Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

  • NAAS Rating 5.20

  • SJR 0.293

Frequency :
Bi-monthly (February, April, June, August, October and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Agricultural Research, volume 56 issue 5 (october 2022) : 599-606

Chitosan as a Growth Promoter and Enhance Survival Rate in an in vitro Culture of Banana (Musa spp.) Cultivar ‘Bantala’

Laxman Kandha, Rahul Kumar, Birendra Kumar Bindhani
1Plant Tissue Culture Lab, School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar-751 024, Odisha, India.
Cite article:- Kandha Laxman, Kumar Rahul, Bindhani Kumar Birendra (2022). Chitosan as a Growth Promoter and Enhance Survival Rate in an in vitro Culture of Banana (Musa spp.) Cultivar ‘Bantala’. Indian Journal of Agricultural Research. 56(5): 599-606. doi: 10.18805/IJARe.A-5747.
Background: Banana (Musa spp.) is one of the most consumable fruits and cultivated around the globe. It contains high nutritional value as well as the high demand of the market. The microbes are the main problem for the propagation of banana plants in tissue culture. Chitosan is one of the best substances for the eradication of contamination and also growth stimulators of banana plants. This study is based on the micro-propagation of the bantala variety of Musa species and free from microbe infection. 
Methods: The rhizome and sucker as explants of Musa cv. Bantala. The different combination concentrations of 6-Benzylaminopurine (BAP), indole-3-acetic acid (IAA) and chitosan (CS) were tried in Murashige and Skoog medium for in vitro response of plants, shoot initiation and shoot proliferation. The formation of rooting was used as the half-strength Murashige and Skoog (MS) medium with indole-3-butyric acid (IBA) and chitosan (CS).
Result: The best response, shoot initiation and shoot proliferation were observed at 6-Benzylaminopurine (5.0 mg/L)+indole-3-acetic acid (0.5 mg/L)+chitosan (25 mg/L) and 6-Benzylaminopurine (4.0 mg/L)+indole-3-acetic acid (0.5 mg/L)+chatoyant (25 mg/L) in both of rhizome and sucker respectively. The maximum root formations were observed in the medium containing half-strength Murashige and Skoog medium+1.0 mg/L indole-3-butyric acid+25 mg/L chitosan in the rhizome and 0.8 mg/L indole-3-butyric acid+25 mg/L chitosan in the sucker. The successful survival rate of sucker and rhizome under the acclimatization condition was recorded as 90% and 88% compared with control as 66% and 63% respectively. This standardized protocol might be useful for the mass production of bantala variety as well as other cultivars of banana plants.

  1. Ahmed, S., Sharma, A., Singh, A.K., Wali, V.K. and Kumari, P. (2014). In vitro multiplication banana (Musa sp.) cv. Graindnaine. African Journal of Biotechnology. 13: 2696-2703.

  2. AitBarka, E.A., Eullaffroy, P., Clement, C. and Vernet, G. (2004). Chitosan improves development and protects Vitis vinifera L. against Botrytis cinerea. Plant Cell Report. 22: 608-614.

  3. Benhamou, N., Lafontaine, P.J. and Nicole, M. (1994). Induction of systemic resistance to Fusarium crown and root rot in tomato plants by seed treatment with chitosan. Phytopathology. 84: 1432-1444.

  4. Borah, A.R., Anbumalarmathi, J. and Sharmili, S.A. (2019). In vitro propagation of [Coccinia indica (L.) Voigt]. from internodal segments. Indian Journal of Agricultural Research. 53: 202-207.

  5. Cassells, A.C., Kowalski, B., Fitzgerald, D.M. and Murphy, G.A. (1999). The use of image analysis to study developmental variation in micropropagated potato (Solanum tuberosum L.) plants. Potato Research. 42: 541-548. 

  6. Chaurasia, A.K., Patil, H.B., Krishna, B., Subramaniam, V.R., Sane, P.V. and Sane, A.P. (2017). Flowering time in banana (Musa spp.), a day neutral plant, is controlled by at least three Flowering Locus T homologues. Scientific reports. 7: 5935. 

  7. Darvari, F.M., Sariah, M., Puad, M.P. and Maziah, M. (2010). Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers. African Journal of Biotechnology. 9: 2360-2366. 

  8. Deo, B. and Pradhan, B. (2017). Effects of plant growth hormones on shoot proliferation of Musa paradisiaca cv. Bantal. International Journal of Plant Sciences. 12: 135-138.

  9. El-Ghaouth, A., Arul, J., Grenier, J., Benhamou, N., Asselin, A. and Belanger, R. (1994). Effect of chitosan on cucumber plants: Suppression of Pythium aphanidermatum and induction of defense reactions. Phytopathology. 84: 313-320. 

  10. Habiba, U., Reza, S., Saha, M.L., Khan, M.R. and Hadiuzzaman, S. (2002). Endogenous bacterial contamination during in vitro culture of table banana: Identification and prevention. Plant Tissue Culture. 12: 117-124.

  11. Hussein, N. (2012). Effects of nutrient media constituents on growth and development of banana (Musa spp.) shoot tips cultured in vitro. African Journal of Biotechnology. 11(37): 9001-9006.

