Chief EditorT. Mohapatra
Print ISSN 0367-8245
Online ISSN 0976-058X
NAAS Rating 5.20
Antioxidant Responses of Arsenite-induced Oxidative Stress in Rice (Oryza sativa L.) and its Modulation by Eugenol (Extracted from Ocimum sanctum)
First Online 19-10-2021|
Methods: In the experiment 7 day old rice seedlings (IR-64) were exposed to 10,50,100 µM of arsenite separately and co-treatment with 10,50,100 µM eugenol in a hydroponic medium for 7 days. The activity of antioxidant enzymes such as superoxide dismutase, ascorbate peroxidase, glutathione peroxidase, catalase and lipid peroxidation (malondialdehyde) in root and shoot tissues were determined separately by standard protocol.
Result: Under arsenic treatment oxidative stress was induced by overproduction of reactive oxygen species (ROS) and disruption of antioxidant defense system measured in terms of increased activity of antioxidant enzymes and lipid peroxidation (malondialdehyde) in root and shoot tissues separately. Eugenol-treated seedlings along with arsenic exposure substantially decreased the level of arsenic uptake in plants resulting in a substantial reduction in ROS overproduction and MDA content. SOD, CAT, GPX activities perform an influential role in arsenic stress acclimatization and eugenol treated seedlings with arsenic exposures indicated substantial changes in all variables evaluated as compared to arsenic treatment only. This study suggests that oxidative stress caused by arsenic was ameliorated by eugenol.
- Aebi Hugo. (1984). Catalase in vitro. Methods in Enzymology. 105: 121-126.
- Anjum, S.A., Tanveer, M., Hussain, S., Shahzad, B., Ashraf, U., Fahad, S., Hassan,W., Jan, S., Khan, I., Saleem, M.F. (2016). Osmo-regulation and antioxidant production in maize under combined cadmium and arsenic stress. Environ SciPollut Res. 23(12): 11864-75.
- Beauchamp, C., Fridovich, I. (1971). Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276-287.
- Bezerra, D.P., Gardenia Carmen Gadelha Militão, Mayara Castro De Morais and Damião Pergentino De Sousa. (2017). The Dual Antioxidant/Prooxidant Effect of Eugenol and Its Action in Cancer Development and Treatment Nutrients. 9: 1367.3 0f15.
- Carty Mc, Hanh, K.M., H.T., Kim, K.W. (2011). Arsenic geochemistry and human health in South East Asia. Rev. Environ. Health. 26: 71-78.
- Chandrakar, V., Parkhey, S., Dubey, A., Keshavkant, S. (2017b). Modulation in arsenic-induced lipid catabolism in Glycine max L. using proline, 24-epibrassinolide and diphenyleneio- -donium. Biologia. 72: 292-299.
- Dave, R., Tripathi, R.D., Dwivedi, S., Tripathi,P., Dixit, G., Sharma, Y.K., Trivedi, P.K., Corpas, F.J., J.B. Barroso, J.B., Chakrabarty, D. (2013). Arsenate and arsenite exposure modulate antioxidants and amino acids in contrasting arsenic accumulating rice (Oryza sativa L.) genotypes. J. Hazard. Mater. 262: 1123-1131. [CrossRef] [PubMed].
- Gautam A., Pandey, K.A., Dubey, S.R. (2019). Azadirachta indica and Ocimum sanctum leaf extracts alleviate arsenic toxicity by reducing arsenic uptake and improving the antioxidant system in rice seedlings. Physiol Mol Biol Plants. 26(1): 63-81.
- Hammer Schmidt, R., Nucleus, E.M. and Kuc, J. (1982). Association of enhanced peroxidize activity with induced systemic resistance of cucumber to Colletotrichumlagenarium. Phystological Plant Pathology. 20: 73-82.
- Hartley-Whitaker, J., Ainsworth, G., Meharg, A.A. (2001). Copper and arsenate-induced oxidative stress in Holicus lanatus L. Clones with differential sensitivity. Plant Cell Environ, 24: 713-722.
- Hodges, D.M., Long, De., Forney, J.M., Prange, C.F., R.K. (1999). Improving the thiobarbituric acid-reactive-substances assay as for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta. 207: 604-611.
- Kaur, G., Athar, M., Alam, M.S. (2010). Eugenol precludes cutaneous chemical carcinogenesis in a mouse by preventing oxidative stress and inflammation and by inducing apoptosis. Mol. Carcinog. 49: 290-301.
- Li, W.X., Chen, T.B. Huang Z.C., Lei, M., Liao., X.Y. (2006). Effect of arsenic on chloroplast ultrastructure and calcium distribution in arsenic hyperaccumulator Pterisvittata L. Chemosphere. 62: 803-809.
- Mandal, B.K., Suzuki, K.T. (2002). Arsenic round the world: A review. Talanta. 58: 201-235.
- Mairaj S., Nagar, R.D., Chauhan, A., Rehman, F., Jindal, T. (2020). Impact of arsenic toxicity on oryza sativa L. and its amelioration using eugenole. Journal of Critical Reviews. 07(19): 10143-47.
- Mehmood, T.,Bibi, I., Shahid ,M., Niazi, N.K., Murtaza, B., Wang, H.,OK., Y.S., Sarkar, B., Javed, M.T., Murtaza, G.( 2017). Effect of compost addition on arsenic uptake, morphological and physiological attributes of maize plants grown in contrasting soils. J. Geochem. Explore. 178: 83-91.
- Mehrag, A.A., (2004). Arsenic in rice-understanding a new disaster for South-East Asia. Trends Plant Sci. 9: 415-417.
- Nakano and Asada, K. (1987).Purification of ascorbate peroxidase in spinach chloroplast as inactivation in ascorbate depleted medium and reactivation by monodehydroascorbate radical. Plant and Cell Physiology. 28: 131-140.
- Singh, A.P., Dixit, G., Mishra, S., Dwivedi,S., Tiwari, M., Mallick, S., Tripathi, R.D. (2015). Salicylic acid modulates arsenic toxicity by reducing its root-to-shoot translocation in rice (Oryza sativa L.). Front. Plant Sci. 6: 340.
- Srivastava, S., Mishra, S. Tripathi, R.D., Dwivedi, S., Trivedi, P.K., Tandon, P.K. (2007). Pytochelatins and antioxidant systems respond differentially during arsenite and arsenate stress in Hydrillaverticillata. Royle. Environ. Sci. Technol. 41: 2930-2936.
- Souri, Z., Karimi, N., de Oliveira, L.M. (2017). Antioxidant enzyme responses in shoots of arsenic hyperaccumulator, Isatis Cappadocia Desv, under the interaction of arsenate and phosphate. Environ.Technol. 2017: 1-32. [CrossRef] [PubMed]
- Srivastava, M., Ma, L.Q., N. Singh, N., Singh, S. (2005). Antioxidant responses of hyperaccumulator and sensitive fern species to arsenic. J. Exp. Bot. 56: 1332-1342.
- Waalkes, M.P., Liu, J., Ward, J.M., Diwan, B.A. (2004). Mechanisms underlying arsenic carcinogenesis: Hypersensitivity of mice exposed to inorganic arsenic during gestation. Toxicology. 198: 31-38.
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