot disease on dragon fruit plants (Hylocereus undatus). Vietnam Journal of Chemistry. 57(3): 318-323.
  5. Elamawi, R.M.A. and El-Shafey, R.A.S. (2013). Inhibition effects of silver nanoparticles against rice blast disease caused by magnaporthe grisea. Egyptian Journal of Agricultural Research. 91(4): 1271-1281.
  6. Giannousi, K., Sarafidis, G., Mourdikoudis, S., Pantazaki, A. and Dendrinou-Samara, C. (2014). Selective synthesis of Cu2O and Cu/Cu2O NPs: antifungal activity to yeast saccharomyces cerevisiae and DNA interaction. Inorganic Chemistry. 53(18): 9657-9666. 
  7. Hans, M., Erbe, A., Mathews, S., Chen, Y., Solioz, M. and Müclich, F. (2013). Role of copper oxides in contact killing of bacteria. Langmuir. 29(52): 16160-16166.
  8. Huang, S., Wang, L., Liu, L., Hou, Y. and Li, L. (2015). Nanotechnology in agriculture, livestock. and aquaculture in China. A review. Agronomy for Sustainable Development. 35(2): 369-400.
  9. Kanhed, P., Birla, S., Gaikwad, S., Gade, A., Seabra, A.B., Rubilar, O., Duran N. and Rai, M. (2014). In vitro antifungal efficacy of copper nanoparticles against selected crop pathogenic fungi. Materials Letters. 115: 13-17.
  10. Kanmani, N. (2018). Biosynthesis of silver nanoparticles and their evaluation of antifungal activity against Magnaporthe oryzae. Journal of Pharmacognosy and Phytochemistry. 7(3): 1638-1648.
  11. Kavitha, M.P., Ganesaraja, V. and Paulpandi, V.K. (2008). Effect of foilar spraying of sea weed extract on growth and yield of rice (Oryza satival L.). Agricultural Science Digest. 28(2): 127-129.
  12. Koutroubas, S.D., Katsantonis, D., Ntanos, D.A. and Lupotto, E. (2009). Blast disease influence on agronomic and quality traits of rice varieties under Mediterranean conditions. Turkish Journal of Agriculture and Forestry. 33: 487-494.
  13. Kulmitra, A.K., Sahu, N., Kumar, V.B.S., Thejesha, A.G., Ghosh, A. and Gulnaz, Y. (2017). In vitro evaluation of bio-agents against Pyricularia oryzae (Cav.) causing rice blast disease. Agricultural Science Digest. 37(3): 247-248.
  14. Rusjan, D. (2012). Copper in horticulture, In “Fungicides for Plant and Animal Diseases” (Eds. by Dhanasekaran, D., Thajuddin, N. and Panneerselvam, A.), published by Intech, Rijecka, Croatia. 257-278.
  15. Punshon, T. and Jackson, B.P. (2018). Essential micronutrient and toxic trace element concentrations in gluten containing and gluten-free foods. Food Chemistry. 252: 258-264.
  16. Vi, N.T.T. and Liu, T. (2019). Improve the competitiveness and value of rice exports of the Mekong Delta provinces. Agricultural Sciences. 10(6): 707-719.
  17. Xin, W., Mao, Y., Lu, F., Li, T., Wang, J., Duan, Y. and Zhou, M. (2020). In vitro fungicidal activity and in planta control efficacy of coumoxystrobin against Magnaporthe oryzae. Pesticide Biochemistry and Physiology. 162: 78-85.
  18. Xu, J., Yang, L., Wang, Z., Dong, G., Huang, J. and Wang, Y. (2006). Toxicity of copper on rice growth and accumulation of copper in rice grain in copper contaminated soil. Chemosphere. 62(4): 602-607.

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