  12. Kaçar, Y.A. and Faber, B. (2012). Micropropagation of banana. Plant Cell Culture Protocols. pp. 143-151. 

  13. Muhammad, A., Rashid, H. and Hussain, I. (2007). Proliferation rate effects of BAP and kinetin on banana (Musa spp. Aaa group) ‘Basrai’. Horticulture Science. 42: 1253-1255.

  14. Nge, K. L., Nwe, N., Chandrkrachang, S. and Stevens, W.F. (2006): Chitosan as a growth stimulator in orchid tissue culture. Plant Science. 170: 1185-1190.

  15. Ohta, K., Tanguchi, A., Konishi, N. and Hosoki, T. (1999). Chitosan treatment affects plant growth and flower quality in Eustomagrandiflorum. Hortscience. 34: 233-234.

  16. Ortiz, R. and Vuylsteke, D. (1996). Recent advances in Musa genetics, breeding and biotechnology. Plant Breeding Abstract. 66: 1355-1363.

  17. Pérez-Hernández, J.B. and Rosell-García, P. (2008). Inflorescence proliferation for somatic embryogenesis induction and suspension-derived plant regeneration from banana (Musa AAA, cv.‘Dwarf Cavendish’) male flowers. Plant Cell Reports. 27: 965-971. 

  18. Pradeep, K.P., Zachariah, G., Estellita, S. and Suma, A. (1992). Field performance of banana tissue culture plants of variety Nendran (Musa AAB). South Indian Horticulture. 40: 4.

  19. Rabea, E.I., Badawy, M.E.T., Stevens, C.V., Smagghe, G. and Steurbaut, W. (2003). Chitosan as antimicrobial agent: applications and mode of action. Biomacromolecules. 4: 1457-1465.

  20. Rathod, S. and Mishra, G.C. (2018). Statistical models for forecasting mango and banana yield of Karnataka, India. Journal of Agricultural Science and Technology. 20: 803-816.

  21. Roby, D., Gadelle, A. and Toppan, A. (1987). Chitin oligosaccharides as elicitors of chitinase activity in melon plants. Biochemical and Biophysical Research Communications. 143: 885-892. 

  22. Rout, G.R., Samantaray, S. and Das, P. (2000). Biotechnology of the banana: A review of recent progress. Plant biology. 2: 512-524.

  23. Saravanapandeeswari, V. and Vanitha, B. (2018). Growth of area, production and productivity of banana (Musa paradisiaca) cultivation in Theni district, Tamil Nadu-An analysis by component elements. Indian Journal of Agricultural Research. 52: 107-110.

  24. Sarma, I., Deka, A.C. and Sarma, T.C. (2021). A protocol for rapid clonal propagation and microrhizome production of Curcuma caesia Roxb. (Zingiberaceae): A critically endangerd medicinal plant of North East India. Indian Journal of Agricultural Research. 55(1): 13-22.

  25. Sidha, M., Suprasanna, P., Bapat, V.A., Kulkarni, U.G., andShinde, B.N. (2007). Developing somatic embryogenic culture system and plant regeneration in banana. Bhabha Atomic Resource Centre (BARC) Newslett. 285: 153-161. 

  26. Sopalun, K., Thammasiri, K. and Ishikawa, K. (2010). Micropropagation of the thai orchid Grammatophyllum speciosum blume.Plant Cell, Tissue and Organ Culture. 101: 143-150.

  27. Sultan, M.T., Khan, M.H., Hakim, M.L., Mamun, A.N.K., Morshed, M.A., Islam, M.R., and Islam, M.R. (2011). In vitro plant regeneration from male flowers of banana. International Journal of Biosciences. 1: 1-11. 

  28. Tiuterev, S., Yakubchik, M., Tarlakovsky, S., Popova, E., Vytsky, V. and Dorofeyeva, T. (1996). Chitosan: mechanism of action and ways of using as ecologically safe means in enhancement of plant disease resistance. Archives of Phytopathology and Plant Protection. 30: 323-332. 

  29. Uthairatanakij, A., Teixeira da Silva, J.A. and Obsuwan, K. (2007). Chitosan for improving orchid production and quality.Orchid Science and Biotechnology. 1: 1-5.

  30. Vander, P., Vårum, K.M., Domard, A., El Gueddari, N.E. and Moerschbacher, B.M. (1998). Comparison of the ability of partially N-acetylated chitosans and chitooligosaccharides to elicit resistance reactions in wheat leaves. Plant Physiology. 118: 1353-1359. 

  31. Walker-Simmons, M., Hadwiger, L. and Ryan, C.A. (1983). Chitosans and pectic polysaccharides both induce the accumulation of the antifungal phytoalexinpisatin in pea pods and antinutrient proteinase inhibitors in tomato leaves. Biochemical and biophysical research communications. 110: 194-199. 

  32. Wambugu, F.M., Njuguna, M.M., Acharya, S.S. and Mackey, M.A. (2008). Socio-economic impact of tissue culture banana (Musa spp.) in Kenya through the whole value chain approach. In IV International Symposium on Banana: International Conference on Banana and Plantain in Africa: Harnessing International. 879: 77-86.

Editorial Board

View all (0)