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Application of Response Surface Methodology for Optimization of Siderophore Production

DOI: 10.18805/IJARe.A-5663    | Article Id: A-5663 | Page : 230-237
Citation :- Application of Response Surface Methodology for Optimization of Siderophore Production.Indian Journal of Agricultural Research.2022.(56):230-237
R.U. Raje Nimbalkar, N.S. Barge, R.J. Marathe, Y.B. Phatake, R.B. Deshmukh, S.S. Dange, S.G. Mane, S.S. Dhawan rjndrmarathe@gmail.com
Address : Department of Microbiology, ADT’s, Shardabai Pawar Mahila Arts, Commerce and Science College, Shardanagar, Baramati-413 115, Maharashtra, India.
Submitted Date : 31-07-2020
Accepted Date : 30-10-2020

Abstract

Background: In the present study a statistical model (Response Surface Methodology) was proposed for optimization of siderophore production by using Enterobacter hormaechei.
Methods: The rhizospheric soil was used for isolation and isolates were screened for siderophore production by chrome-azurol S (CAS) assay. One potent isolate producing maximum siderophore was selected and characterized by 16S rRNA gene sequencing. The culture conditions were optimized for maximum siderophore production by using Central Composite Design. The response surface curves were used to predict the optimum levels of the factors affecting the yield of siderophore. 
Result: By using rhizospheric soil,eight isolates were obtained and one potent organism was identified as Enterobacter hormaechei subsp. oharae (Accession No. MT 775835) by BLAST. The maximum siderophore production (98%) was obtained in succinate medium and the optimum values of variables were found as pH 7, time 60 hrs, temp. 28°C and succinic acid conc. 0.40%. RSM was used to analyze the data by developing 3D surface plots and the residuals plots. ANOVA was used to determine regression coefficients.

Keywords

ANOVA Central composite design Chrome-azurol S Enterobacter hormaechei subsp. oharae Regression

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