Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

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Indian Journal of Agricultural Research, volume 55 issue 4 (august 2021) : 488-492

Entomopathogenic Fungal Screening against Two Spotted Spider Mites, Tetranychus urticae Koch in Tomato and Broad Mite, Polyphagotarsonemus latus (Banks) in Chilli

S. Sumaiya Parveen, K. Ramaraju, S. Jeyarani
1Karunya Institute of Technology and Sciences, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India.
Cite article:- Parveen Sumaiya S., Ramaraju K., Jeyarani S. (2021). Entomopathogenic Fungal Screening against Two Spotted Spider Mites, Tetranychus urticae Koch in Tomato and Broad Mite, Polyphagotarsonemus latus (Banks) in Chilli. Indian Journal of Agricultural Research. 55(4): 488-492. doi: 10.18805/IJARe.A-5661.
Background: Entomopathogenic fungi are microorganisms that specifically infect and often kill insects and other arthropods. Most are non-pathogenic to plants and relatively non-toxic to humans and animals. Use of fungal entomopathogens can be an alternative to insecticides and could help in bio-intensive pest management. The current study aimed to study the pathogenicity of entomopathogenic fungal isolates against two Spotted Spider Mites, Tetranychus urticae Koch in tomato and broad Mite, Polyphagotarsonemus latus (Banks) in chilli. 
Methods: An extensive survey for the natural occurrence of entomopathogenic fungi on insects and mites was made in different agro climatic regions of Tamil Nadu during 2013 and 2014. Survey revealed the occurrence of two entomopathogenic fungi viz., Beauveria bassiana (Balsamo) Vuillemin and Metarhizium flavoviride Gams and Rozsypal var. minus. Laboratory experiment was conducted to evaluate the pathogenicity of fourteen isolates of Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii against Tetranychus urticae and Polyphagotarsonemus latus
Result: In opposition to T. urticae, the Beauveria bassiana isolate Bb 112 showed the highest toxicity of all the other isolates tested, with low LC50 value of 0.6 x106 spores ml-1 and LT50 of 92.60h. Against P. latus, Bb 112 had high virulence with low LC50 value of 0.3x106 spores ml-1 and LT50 value of 92.32h. Hence, there is potential for use of entomopathogenic fungi in the field conditions as an alternate control method in combating the insect pests and other arthropod pests since they are considered natural mortality agents and are environmentally safe.
  1. Alves, S.B., Rossi, L.S., Biaggoni, L.R., Tamai, M.A. and Pereira, P.M. (2002). Beauveria bassiana yeast phase on agar medium and its pathogenicity against Diatraea saccharalis (Lepidoptera: Crambidae) and Tetranychus urticae (Acari: Tetranychidae). J. Invertebr. Pathol. 81(2): 70-77.
  2. Draganova, S.A. and Simova, S.A. (2010). Susceptibility of Tetranychus urticae Koch. (Acari: Tetranychidae) to isolates of entomopathogenic fungus Beauveria bassiana. Pestic. Phytomed. 25(1): 51-57.
  3. Gatarayiha, M.C., Laing, M.D. and Miller, R.M. (2012). Selection of Beauveria bassiana strains against the two spotted spider mite, Tetranychus urticae Koch in laboratory and greenhouse trials. Afr. J. Microbiol. Res. 6(11): 2694-2703.
  4. Irigaray, F.J.S., Marco-Mancebon, V. and Perez-Moreno, I. (2003). The entomopathogenic fungus Beauveria bassiana and its compatibility with triflumuron: effect on the two-spotted spider mite, Tetranychus urticae. Biol. Control. 26(2): 168-173.
  5. Jaihan, P., Sangdee, K. and Sangdee, A. (2016). Selection of entomopathogenic fungus for biological control of chili anthracnose disease caused by Colletotrichum spp. Eur. J. Plant Pathol. 146: 551-564. 
  6. Lovett, B. and Leger, R.J.S. (2017) The insect pathogens. Microbiol Spectr. ASM Press, Washington, DC. p 925-943.
  7. Maketon, M., Coghlan, P.O. and Sinprasert, J. (2008). Evaluation of Metarhizium anisopliae (Deuteromycota: Hyphomycetes) for control of broad mite Polyphagotarsonemus latus (Acari: Tarsonemidae) in mulberry. Exp. Appl. Acarol. 46: 157-167.
  8. Mustafa, U. and Kaur, G. (2008). Entomopathogenic fungi as potent biocontrol agents. J. environ. Res. and Dev. 3(2): 569-574.
  9. Muthukumar. (2005). Possibilities of using fungal pathogens against two spotted spider mite, Tetranychus urticae Koch on okra. M.Sc. (Ag.) Thesis, Tamil Nadu Agricultural University, Coimbatore. India
  10. Nugroho, I. and Ibrahim, Y. (2004). Laboratory bioassay of some entomopathogenic fungi against Broad Mite (Polyphagotarsonemus latus Bank). Int. J. Agri. Biol. 6(2): 223-225.
  11. Pena, J.E. and Osborne, L. (1996). Biological control of Polyphagotarsonemus latus (Acarina: Tarsonemidae) in greenhouses and field trials using introductions of predacious mites (Acarina: Phytoseiidae). Entomophaga. 41: 279-285.
  12. Rios-Moreno, A., Garrido-Jurado, I. and Resquín-Romero, G. (2016). Destruxin A production by Metarhizium brunneum strains during transient endophytic colonisation of Solanum tuberosum. Biocontrol Sci Technol. 26: 1574-1585. 
  13. Roberts, D.W. (1981). Toxins of entomopathogenic fungi. In: Microbial Control of Pests and Plant Diseases. ed. H.D. Burges, Academic Press, London, 441-464p.
  14. Sangeetha, S. (2013). Microbial control of two spotted spider mite, Tetranychus urticae Koch on tomato and broad mite, Polyphagotarsonemus latus (Banks) on chilli, Ph. D. Thesis, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
  15. Seiedy, M., Saboori, A., Allahyari, H., Hassanloui, R.T. and Tork, M. (2010). Laboratory investigation on the virulence of two isolates of the entomopathogenic fungus Beauveria bassiana against the twospotted spider mite Tetranychus urticae (Acari: Tetranychidae). Int. J. Acarol. 36(6): 527-532.

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